Difference between revisions of "Team:TCFSH Taiwan/Results"
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| − | + | <div class="topic"><p class="text_color">UV</p></div> | |
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<p class="content"><br>(1) 32ﾟC, 200rpm, culture12hr (15ml) *continue to log phase</br><br>(2) add (15ml) LB *re-culture</br><br>(3) TeﾟC, continue cultural</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p> | <p class="content"><br>(1) 32ﾟC, 200rpm, culture12hr (15ml) *continue to log phase</br><br>(2) add (15ml) LB *re-culture</br><br>(3) TeﾟC, continue cultural</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p> | ||
| − | + | <p class="title">UV</p> | |
| − | + | <p class="content-1">Culture setting</p> | |
| − | + | <p class="content"><br>(1) 37ﾟC, 200rpm, culture 12hr (15ml) *continue to log phase</br><br>(2) Add (15ml) LB *re-culture</br><br>(3) re-culture at 37ﾟC under UV</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p> | |
| − | + | <p class="content-1">Expression measurement setting</p> | |
| + | <p class="content"><br>(1) Extract: Take (3ml) bacteria liquid, and centrifuge at 14000rpm, 1min</br><br>(2) add 1ml PBS, pipetting+vortex</br><br>(3) measure the o.d. value</br></p> | ||
</div> | </div> | ||
</section> | </section> | ||
Revision as of 06:47, 1 November 2017
