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| − | <span dir="auto">Team:BIT/Design</span>
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| | <a style="color:#1b8fac">Project</a> | | <a style="color:#1b8fac">Project</a> |
| | <ul class="dropdown"> | | <ul class="dropdown"> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Design">Design</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/design">Design</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Demonstrate">Demonstrate</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/demonstrate">Demonstrate</a></li> |
| | <li><a href="https://2017.igem.org/Team:BIT/Model">Model</a></li> | | <li><a href="https://2017.igem.org/Team:BIT/Model">Model</a></li> |
| | </ul> | | </ul> |
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| | <a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a> | | <a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a> |
| | <ul class="dropdown"> | | <ul class="dropdown"> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Medal">Medal</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/medal">Medal</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Prize">Prize</a></li> | + | <li><a href="2017.igem.org/Team:BIT/prize">Prize</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Parts">Part</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/part">Part</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/https://2017.igem.org/Team:BIT/Safety">Safety Form</a></li>
| + | |
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| | </li> | | </li> |
| | | | |
| | <li class="has-dropdown"> | | <li class="has-dropdown"> |
| − | <a href="https://2017.igem.org/Team:BIT/HP">Practice</a> | + | <a href="https://2017.igem.org/Team:BIT/achievement">Practice</a> |
| | <ul class="dropdown"> | | <ul class="dropdown"> |
| − | <li><a href="https://2017.igem.org/Team:BIT/HP/Silver">Silver</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/design">Silver</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/HP/Gold_Integrated">Gold</a></li> | + | <li><a href="2017.igem.org/Team:BIT/demonstrate">Gold</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Engagement"> Engagement</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/Model">Education & Public Engagement</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Entrepreneurship">Entreperneurship</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/Model">Entreperneurship</a></li> |
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| | <li class="has-dropdown"> | | <li class="has-dropdown"> |
| − | <a href="https://2017.igem.org/Team:BIT/Notebook">Notebook</a> | + | <a href="https://2017.igem.org/Team:BIT/achievement">Notebook</a> |
| | <ul class="dropdown"> | | <ul class="dropdown"> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Gallery">Gallery</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/design">Gallery</a></li> |
| − | <li><a href="2017.igem.org/Team:BIT/Track">Track</a></li> | + | <li><a href="2017.igem.org/Team:BIT/demonstrate">Track</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/nb">Journal</a></li> | + | <li><a href="https://2017.igem.org/Team:BIT/Model">Journal</a></li> |
| − | <li><a href="https://2017.igem.org/Team:BIT/Protocol">Protocol</a></li>
| + | |
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| | <div class="row animate-box"> | | <div class="row animate-box"> |
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| − | <h2>Safety</h2> | + | <h2>Design</h2> |
| − | <p><font size=6>Safety of the laborers<br> | + | <p><font size=4>This year's project, using microfluidic chip as a platform, equipped with genetic engineering ideas and molecular cloning means, ultimately achieve the purpose that using biosensors for biomarker detection.<p>Our chip consists of two chambers:<dr> |
| − | <font size=3>In order to ensure the safety of the experimental staffs, not just our team members, but also professors,teachers and senior schoolmates. To prevent us from blowing up the laboratory, the laboratory responsible for the safety of the teacher deliberately spent a whole day for us a laboratory safety training: including the introduction of laboratory safety rules and regulations, the use of various instruments and laboratory standards Health requirements, and when we have just started the experiment, please refer to the laboratory of the use of our equipment.<br> | + | <p>Chamber 1 is oval(long axis length:18mm, short axis length:6mm). In our design, the function of the chamber is to hold our magnetic beads (fixed with magnets) with the aptamer and provide a place for the sample to bind to the aptamer so that the the complementary strands with lysine can get detached . Our intention to design the chamber as an oval is that the elongated structure can reduce the bubble generated by the difference in flow rate between the cavity wall and the middle stream.<dr> |
| − | <font size=6>Safety of experimental materials<br>
| + | <p>Chamber 2 is round( diameter:12mm) Within this chamber ,there are gel pillars(diameter: 0.5mm). The contents of the chamber are engineering bacteria frozen into dry powdery and trypsin. After the reaction in the chamber is carried out for a certain period of time, the mixed reaction of the culture medium and the reaction liquid flows from the upstream into the chamber under the negative pressure generated by the peristaltic pump<dr> |
| − | <font size=3>1. Engineering bacteria: the use of the project for the lysine-deficient Escherichia coli, in the absence of lysine environment can not grow, it will not affect the environment;<br> | + | <p>First of all, we select aptamers AP273, which has best affinity for AFP. We modified a biotin at the end of its 5’ end, which has affinity for streptavidin. Because of the presence of streptavidin-modified beads, aptamer has been locked firmly on beads. At the same time, a complementary chain was synthesized at its protein binding site. We modified an amino group at the 3’ end of the complementary chain which can be combined with lysine protected by BOC anhydride to link lysine to the complementary chain. Because Van der Waals forces between AFP and aptamers is stronger than hydrogen bond, due to the action of magnet lying at the bottom of the reaction system, aptamers are separated from complementary chain, one at the bottom, the other in the supernatant. At this point, with the action of trypsin, lysine can be separated from the complementary chain, and start his adventure.<dr> |
| − | 2.Part: all bio-brick used in the line from the iGEM official plasmid plate provided in the plasmid; | + | |
| − | <br>3. Chemical reagents: All chemical reagents used in the project are laboratory reagents, there is no toxicity than the laboratory prescribed reagents, there is no flammable and explosive reagents; | + | <p>In the second cavity, there is a lysine deficient E.coli which can hardly grow without the addition of lysine. Meanwhile, we transformed a plasmid with fluorescent gene into the E.coli ΔLysA. When the E.coli ΔLysA has received the lysine signal released from the complementary chain, it begins to grow with the expression of the fluorescent protein. In this way, we can transform the lysine signal to the fluorescence signal. Consider that the lysine concentration could be weak, we decided to use a strong promoter(BBa_J23100) and a cyclic amplifier system to enhance the signal, and a dual fluorescence system to improve the sensitivity.</font> |
| − | <br>4. Equipment: all the damage to the instruments are clearly classified by a dedicated person in charge of a unified recovery, sharp items can be put into the recycling container after packaging; | + | |
| − | <br>5. Waste: whether it is solid or liquid reagents, have a special waste liquid tank recycling; | + | </div> |
| − | <br>6. Personnel: Each trial has at least two students in the laboratory to ensure the safety of the experimenter; | + | </div> |
| − | <br>7. Laboratory: the last students to leave the laboratory responsible for the lock door, off the window, off the water, air conditioning and so on.<br> | + | <div class="row"> |
| − | <font size=6>Safety of project<br> | + | <div class="col-md-4"> |
| − | <font size=3>The project in the chip to respond, in a hardware device for testing. To a certain extent belong to a closed system, not easy to contact with the outside world, so compared to open the project more secure. | + | <div class="fh5co-blog animate-box"> |
| | + | <a href="#" class="blog-bg" style="background-image: url(https://static.igem.org/mediawiki/2017/f/f5/BIT_Figure_About_main_page.jpg);"></a> |
| | + | <div class="blog-text"> |
| | + | |
| | + | <h3><a href="#">Biosensor</a></h3> |
| | + | <p>1.Standardized linkers<br> |
| | + | <br> |
| | + | 2.basing on the application of aptamers<br> |
| | + | <br> |
| | + | 3.Achieving signal conversion from macromolecule to small molecule<br></p> |
| | + | <ul class="stuff"> |
| | + | |
| | + | <li><a href="https://2017.igem.org/Team:BIT/design/Biosenor">Read More<i class="icon-arrow-right22"></i></a></li> |
| | + | </ul> |
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| | + | <div class="blog-text"> |
| | + | |
| | + | <h3><a href="#">Amplifier</a></h3> |
| | + | <p>1.Transform the lysine signal to the fluorescent signal<br> |
| | + | 2.Use a strong promoter and a cyclic amplifier to enhance the signal<br> |
| | + | 3.Use a dual fluorescence system to improve the sensitivity</p> |
| | + | <ul class="stuff"> |
| | + | |
| | + | <li><a href="https://2017.igem.org/Team:BIT/design/Bio-amplifer">Read More<i class="icon-arrow-right22"></i></a></li> |
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| | + | |
| | + | <h3><a href="#">Microfluidic chip</a></h3> |
| | + | <p>1.Tiny volume and Low-cost<br> |
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