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− | Methanotrophs are special bacteria which utilizes one-carbon-compounds (methane, methanol, formaldehyde) as carbon source. Methanococcus capsulatus Bath is a Gram-negative, coccoid aerob methanotroph bacteria which tends to form diplococcus shape by its capsule. The metabolism, genome and transformation possibilities of this species has been studied by many research teams in the last three decades. Several transformed strains are patented in several countries with the aim to produce organic compounds from methane. | + | Methanotrophs are special bacteria which utilizes one-carbon-compounds (methane, methanol, formaldehyde) as carbon source. Methanococcus capsulatus Bath is a Gram-negative, coccoid aerob methanotroph bacteria which tends to form diplococcus shape by its capsule. The metabolism, genome and transformation possibilities of this species has been studied by many research teams in the last three decades. Several transformed strains are patented in several countries with the aim to produce organic compounds from methane.</p5> |
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+ | <h1><left>The Product</left></h1> | ||
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We would like to create a methane biosensor using methane utilizing bacteria called Methylococcus capsulatus. Our final product would be bacterial medium which changes it’s colour in the presence of methane. | We would like to create a methane biosensor using methane utilizing bacteria called Methylococcus capsulatus. Our final product would be bacterial medium which changes it’s colour in the presence of methane. | ||
During a sequel of chemical reactions our bacteria converts methane into pyruvic acid, however the widely spread enzyme, lactate dehydrogenase (LDH) is not produced by Methylococcus. As we would like to measure the lactate concentration, we have to add the gene of LDH to Methylococcus capsulatus’ DNA. For that process we will conduct ourselves towards bacterial transformation using plasmids. If we succeed, our bacteria will produce lactate out of methane. | During a sequel of chemical reactions our bacteria converts methane into pyruvic acid, however the widely spread enzyme, lactate dehydrogenase (LDH) is not produced by Methylococcus. As we would like to measure the lactate concentration, we have to add the gene of LDH to Methylococcus capsulatus’ DNA. For that process we will conduct ourselves towards bacterial transformation using plasmids. If we succeed, our bacteria will produce lactate out of methane. | ||
The lactate produced this way can turn the medium’s colour with the help of an assay kit, thus making methane detection possible. Lactate, created by our method, can also be used for the production of polylactate also by the bacteria. Consequently further changes in the bacteria would allow it to directly produce polylactate from methane. </p5> | The lactate produced this way can turn the medium’s colour with the help of an assay kit, thus making methane detection possible. Lactate, created by our method, can also be used for the production of polylactate also by the bacteria. Consequently further changes in the bacteria would allow it to directly produce polylactate from methane. </p5> | ||
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Revision as of 16:56, 1 November 2017