Nagahama

Protocol

Our Lab's Protocols

Medium

LB medium (100 mL liquid)

1.Measure 1g Tripton

2.Measure 0.5g Yeast Extract

3.Measure 1g Nacl

4.Add 100mL H2O

5.autoclave(121℃ 20min)

YPD medium(100 mL liquid)

1.Measure 2g Polypepton

2.Measure 1g Yeast Extract

3.Measure 2g Glucose

4.Add 100mL H2O

5.autoclave(121℃ 20min)

DNA work

Agarose gel(100mL)

Method of Making 0.7% Agarose gel

1.Measure 0.7g Agarose

2.Add 100mL TAE buffer

3.Heat(till agarose melted)*We used a microwave oven.

4.Pur agarose into a gel maker

5.Set a comb

6.Wait till agarose curdles

7.Pull an comb