Difference between revisions of "Team:Purdue/Composite Part"

Line 1: Line 1:
 
{{Purdue}}
 
{{Purdue}}
 
<html>
 
<html>
 +
</div>
 +
 +
<p> <h4>BnzA<h4> <p>
 +
<p>Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol.</p>
 +
</div>
 +
<p> <a href="http://parts.igem.org/Part:BBa_K2228001">Link to registry</a> </p>
 +
 +
 +
 +
<p> <h4>BnzB<h4> <p>
 +
<p>Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol. This subunit is responsible for substrate specificity.</p>
 +
</div>
 +
<p> <a href="http://parts.igem.org/Part:BBa_K2228002">Link to registry</a> </p>
 +
 +
 +
 +
<p> <h4>BnzC<h4> <p>
 +
<p>Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol. This is the ferredoxin subunit. </p>
 +
</div>
 +
<p> <a href="http://parts.igem.org/Part:BBa_K2228003">Link to registry</a> </p>
 +
 +
<p> <h4>BnzD<h4> <p>
 +
<p>Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol. This is the ferredoxin-NAD reductase subunit. </p>
 +
</div>
 +
<p> <a href="http://parts.igem.org/Part:BBa_K2228004">Link to registry</a> </p>
  
 +
<p> <h4>CatE<h4> <p>
 +
<p>Codes for cis-1,2-dihydrobenzene-1,2-diol dehydrogenase which catalyzes the conversion of catechol to 2-hydroxymuconate-6-semialdehyde. </p>
 
</div>
 
</div>
−
<p> <h4>Upper Operon<h4> <p>
+
<p> <a href="http://parts.igem.org/Part:BBa_K2228005">Link to registry</a> </p>
  
−
<p>The part contains a TetR coding region under the control of a constitutive promoter and an operon under the control of a TetR promoter. The operon encodes for a three enzyme- six gene pathway that converts benzene to 2-hydroxymuconate-6-semialdehyde, going through cis-1,2-dihydrobenzene-1,2-diol and catechol as intermediates. This pathway can then be combined with a separate pathway (device unsubmitted) to convert 2-hydroxymuconate-6-semialdehyde to the final products of pyruvate and acetyl-CoA. The enzymes used are benzene-1,2 dioxygenase, cis-1,2-dihydrobenzene-1,2-diol dehydrogenase, and catechol-2,3-dioxygenase. We refer to this pathway as the "upper operon," and the unsubmitted pathway as the "lower operon," which together degrade benzene for incorporation into metabolism. The whole pathway was split into two operons due to their large size.</p>
+
<p> <h4>BnzE<h4> <p>
 +
<p>Codes for cis-1,2-dihydrobenzene-1,2-diol dehydrogenase which converts cis-1-2-dihydrobenzene-1,2-diol to catechol. </p>
 
</div>
 
</div>
 +
<p> <a href="http://parts.igem.org/Part:BBa_K2228006">Link to registry</a> </p>
  
−
<p> <a href="http://parts.igem.org/Part:BBa_K2228000">Link to registry</a> </p>
 
  
  

Revision as of 21:30, 1 November 2017

BnzA

Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol.

Link to registry

BnzB

Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol. This subunit is responsible for substrate specificity.

Link to registry

BnzC

Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol. This is the ferredoxin subunit.

Link to registry

BnzD

Codes for a subunit of a complex that converts benzene to cis-1,2-dihydrobenzene-1,2-diol. This is the ferredoxin-NAD reductase subunit.