Difference between revisions of "Team:SJTU-BioX-Shanghai/Loader"
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<div class="col-12 col-lg-9"> | <div class="col-12 col-lg-9"> | ||
<div class="my-title h5-my-responsive" id="section1">Intruduction</div> | <div class="my-title h5-my-responsive" id="section1">Intruduction</div> | ||
| − | <p> | + | <p>We first seek for a suitable loader for our engineered bacteria, after we had the plan to make a product. In order to make bacteria fixed and to present a clear color for photography and color analysis, we considered three possible loaders. We had three potentials evaluated. Finally, we decided to take glass fiber filter paper as our loader.</p> |
| − | + | ||
<div class="my-title h5-my-responsive" id="section2">Chip</div> | <div class="my-title h5-my-responsive" id="section2">Chip</div> | ||
| − | <p> | + | <p>We got the idea of combining chip with our project when communicating with Prof. Xiang Chen from Micro/Nano Research Institute of SJTU. Chip provided by their lab is a flexible carrier with a micro chamber which is made of PDMS material. The micro chamber has a height of approximately 80-100 μm and can be filled with 2-3 μL of solution to the maximum.</p> |
| − | <p> | + | <p>Chip is a small-sized and low-cost carrier. It can be combined with a box holding a smart phone. The box can further be applied to photo analysis with the chip（Kanakasabapathy, M. K., et.al，2017）. We planned to have a combination with smart phone since smart phone enjoys greater popularity and most products have a tendency to be moblized. Meanwhile, chip is light and small, whose characteristics exactly fit our need for making a portable tool.</p> |
| + | <p>To demonstrate the feasibility of the chip, we carried out experiment. We added 2μL mutant RFP solution into the chip chamber and observed the color. As is shown in Fig.1, color is indistinguishable wherever observed, under naked eye or in picture. We then tried to have centrifuged hoping the color will be more distinctive. However, the color is still hard to tell (Fig.2).</p> | ||
| + | <p>图片1</p> | ||
| + | <p>Fig.1 （a）Chip photographed by mobile phone. Solution is added into the labelled chamber on the right. (b) The color of 1mL solution inside an ep tube</p> | ||
| + | <p>图片2</p> | ||
| + | <p>Fig.2 (a) Chip photographed by mobile phone. Solution is added into the labelled chamber on the left. (b) The color of the centrifugal solution inside an ep tube</p> | ||
| + | <p>We supposed that the reason why color was not distinctive was the small size of the chamber. So we put our chip under microscope to take a look. It is shown in Fig.3 that it is difficult to distinguish red color from the picture. Though red color can be observed on the wall of the chamber after centrifugation (Annotation: Centrifugation is a process which involves the application of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. ) of 4k rpm, chip is not suitable for photo analysis.</p> | ||
| + | <p>Besides weak color presentation, another negative characteric of the chip is tough condition for long-term culture of bacteria. The reaction period of our engineered bacteria will last several hours, so the culture medium inside the chip may not be able to support the normal production of bacteria.</p> | ||
| + | <p>图片3</p> | ||
| + | <p>Fig.3 （a）RFP solution observed under microscope without centrifugation（b）RFP solution observed under microscope after centrifugation of 2k rpm（c）RFP solution observed under microscope after centrifugation of 4k rpm</p> | ||
| + | <p>Picture（a）,（b）,（c）is exposed for 4ms, at 10x magnification.</p> | ||
<div class="my-title h5-my-responsive" id="section3">Microdroplet</div> | <div class="my-title h5-my-responsive" id="section3">Microdroplet</div> | ||
<p>Something something something something something something something something something</p> | <p>Something something something something something something something something something</p> | ||
Revision as of 14:56, 1 November 2017
LoaderWe first seek for a suitable loader for our engineered bacteria, after we had the plan to make a product. In order to make bacteria fixed and to present a clear color for photography and color analysis, we considered three possible loaders. We had three potentials evaluated. Finally, we decided to take glass fiber filter paper as our loader.
We got the idea of combining chip with our project when communicating with Prof. Xiang Chen from Micro/Nano Research Institute of SJTU. Chip provided by their lab is a flexible carrier with a micro chamber which is made of PDMS material. The micro chamber has a height of approximately 80-100 μm and can be filled with 2-3 μL of solution to the maximum.
Chip is a small-sized and low-cost carrier. It can be combined with a box holding a smart phone. The box can further be applied to photo analysis with the chip（Kanakasabapathy, M. K., et.al，2017）. We planned to have a combination with smart phone since smart phone enjoys greater popularity and most products have a tendency to be moblized. Meanwhile, chip is light and small, whose characteristics exactly fit our need for making a portable tool.
To demonstrate the feasibility of the chip, we carried out experiment. We added 2μL mutant RFP solution into the chip chamber and observed the color. As is shown in Fig.1, color is indistinguishable wherever observed, under naked eye or in picture. We then tried to have centrifuged hoping the color will be more distinctive. However, the color is still hard to tell (Fig.2).
图片1
Fig.1 （a）Chip photographed by mobile phone. Solution is added into the labelled chamber on the right. (b) The color of 1mL solution inside an ep tube
图片2
Fig.2 (a) Chip photographed by mobile phone. Solution is added into the labelled chamber on the left. (b) The color of the centrifugal solution inside an ep tube
We supposed that the reason why color was not distinctive was the small size of the chamber. So we put our chip under microscope to take a look. It is shown in Fig.3 that it is difficult to distinguish red color from the picture. Though red color can be observed on the wall of the chamber after centrifugation (Annotation: Centrifugation is a process which involves the application of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. ) of 4k rpm, chip is not suitable for photo analysis.
Besides weak color presentation, another negative characteric of the chip is tough condition for long-term culture of bacteria. The reaction period of our engineered bacteria will last several hours, so the culture medium inside the chip may not be able to support the normal production of bacteria.
图片3
Fig.3 （a）RFP solution observed under microscope without centrifugation（b）RFP solution observed under microscope after centrifugation of 2k rpm（c）RFP solution observed under microscope after centrifugation of 4k rpm
Picture（a）,（b）,（c）is exposed for 4ms, at 10x magnification.
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