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<p>Visit <a href="https://2017.igem.org/Team:DTU-Denmark/ExperimentalDesign">experimental design</a> for the theory and design behind our experiments, and <a href="https://2017.igem.org/Team:DTU-Denmark/Protocols">protocols</a> for the protocols of our experiments. | <p>Visit <a href="https://2017.igem.org/Team:DTU-Denmark/ExperimentalDesign">experimental design</a> for the theory and design behind our experiments, and <a href="https://2017.igem.org/Team:DTU-Denmark/Protocols">protocols</a> for the protocols of our experiments. | ||
</p><br /> | </p><br /> | ||
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+ | <h2 id="amc">AMC Experiment</h2> | ||
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+ | <p>In this experiment, we used the AMC fluorescent molecule, coupled with a peptide sequence (A-L-K) known to be cleaved by serine proteases from literature. The molecule emits fluorescence when it is released from the peptide it is coupled with. For that reason, we expected to see fluorescence when the peptide was cleaved by proteases in the snake venom. We first generated a standard curve of the AMC molecule without the peptide sequence as seen in figure 1. | ||
+ | </p><br /> | ||
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+ | <figure> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/ec/T--DTU-Denmark--AMC_standardcurve.png" alt="AMC standard curve" width="500"> | ||
+ | <figcaption>Figure 1: AMC standardcurve</figcaption> | ||
+ | </figure> | ||
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</div> | </div> | ||
Revision as of 14:13, 1 November 2017
Results
Visit experimental design for the theory and design behind our experiments, and protocols for the protocols of our experiments.
AMC Experiment
In this experiment, we used the AMC fluorescent molecule, coupled with a peptide sequence (A-L-K) known to be cleaved by serine proteases from literature. The molecule emits fluorescence when it is released from the peptide it is coupled with. For that reason, we expected to see fluorescence when the peptide was cleaved by proteases in the snake venom. We first generated a standard curve of the AMC molecule without the peptide sequence as seen in figure 1.
![AMC standard curve](https://static.igem.org/mediawiki/2017/e/ec/T--DTU-Denmark--AMC_standardcurve.png)