Difference between revisions of "Team:Aix-Marseille/Amelioration part:BBa K1445000"
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Results weren’t good, after a transformation using TG1 bacteria with the [http://parts.igem.org/Part:BBa_K1445000 BBa_K1445000], we picked up the colonies on a chloramphenicol plate and infected them with our M13KO7 phage like particle. | Results weren’t good, after a transformation using TG1 bacteria with the [http://parts.igem.org/Part:BBa_K1445000 BBa_K1445000], we picked up the colonies on a chloramphenicol plate and infected them with our M13KO7 phage like particle. | ||
| − | After that, we executed the [ | + | After that, we executed the [Team:Aix-Marseille/Experiments/Protocols#Production_of_large_quantities_of_helper_phage|Large quantities of helper phage] protocol and infected GM1 bacteria with the resulting phage like particle. |
After an overnight grow, we counted the colonies. | After an overnight grow, we counted the colonies. | ||
Revision as of 16:32, 1 November 2017
M13 Amelioration part
As we use a phage like particle, we needed to use the M13 phagemid. Results weren’t good, after a transformation using TG1 bacteria with the [http://parts.igem.org/Part:BBa_K1445000 BBa_K1445000], we picked up the colonies on a chloramphenicol plate and infected them with our M13KO7 phage like particle.
After that, we executed the [Team:Aix-Marseille/Experiments/Protocols#Production_of_large_quantities_of_helper_phage|Large quantities of helper phage] protocol and infected GM1 bacteria with the resulting phage like particle.
After an overnight grow, we counted the colonies. Here are the results for the M13 from the BBa_K1445000, and the pBlueScript II plasmid that we used:
In this experiment, for better results, everything was done three times, and these results are averages.
