|
|
| Line 10: |
Line 10: |
| | <meta name=Generator content="Microsoft Word 14"> | | <meta name=Generator content="Microsoft Word 14"> |
| | <meta name=Originator content="Microsoft Word 14"> | | <meta name=Originator content="Microsoft Word 14"> |
| − | <link rel=File-List href="PROToCOLS_files/filelist.xml"> | + | <link rel=File-List href="INTER_files/filelist.xml"> |
| − | <link rel=Edit-Time-Data href="PROToCOLS_files/editdata.mso"> | + | <link rel=Edit-Time-Data href="INTER_files/editdata.mso"> |
| | <!--[if !mso]> | | <!--[if !mso]> |
| | <style> | | <style> |
| Line 24: |
Line 24: |
| | <o:LastAuthor>hp</o:LastAuthor> | | <o:LastAuthor>hp</o:LastAuthor> |
| | <o:Revision>1</o:Revision> | | <o:Revision>1</o:Revision> |
| − | <o:TotalTime>8</o:TotalTime> | + | <o:TotalTime>2</o:TotalTime> |
| − | <o:Created>2017-11-01T20:38:00Z</o:Created> | + | <o:Created>2017-11-01T21:25:00Z</o:Created> |
| − | <o:LastSaved>2017-11-01T20:50:00Z</o:LastSaved> | + | <o:LastSaved>2017-11-01T21:27:00Z</o:LastSaved> |
| − | <o:Pages>2</o:Pages> | + | <o:Pages>1</o:Pages> |
| − | <o:Words>340</o:Words> | + | <o:Words>2</o:Words> |
| − | <o:Characters>1941</o:Characters> | + | <o:Characters>16</o:Characters> |
| − | <o:Lines>16</o:Lines> | + | <o:Lines>1</o:Lines> |
| − | <o:Paragraphs>4</o:Paragraphs> | + | <o:Paragraphs>1</o:Paragraphs> |
| − | <o:CharactersWithSpaces>2277</o:CharactersWithSpaces> | + | <o:CharactersWithSpaces>17</o:CharactersWithSpaces> |
| | <o:Version>14.00</o:Version> | | <o:Version>14.00</o:Version> |
| | </o:DocumentProperties> | | </o:DocumentProperties> |
| Line 39: |
Line 39: |
| | </o:OfficeDocumentSettings> | | </o:OfficeDocumentSettings> |
| | </xml><![endif]--> | | </xml><![endif]--> |
| − | <link rel=dataStoreItem href="PROToCOLS_files/item0001.xml" | + | <link rel=dataStoreItem href="INTER_files/item0001.xml" |
| − | target="PROToCOLS_files/props002.xml"> | + | target="INTER_files/props002.xml"> |
| − | <link rel=themeData href="PROToCOLS_files/themedata.thmx"> | + | <link rel=themeData href="INTER_files/themedata.thmx"> |
| − | <link rel=colorSchemeMapping href="PROToCOLS_files/colorschememapping.xml"> | + | <link rel=colorSchemeMapping href="INTER_files/colorschememapping.xml"> |
| | <!--[if gte mso 9]><xml> | | <!--[if gte mso 9]><xml> |
| | <w:WordDocument> | | <w:WordDocument> |
| Line 345: |
Line 345: |
| | /* Font Definitions */ | | /* Font Definitions */ |
| | @font-face | | @font-face |
| − | {font-family:Wingdings;
| |
| − | panose-1:5 0 0 0 0 0 0 0 0 0;
| |
| − | mso-font-charset:2;
| |
| − | mso-generic-font-family:auto;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:0 268435456 0 0 -2147483648 0;}
| |
| − | @font-face
| |
| − | {font-family:Wingdings;
| |
| − | panose-1:5 0 0 0 0 0 0 0 0 0;
| |
| − | mso-font-charset:2;
| |
| − | mso-generic-font-family:auto;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:0 268435456 0 0 -2147483648 0;}
| |
| − | @font-face
| |
| | {font-family:Calibri; | | {font-family:Calibri; |
| | panose-1:2 15 5 2 2 2 4 3 2 4; | | panose-1:2 15 5 2 2 2 4 3 2 4; |
| Line 365: |
Line 351: |
| | mso-font-pitch:variable; | | mso-font-pitch:variable; |
| | mso-font-signature:-520092929 1073786111 9 0 415 0;} | | mso-font-signature:-520092929 1073786111 9 0 415 0;} |
| − | @font-face
| |
| − | {font-family:Tahoma;
| |
| − | panose-1:2 11 6 4 3 5 4 4 2 4;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:swiss;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:-520081665 -1073717157 41 0 66047 0;}
| |
| − | @font-face
| |
| − | {font-family:"Book Antiqua";
| |
| − | panose-1:2 4 6 2 5 3 5 3 3 4;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:roman;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:647 0 0 0 159 0;}
| |
| − | @font-face
| |
| − | {font-family:Algerian;
| |
| − | panose-1:4 2 7 5 4 10 2 6 7 2;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:decorative;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:3 0 0 0 1 0;}
| |
| − | @font-face
| |
| − | {font-family:"Open Sans";
| |
| − | panose-1:0 0 0 0 0 0 0 0 0 0;
| |
| − | mso-font-alt:"Times New Roman";
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:roman;
| |
| − | mso-font-format:other;
| |
| − | mso-font-pitch:auto;
| |
| − | mso-font-signature:0 0 0 0 0 0;}
| |
| | /* Style Definitions */ | | /* Style Definitions */ |
| | p.MsoNormal, li.MsoNormal, div.MsoNormal | | p.MsoNormal, li.MsoNormal, div.MsoNormal |
| Line 416: |
Line 372: |
| | mso-bidi-font-family:"Times New Roman"; | | mso-bidi-font-family:"Times New Roman"; |
| | mso-bidi-theme-font:minor-bidi;} | | mso-bidi-theme-font:minor-bidi;} |
| − | a:link, span.MsoHyperlink
| |
| − | {mso-style-priority:99;
| |
| − | color:blue;
| |
| − | text-decoration:underline;
| |
| − | text-underline:single;}
| |
| − | a:visited, span.MsoHyperlinkFollowed
| |
| − | {mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | color:purple;
| |
| − | mso-themecolor:followedhyperlink;
| |
| − | text-decoration:underline;
| |
| − | text-underline:single;}
| |
| − | p
| |
| − | {mso-style-priority:99;
| |
| − | mso-margin-top-alt:auto;
| |
| − | margin-right:0in;
| |
| − | mso-margin-bottom-alt:auto;
| |
| − | margin-left:0in;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:12.0pt;
| |
| − | font-family:"Times New Roman","serif";
| |
| − | mso-fareast-font-family:"Times New Roman";
| |
| − | mso-fareast-theme-font:minor-fareast;}
| |
| − | p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
| |
| − | {mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | mso-style-link:"Balloon Text Char";
| |
| − | margin:0in;
| |
| − | margin-bottom:.0001pt;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:8.0pt;
| |
| − | font-family:"Tahoma","sans-serif";
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;}
| |
| − | p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | margin-top:0in;
| |
| − | margin-right:0in;
| |
| − | margin-bottom:10.0pt;
| |
| − | margin-left:.5in;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;}
| |
| − | p.MsoListParagraphCxSpFirst, li.MsoListParagraphCxSpFirst, div.MsoListParagraphCxSpFirst
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-type:export-only;
| |
| − | margin-top:0in;
| |
| − | margin-right:0in;
| |
| − | margin-bottom:0in;
| |
| − | margin-left:.5in;
| |
| − | margin-bottom:.0001pt;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;}
| |
| − | p.MsoListParagraphCxSpMiddle, li.MsoListParagraphCxSpMiddle, div.MsoListParagraphCxSpMiddle
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-type:export-only;
| |
| − | margin-top:0in;
| |
| − | margin-right:0in;
| |
| − | margin-bottom:0in;
| |
| − | margin-left:.5in;
| |
| − | margin-bottom:.0001pt;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;}
| |
| − | p.MsoListParagraphCxSpLast, li.MsoListParagraphCxSpLast, div.MsoListParagraphCxSpLast
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-type:export-only;
| |
| − | margin-top:0in;
| |
| − | margin-right:0in;
| |
| − | margin-bottom:10.0pt;
| |
| − | margin-left:.5in;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;}
| |
| − | span.BalloonTextChar
| |
| − | {mso-style-name:"Balloon Text Char";
| |
| − | mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-locked:yes;
| |
| − | mso-style-link:"Balloon Text";
| |
| − | mso-ansi-font-size:8.0pt;
| |
| − | mso-bidi-font-size:8.0pt;
| |
| − | font-family:"Tahoma","sans-serif";
| |
| − | mso-ascii-font-family:Tahoma;
| |
| − | mso-hansi-font-family:Tahoma;
| |
| − | mso-bidi-font-family:Tahoma;}
| |
| − | span.SpellE
| |
| − | {mso-style-name:"";
| |
| − | mso-spl-e:yes;}
| |
| − | span.GramE
| |
| − | {mso-style-name:"";
| |
| − | mso-gram-e:yes;}
| |
| | .MsoChpDefault | | .MsoChpDefault |
| | {mso-style-type:export-only; | | {mso-style-type:export-only; |
| Line 582: |
Line 396: |
| | div.WordSection1 | | div.WordSection1 |
| | {page:WordSection1;} | | {page:WordSection1;} |
| − | /* List Definitions */
| |
| − | @list l0
| |
| − | {mso-list-id:17397210;
| |
| − | mso-list-type:hybrid;
| |
| − | mso-list-template-ids:-154127740 67698689 67698691 67698693 67698689 67698691 67698693 67698689 67698691 67698693;}
| |
| − | @list l0:level1
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0B7;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:Symbol;}
| |
| − | @list l0:level2
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:o;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:"Courier New";}
| |
| − | @list l0:level3
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0A7;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:Wingdings;}
| |
| − | @list l0:level4
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0B7;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:Symbol;}
| |
| − | @list l0:level5
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:o;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:"Courier New";}
| |
| − | @list l0:level6
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0A7;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:Wingdings;}
| |
| − | @list l0:level7
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0B7;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:Symbol;}
| |
| − | @list l0:level8
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:o;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:"Courier New";}
| |
| − | @list l0:level9
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0A7;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:Wingdings;}
| |
| − | @list l1
| |
| − | {mso-list-id:527305042;
| |
| − | mso-list-type:hybrid;
| |
| − | mso-list-template-ids:1506328854 -268911842 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
| |
| − | @list l1:level1
| |
| − | {mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;
| |
| − | font-family:"Times New Roman","serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | color:black;
| |
| − | mso-themecolor:text1;
| |
| − | mso-ansi-font-weight:bold;}
| |
| − | @list l1:level2
| |
| − | {mso-level-number-format:alpha-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l1:level3
| |
| − | {mso-level-number-format:roman-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:right;
| |
| − | text-indent:-9.0pt;}
| |
| − | @list l1:level4
| |
| − | {mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l1:level5
| |
| − | {mso-level-number-format:alpha-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l1:level6
| |
| − | {mso-level-number-format:roman-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:right;
| |
| − | text-indent:-9.0pt;}
| |
| − | @list l1:level7
| |
| − | {mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l1:level8
| |
| − | {mso-level-number-format:alpha-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l1:level9
| |
| − | {mso-level-number-format:roman-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:right;
| |
| − | text-indent:-9.0pt;}
| |
| − | @list l2
| |
| − | {mso-list-id:869534640;
| |
| − | mso-list-type:hybrid;
| |
| − | mso-list-template-ids:366508600 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
| |
| − | @list l2:level1
| |
| − | {mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l2:level2
| |
| − | {mso-level-number-format:alpha-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l2:level3
| |
| − | {mso-level-number-format:roman-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:right;
| |
| − | text-indent:-9.0pt;}
| |
| − | @list l2:level4
| |
| − | {mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l2:level5
| |
| − | {mso-level-number-format:alpha-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-.25in;}
| |
| − | @list l2:level6
| |
| − | {mso-level-number-format:roman-lower;
| |
| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:right;
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| − | text-indent:-9.0pt;}
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| − | @list l2:level7
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| − | {mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;}
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| − | @list l2:level8
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| − | {mso-level-number-format:alpha-lower;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;}
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| − | @list l2:level9
| |
| − | {mso-level-number-format:roman-lower;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:right;
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| − | text-indent:-9.0pt;}
| |
| − | @list l3
| |
| − | {mso-list-id:938374804;
| |
| − | mso-list-type:hybrid;
| |
| − | mso-list-template-ids:-786953526 67698689 67698691 67698693 67698689 67698691 67698693 67698689 67698691 67698693;}
| |
| − | @list l3:level1
| |
| − | {mso-level-number-format:bullet;
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| − | mso-level-text:\F0B7;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:Symbol;}
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| − | @list l3:level2
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| − | {mso-level-number-format:bullet;
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| − | mso-level-text:o;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:"Courier New";}
| |
| − | @list l3:level3
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| − | {mso-level-number-format:bullet;
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| − | mso-level-text:\F0A7;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:Wingdings;}
| |
| − | @list l3:level4
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| − | {mso-level-number-format:bullet;
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| − | mso-level-text:\F0B7;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
| |
| − | font-family:Symbol;}
| |
| − | @list l3:level5
| |
| − | {mso-level-number-format:bullet;
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| − | mso-level-text:o;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:"Courier New";}
| |
| − | @list l3:level6
| |
| − | {mso-level-number-format:bullet;
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| − | mso-level-text:\F0A7;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:Wingdings;}
| |
| − | @list l3:level7
| |
| − | {mso-level-number-format:bullet;
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| − | mso-level-text:\F0B7;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:Symbol;}
| |
| − | @list l3:level8
| |
| − | {mso-level-number-format:bullet;
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| − | mso-level-text:o;
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| − | mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
| |
| − | font-family:"Courier New";}
| |
| − | @list l3:level9
| |
| − | {mso-level-number-format:bullet;
| |
| − | mso-level-text:\F0A7;
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| − | mso-level-tab-stop:none;
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| − | mso-level-number-position:left;
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| − | text-indent:-.25in;
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| − | font-family:Wingdings;}
| |
| − | ol
| |
| − | {margin-bottom:0in;}
| |
| − | ul
| |
| − | {margin-bottom:0in;}
| |
| | --> | | --> |
| | </style> | | </style> |
| Line 851: |
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| | </style> | | </style> |
| | <![endif]--><!--[if gte mso 9]><xml> | | <![endif]--><!--[if gte mso 9]><xml> |
| − | <o:shapedefaults v:ext="edit" spidmax="1026"/> | + | <o:shapedefaults v:ext="edit" spidmax="1027"/> |
| | </xml><![endif]--><!--[if gte mso 9]><xml> | | </xml><![endif]--><!--[if gte mso 9]><xml> |
| | <o:shapelayout v:ext="edit"> | | <o:shapelayout v:ext="edit"> |
| Line 858: |
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| | </head> | | </head> |
| | | | |
| − | <body lang=EN-US link=blue vlink=purple style='tab-interval:.5in'> | + | <body lang=EN-US style='tab-interval:.5in'> |
| | | | |
| | <div class=WordSection1> | | <div class=WordSection1> |
| | | | |
| | <p class=MsoNormal align=center style='text-align:center'><span | | <p class=MsoNormal align=center style='text-align:center'><span |
| − | style='font-size:72.0pt;line-height:115%;font-family:Algerian'>PROToCOLS<o:p></o:p></span></p> | + | style='font-size:56.0pt;line-height:115%'>INTER-LAB STUDY<o:p></o:p></span></p> |
| − | | + | |
| − | <p align=center style='margin-top:0in;margin-right:0in;margin-bottom:0in;
| + | |
| − | margin-left:.5in;margin-bottom:.0001pt;text-align:center;text-indent:-.25in;
| + | |
| − | mso-list:l1 level1 lfo1'><![if !supportLists]><b style='mso-bidi-font-weight:
| + | |
| − | normal'><span style='font-size:14.0pt;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;color:black;
| + | |
| − | mso-themecolor:text1;mso-font-kerning:12.0pt'><span style='mso-list:Ignore'>1.<span
| + | |
| − | style='font:7.0pt "Times New Roman"'> </span></span></span></b><![endif]><b><span
| + | |
| − | style='font-size:20.0pt;font-family:"Calibri","sans-serif";mso-ascii-theme-font:
| + | |
| − | minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;
| + | |
| − | color:black;mso-themecolor:text1;mso-font-kerning:12.0pt'>Protocol for Cloning
| + | |
| − | Experiments</span></b><span style='font-size:14.0pt;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
| + | |
| − | mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:text1;mso-font-kerning:
| + | |
| − | 12.0pt'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;
| + | |
| − | margin-bottom:.0001pt;text-align:justify;text-indent:-.25in;line-height:150%;
| + | |
| − | mso-list:l0 level1 lfo4'><![if !supportLists]><span style='font-size:14.0pt;
| + | |
| − | line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
| + | |
| − | Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span><![endif]><span style='font-size:14.0pt;line-height:150%;
| + | |
| − | font-family:"Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-bidi-font-family:
| + | |
| − | "Times New Roman";mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:
| + | |
| − | text1;mso-font-kerning:12.0pt'>Protocol for PCR Amplification, Competent Cell
| + | |
| − | Preparation, and Transformation was followed as given in Molecular Cloning: A
| + | |
| − | Laboratory Manual, 3<span class=SpellE><sup><span style='position:relative;
| + | |
| − | top:-8.5pt;mso-text-raise:8.5pt'>rd</span></sup></span><span
| + | |
| − | style='mso-spacerun:yes'> </span>edition Sam brook.</span><span
| + | |
| − | style='font-size:14.0pt;line-height:150%'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;
| + | |
| − | margin-bottom:.0001pt;text-align:justify;text-indent:-.25in;line-height:150%;
| + | |
| − | mso-list:l0 level1 lfo4'><![if !supportLists]><span style='font-size:14.0pt;
| + | |
| − | line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
| + | |
| − | Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span><![endif]><span style='font-size:14.0pt;line-height:150%;
| + | |
| − | font-family:"Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-bidi-font-family:
| + | |
| − | "Times New Roman";mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:
| + | |
| − | text1;mso-font-kerning:12.0pt'>All Restriction Endonuclease used are of Thermo
| + | |
| − | Fisher Scientific and T4DNA ligation Enzyme is of New England Bio labs and all
| + | |
| − | reactions are set as per guidelines of the supplier.</span><span
| + | |
| − | style='font-size:14.0pt;line-height:150%'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin:0in;margin-bottom:.0001pt'><span style='font-size:14.0pt'><o:p> </o:p></span></p>
| + | |
| − | | + | |
| − | <p align=center style='margin-top:0in;margin-right:0in;margin-bottom:0in;
| + | |
| − | margin-left:.5in;margin-bottom:.0001pt;text-align:center;text-indent:-.25in;
| + | |
| − | mso-list:l1 level1 lfo1'><![if !supportLists]><b style='mso-bidi-font-weight:
| + | |
| − | normal'><span style='font-size:20.0pt;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;color:black;
| + | |
| − | mso-themecolor:text1'><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span></b><![endif]><b><span style='font-size:20.0pt;font-family:
| + | |
| − | "Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-bidi-font-family:
| + | |
| − | "Times New Roman";mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:
| + | |
| − | text1;mso-font-kerning:12.0pt'>Promoter Characterization</span></b><span
| + | |
| − | style='font-size:20.0pt'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin:0in;margin-bottom:.0001pt;text-align:justify;line-height:150%'><span
| + | |
| − | style='font-size:14.0pt;line-height:150%;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
| + | |
| − | mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:text1;mso-font-kerning:
| + | |
| − | 12.0pt'>We had done in <i>In-Silico</i> Characterization of Promoter by
| + | |
| − | validating the <i style='mso-bidi-font-style:normal'>cis-</i>element of the
| + | |
| − | promoters by using Plant Care.</span><span style='font-size:14.0pt;line-height:
| + | |
| − | 150%'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin:0in;margin-bottom:.0001pt;text-align:justify;line-height:150%'><span
| + | |
| − | style='font-size:14.0pt;line-height:150%;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
| + | |
| − | mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:text1;mso-font-kerning:
| + | |
| − | 12.0pt'>(http://bioinformatics.psb.ugent.be/webtools/plantcare/html/)</span><span
| + | |
| − | style='font-size:14.0pt;line-height:150%'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin:0in;margin-bottom:.0001pt;text-align:justify;line-height:150%'><span
| + | |
| − | style='font-size:14.0pt;line-height:150%;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
| + | |
| − | mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:text1;mso-font-kerning:
| + | |
| − | 12.0pt'>Functional validation of characterized MMV promoter via protoplast assay
| + | |
| − | in tobacco had done by expertise of the plant biotechnology lab, Department of
| + | |
| − | Botany D.E.I, <span class=SpellE>Dayalbagh</span>, <span class=GramE>Agra,</span>
| + | |
| − | Protocol had not been shared due to publication and patenting issues.<o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p align=center style='margin-top:0in;margin-right:0in;margin-bottom:0in;
| + | |
| − | margin-left:.5in;margin-bottom:.0001pt;text-align:center;text-indent:-.25in;
| + | |
| − | mso-list:l1 level1 lfo1'><![if !supportLists]><b><span style='font-size:20.0pt;
| + | |
| − | font-family:"Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-fareast-font-family:
| + | |
| − | Calibri;color:black;mso-themecolor:text1;mso-font-kerning:12.0pt'><span
| + | |
| − | style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'> </span></span></span></b><![endif]><b><span
| + | |
| − | style='font-size:20.0pt;font-family:"Calibri","sans-serif";mso-ascii-theme-font:
| + | |
| − | minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;
| + | |
| − | color:black;mso-themecolor:text1;mso-font-kerning:12.0pt'>Protein
| + | |
| − | Characterization<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;
| + | |
| − | margin-bottom:.0001pt;text-align:justify;text-indent:-.25in;mso-list:l3 level1 lfo2'><![if !supportLists]><span
| + | |
| − | style='font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:Symbol;
| + | |
| − | mso-bidi-font-family:Symbol;color:#0D0D0D;mso-themecolor:text1;mso-themetint:
| + | |
| − | 242'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span><![endif]><span style='font-size:14.0pt;color:#0D0D0D;
| + | |
| − | mso-themecolor:text1;mso-themetint:242;mso-font-kerning:12.0pt'>Quantity
| + | |
| − | Estimation of Protein: For quantify the expressed protein Bradford Method is
| + | |
| − | used as given on the following link </span><span class=GramE><span
| + | |
| − | style='font-size:14.0pt;color:#0D0D0D;mso-themecolor:text1;mso-themetint:242'>-(</span></span><a
| + | |
| − | href="http://www.bio-protocol.org/e45"><span style='font-size:14.0pt;
| + | |
| − | color:#0D0D0D;mso-themecolor:text1;mso-themetint:242'>http://www.bio-protocol.org/e45</span></a><span
| + | |
| − | style='font-size:14.0pt;color:#0D0D0D;mso-themecolor:text1;mso-themetint:242;
| + | |
| − | mso-font-kerning:12.0pt'>).</span><span style='font-size:14.0pt;color:#0D0D0D;
| + | |
| − | mso-themecolor:text1;mso-themetint:242'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;
| + | |
| − | margin-bottom:.0001pt;text-align:justify;text-indent:-.25in;mso-list:l3 level1 lfo2'><![if !supportLists]><span
| + | |
| − | style='font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:Symbol;
| + | |
| − | mso-bidi-font-family:Symbol;color:#0D0D0D;mso-themecolor:text1;mso-themetint:
| + | |
| − | 242'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span><![endif]><span style='font-size:14.0pt;color:#0D0D0D;
| + | |
| − | mso-themecolor:text1;mso-themetint:242;mso-font-kerning:12.0pt'>Quality
| + | |
| − | Estimation of protein:<span style='mso-spacerun:yes'> </span>The quality of
| + | |
| − | expressed protein was done by SDS-PAGE.</span><span style='font-size:14.0pt;
| + | |
| − | color:#0D0D0D;mso-themecolor:text1;mso-themetint:242'><o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;
| + | |
| − | margin-bottom:.0001pt;text-align:justify;text-indent:-.25in;mso-list:l3 level1 lfo2'><![if !supportLists]><span
| + | |
| − | class=MsoHyperlink><span style='font-size:14.0pt;font-family:Symbol;mso-fareast-font-family:
| + | |
| − | Symbol;mso-bidi-font-family:Symbol;color:#0D0D0D;mso-themecolor:text1;
| + | |
| − | mso-themetint:242;text-decoration:none;text-underline:none'><span
| + | |
| − | style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span></span><![endif]><span style='font-size:14.0pt;color:#0D0D0D;
| + | |
| − | mso-themecolor:text1;mso-themetint:242;mso-font-kerning:12.0pt'>Protocol for
| + | |
| − | the SD</span><span style='font-size:14.0pt;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
| + | |
| − | mso-bidi-theme-font:minor-bidi;color:#0D0D0D;mso-themecolor:text1;mso-themetint:
| + | |
| − | 242;mso-font-kerning:12.0pt'>S-PAGE is followed as given on the following
| + | |
| − | web-link- (</span><a
| + | |
| − | href="http://www.assay-protocol.com/molecular-biology/electrophoresis/denaturing-page"><span
| + | |
| − | style='font-size:14.0pt;font-family:"Book Antiqua","serif";color:#0D0D0D;
| + | |
| − | mso-themecolor:text1;mso-themetint:242'>http://www.assay-protocol.com/molecular-biology/electrophoresis/denaturing-page</span></a><span
| + | |
| − | class=MsoHyperlink><span style='font-size:14.0pt;font-family:"Book Antiqua","serif";
| + | |
| − | color:#0D0D0D;mso-themecolor:text1;mso-themetint:242'>)</span></span><span
| + | |
| − | class=MsoHyperlink><span style='font-size:14.0pt;color:#0D0D0D;mso-themecolor:
| + | |
| − | text1;mso-themetint:242;text-decoration:none;text-underline:none'><o:p></o:p></span></span></p>
| + | |
| − | | + | |
| − | <p style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;
| + | |
| − | margin-bottom:.0001pt;text-align:justify'><span class=MsoHyperlink><span
| + | |
| − | style='font-size:14.0pt;color:#0D0D0D;mso-themecolor:text1;mso-themetint:242;
| + | |
| − | text-decoration:none;text-underline:none'><o:p> </o:p></span></span></p>
| + | |
| − | | + | |
| − | <p align=center style='margin-top:0in;margin-right:0in;margin-bottom:0in;
| + | |
| − | margin-left:.5in;margin-bottom:.0001pt;text-align:center;text-indent:-.25in;
| + | |
| − | mso-list:l1 level1 lfo1'><![if !supportLists]><b style='mso-bidi-font-weight:
| + | |
| − | normal'><span style='font-size:20.0pt;font-family:"Calibri","sans-serif";
| + | |
| − | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;color:black;
| + | |
| − | mso-themecolor:text1'><span style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'>
| + | |
| − | </span></span></span></b><![endif]><b><span style='font-size:20.0pt;font-family:
| + | |
| − | "Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-bidi-font-family:
| + | |
| − | "Times New Roman";mso-bidi-theme-font:minor-bidi;color:black;mso-themecolor:
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| − | text1;mso-font-kerning:12.0pt'>Immobilization of Cells Expressing our Protein
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| − | of Interest</span></b><span style='font-size:20.0pt'><o:p></o:p></span></p>
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| − | color:black;mso-themecolor:text1;mso-font-kerning:12.0pt'>Cells of<i> E. Coli</i>
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| − | Bl-21 expressing our desired protein are immobilized using the protocol given
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| − | by <span class=SpellE>Dhall</span> et al., 2012.</span><span style='font-size:
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| − | color:black;mso-themecolor:text1;mso-font-kerning:12.0pt'>(http://sci-hub.cc/http://pubs.rsc.org/en/content/articlelanding/2012/ay/c2ay25960e/unauth#!divAbstract</span><span
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| − | mso-list:l1 level1 lfo1'><![if !supportLists]><b style='mso-bidi-font-weight:
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| − | mso-themecolor:text1'><span style='mso-list:Ignore'>5.<span style='font:7.0pt "Times New Roman"'>
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| − | </span></span></span></b><![endif]><b><span style='font-size:20.0pt;font-family:
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| − | text1;mso-font-kerning:12.0pt'>Efficacy Testing of Beads by treating with water
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| − | samples possessing heavy metals contamination</span></b><span style='font-size:
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| − | <p style='margin:0in;margin-bottom:.0001pt'><span style='font-size:14.0pt;
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| − | text1;mso-font-kerning:12.0pt'>Concentration of heavy metals in samples treated
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| − | with our fabricated bio-beads (BECO) are analyzed by ICP-<span class=GramE>OES<span
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| − | style='mso-spacerun:yes'> </span>of</span> Agilent Technology</span><span
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| − | color:white;mso-themecolor:light1;mso-font-kerning:12.0pt'>.</span><span
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| − | style='font-size:20.0pt'><o:p> </o:p></span></p>
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| − | <p class=MsoNormal>References:<o:p></o:p></p>
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| − | style='mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin'><span
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| − | style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'>
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| − | </span></span></span><![endif]><span class=SpellE>Dhall</span> P, Kumar R and
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| − | Kumar A. 2012. BOD beads – a ready to use seeding material for estimation of
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| − | organic load of wastewater. Anal. Methods, 2012, 4, 4101. DOI:
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| − | 10.1039/c2ay25960e<o:p></o:p></p>
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| − | </span></span></span><![endif]><span style='font-size:10.5pt;line-height:115%;
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| − | font-family:"Open Sans","serif";color:black;background:white'>He, F. (2011).
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| − | Bradford Protein Assay. <em><span style='font-family:"Open Sans","serif";
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| − | mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi'>Bio-protocol</span></em> Bio101:
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| − | e45. DOI: </span><a href="https://doi.org/10.21769/BioProtoc.45"><span
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| − | style='font-size:10.5pt;line-height:115%;font-family:"Open Sans","serif";
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| − | color:#0094FF;background:white'>10.21769/BioProtoc.45</span></a><span
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| − | style='font-size:10.5pt;line-height:115%;font-family:"Open Sans","serif";
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