Difference between revisions of "Team:IIT Delhi/Circuit Design"

CIRCUIT DESIGN AND CONSTRUCTION

Two circuits were central to our work done in the project. The first one was the 5n1, which was a 5 node oscillator constructed by Richard Murray et al, that was not modified to produce square waves. The 5n1 (560) was a gift from Richard Murray (Addgene plasmid # 70730), and had the following topology and circuit –

Further, apart from this system, that was ordered for testing our characterization and measurement devices, we designed our own novel 5 node ring oscillator, which contained modifications that are mentioned at the end of the section. The topology and circuit that we designed and constructed are as follows –

This circuit was constructed in part through cloning, and in part through synthesis via IDT and Genscript. Further, the salient features of this circuit are –

1. Cooperativity of repressors – Cooperativity was central to our work done, in ensuring that the rise and fall is fast for our circuit. The cooperativity of the repressors in the system that we have constructed is –

NODE	REPRESSOR USED	COOPERATIVITY REPORTED
A	Orf2	6.1
B	TetR	3
C	SrpR	3.2
D	PhlF	4.5
E	BM3R1	4.5