|
|
| Line 1: |
Line 1: |
| − | {{SDSZ-China}}
| |
| − | <html>
| |
| | | | |
| − | <head>
| |
| − | <title>Project</title>
| |
| − |
| |
| − | <style type='text/css'>
| |
| − | #top_title, #sideMenu {
| |
| − | display: none;
| |
| − | }
| |
| − |
| |
| − | #content {
| |
| − | width: 100%;
| |
| − | margin: 0;
| |
| − | padding: 0;
| |
| − | background: #f3f4f4;
| |
| − | }
| |
| − |
| |
| − | p {
| |
| − | font-family: Lato !important;
| |
| − | font-size: 17px !important
| |
| − | }
| |
| − |
| |
| − | h1 {
| |
| − | font-size: 40 -px !important;
| |
| − | }
| |
| − |
| |
| − | body {
| |
| − | font-family: Lato !important;
| |
| − | }
| |
| − |
| |
| − | h3 {
| |
| − | font-size: 25 -px;
| |
| − | }
| |
| − |
| |
| − | nav {
| |
| − | margin-left: -40px;
| |
| − | margin-top: -40px;
| |
| − | }
| |
| − |
| |
| − | #bg {
| |
| − | position: fixed;
| |
| − | z-index: -1;
| |
| − | top: 0;
| |
| − | left: 0;
| |
| − | }
| |
| − | </style>
| |
| − | <link href='http://fonts.googleapis.com/css?family=Lato' rel='stylesheet' type='text/css'>
| |
| − | <!-- 最新版本的 Bootstrap 核心 CSS 文件 -->
| |
| − | <link rel="stylesheet" href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css"
| |
| − | integrity="sha384-BVYiiSIFeK1dGmJRAkycuHAHRg32OmUcww7on3RYdg4Va+PmSTsz/K68vbdEjh4u" crossorigin="anonymous">
| |
| − |
| |
| − |
| |
| − | <script>localStorage.clear();</script>
| |
| − | <!-- 最新的 Bootstrap 核心 JavaScript 文件 -->
| |
| − | <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"
| |
| − | integrity="sha384-Tc5IQib027qvyjSMfHjOMaLkfuWVxZxUPnCJA7l2mCWNIpG9mGCD8wGNIcPD7Txa"
| |
| − | crossorigin="anonymous"></script>
| |
| − |
| |
| − | <script type="text/javascript" async
| |
| − | src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-MML-AM_CHTML"></script>
| |
| − | <script type="text/x-mathjax-config">
| |
| − | MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]}});
| |
| − |
| |
| − |
| |
| − |
| |
| − | </script>
| |
| − |
| |
| − | <script type="text/javascript">
| |
| − |
| |
| − | $(document).ready(function () {
| |
| − | $("#category_navbar a").on('click', function (event) {
| |
| − | // Make sure this.hash has a value before overriding default behavior
| |
| − | if (this.hash !== "") {
| |
| − | // Prevent default anchor click behavior
| |
| − | event.preventDefault();
| |
| − |
| |
| − | // Store hash0
| |
| − | var hash = this.hash;
| |
| − |
| |
| − | // Using jQuery's animate() method to add smooth page scroll
| |
| − | // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area
| |
| − | $('html, body').animate({
| |
| − | scrollTop: $(hash).offset().top
| |
| − | }, 800, function () {
| |
| − |
| |
| − | // Add hash (#) to URL when done scrolling (default click behavior)
| |
| − | window.location.hash = hash;
| |
| − | });
| |
| − | } // End if
| |
| − | });
| |
| − | });
| |
| − |
| |
| − |
| |
| − | </script>
| |
| − |
| |
| − | </head>
| |
| − | </head>
| |
| − |
| |
| − | <body data-spy="scroll" data-target="#category_navbar">
| |
| − |
| |
| − | <div class="container" style='z-index:10;position:relative;'>
| |
| − |
| |
| − |
| |
| − | <div class="row card" id='bodycontainer' class='row'>
| |
| − |
| |
| − | <nav class="navbar navbar-default navbar-static-top">
| |
| − | <!-- Brand and toggle get grouped for better mobile display -->
| |
| − | <div class="navbar-header">
| |
| − | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
| |
| − | data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
| |
| − | <span class="sr-only">Toggle navigation</span>
| |
| − | <span class="icon-bar"></span>
| |
| − | <span class="icon-bar"></span>
| |
| − | <span class="icon-bar"></span>
| |
| − | </button>
| |
| − | <a class="navbar-brand" href="#">Brand</a>
| |
| − | </div>
| |
| − |
| |
| − | <!-- Collect the nav links, forms, and other content for toggling -->
| |
| − | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
| |
| − | <ul class="nav navbar-nav">
| |
| − | <li><a href="/Team:SDSZ-China">Home</a></li>
| |
| − | <li><a href="/Team:SDSZ-China/Project">Project</a></li>
| |
| − | <li><a href="/Team:SDSZ-China/Parts">Parts</a></li>
| |
| − | <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
| |
| − | <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
| |
| − | </ul>
| |
| − | <ul class="nav navbar-nav navbar-right">
| |
| − | <li><a href="/Team:SDSZ-China/Team">Team</a></li>
| |
| − | <li><a href="#">By SDSZ</a></li>
| |
| − | </ul>
| |
| − | </div><!-- /.navbar-collapse -->
| |
| − | </nav>
| |
| − |
| |
| − |
| |
| − | <div class="col-sm-2" style='padding-left:5% padding-right:10%'>
| |
| − | <div id="category_navbar">
| |
| − | <ul class="nav nav-list" data-spy="affix" data-offset-top="160"
| |
| − | style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
| |
| − |
| |
| − | <li><a href="#Background">Background</a></li>
| |
| − | <li><a href="#Purpose">Purpose</a></li>
| |
| − | <li><a href="#Overview">Overview of the project</a></li>
| |
| − | <li><a href="#Goal">Our goal</a></li>
| |
| − |
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − |
| |
| − | <div class="col-sm-10" style="padding-right:5%">
| |
| − | <div class="row">
| |
| − | <div class="col-sm-12">
| |
| − | <h2 id='Background'>Background</h2>
| |
| − | <p>Bear bile, one of the most famous animal drugs in Traditional Chinese Medicine (TCM), has been
| |
| − | recorded in ancient Chinese medicine book as a significant method to treat hepatic and biliary
| |
| − | disorders. UDCA, the effective ingredient of bear bile.Aside from the traditional use of bear
| |
| − | bile in Chinese medicine, UDCA(ursodeoxycholic acid), the effective ingredient of bear bile
| |
| − | acid, has a much larger pharmaceutical application. As well as the usage of UDCA in dissolving
| |
| − | gallstone, its efficacy in primary biliary cirrhosis and primary sclerosing cholangitis (PSC) as
| |
| − | an adjunct to medical therapy has been well established. Newer indications include its use in
| |
| − | the management of chronic hepatitis, cirrhosis, post liver transplant rejection,
| |
| − | graft-versus-host disease and acute viral hepatitis, where it not only relieves symptoms of
| |
| − | cholestasis but also arrests ongoing hepatocyte necrosis. However, the increasing demand for
| |
| − | bear bile has caused bears to be in an endangered state: bear poaching and illegal animal trade
| |
| − | have greatly dwindled the number of the wild Asiatic black bear. Apart from that, bear bile
| |
| − | farming industry in Asia extracts bile through “milking” from the bears, which is operated
| |
| − | through surgically implanting a permanent catheter in the animal's gallbladder to obtain the
| |
| − | drips. It is unquestionable that the bear bile farming process will lead to both physical and
| |
| − | psychological damage in bears. </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row">
| |
| − | <div class="col-sm-12">
| |
| − | <h2 id='Purpose'>Purpose</h2>
| |
| − | <p>To find substitute or alternative for bear bile farming, our team will be working on the
| |
| − | biological synthesis of the main effective component of this important medicine,
| |
| − | UDCA(Ursodeoxycholic Acid). This biological approach will not only be more efficient but also be
| |
| − | cheaper than the original chemical approach, which is widely used in the current UDCA synthesis
| |
| − | industry. </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row">
| |
| − | <div class="col-sm-12">
| |
| − | <h2 id='Overview'>Overview of the project</h2>
| |
| − | <p>We found that it is possible to convert the main component of poultry bile, CDCA(Chenodeoxycholic
| |
| − | Acid), into UDCA, by employing two enzyme-catalyzed the reactions. First, two enzymes was
| |
| − | employed to manage the transformation of CDCA to 7oxo-LCA. In the present of
| |
| − | 7a-HSD(7alpha-hydroxysteroid dehydrogenase), CDCA is transformed in to 7oxo-LCA by loosing a
| |
| − | pair of hydrogen(2H+ and 2e-), the pair of hydrogen is added to NAD+, the cofactor and the
| |
| − | acceptor. The NAD+ is transformed into NADH during the reaction. To regenerate the NAD+ and
| |
| − | recycle the reaction, to , the LDH(Lactate dehydrogenase) works on pyruvate and take the pair of
| |
| − | hydrogen from NADH and transform the pyruvate to lactate and NADH to NAD+.</p>
| |
| − | <p>In the second step, the 7oxo-LCA is transformed to UDCA by 7β-HSDH(7beta-hydroxysteroid
| |
| − | dehydrogenase)and GDH(glutamate dehydrogenase).The 7β-HSDH works on 7oxo-LCA and take a pair of
| |
| − | hydrogen from NADPH(the cofactor for the second step)and add it to 7oxo-LCA and form a beta
| |
| − | position 7-hydroxyl group, which is our target product UDCA. The GDH works on glucose and take a
| |
| − | pair of hydrogen from it and add it to the NADP+, to form NADPH to manage the regeneration of
| |
| − | cofactor NADPH for the second step.</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="row">
| |
| − | <div class="col-sm-12">
| |
| − | <h2 id='Goal'>Our goal</h2>
| |
| − | <p>Our mission is to expression of the four enzymes 7α-HSDH (from ecoli DH5a), 7β-HSDH (from
| |
| − | Ruminococcus Torques), GDH (from Bacillus subtilis), and LDH from (Lactobacillus delbruechii
| |
| − | subsp. Bulgaricus)and test their activities. By adding the CBD( cellulose binding
| |
| − | domain)sequnence to the plasmid we construct, we manage to bind our enzyme on gauze. This
| |
| − | specific design excels in 3 specific ways: first, by controlling the presence of the gauze in
| |
| − | the solution, we can control the process of the reaction. Second, when the target enzyme is
| |
| − | bound to cellulose we manage to purify the protein we express. Third, the enzyme binding gauze
| |
| − | is employed to a machine including the reaction efficiency measuring system and the enzyme
| |
| − | addition controlling system .</p>
| |
| − | </div>
| |
| − | </div>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="backgroundpic">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/e/e5/T--TAS_Taipei--Taipei_Background_1.0.jpg" width="100%"
| |
| − | height="100%" id="bg" alt="">
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </body>
| |
| − |
| |
| − | </html>
| |
