Difference between revisions of "Team:BOKU-Vienna/Notebookdata"
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<li>Interlab challenge - transformation: According to Protocol (Preparation of chemically competent E. Coli with RbCl and transformation)</li> | <li>Interlab challenge - transformation: According to Protocol (Preparation of chemically competent E. Coli with RbCl and transformation)</li> | ||
<ul>Lisa, Mathias, Michael, Mina, Rebecca</ul> | <ul>Lisa, Mathias, Michael, Mina, Rebecca</ul> | ||
| − | <ul>8 transformations - from Kit Plate 6 InterLab Part Locations</ul> | + | <ul><li>8 transformations - from Kit Plate 6 InterLab Part Locations</li></ul> |
| − | <ul>8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)</ul> | + | <ul><li>8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)</li></ul> |
| − | <ul>stored at 35°C</ul> | + | <ul><li>stored at 35°C</li></ul> |
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 12:46, 7 July 2017
Monday, July 3rd.
- Team discussion and overview:
- Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian.
- Planning of the procedures including primer design
- Preparing the growth media and autoclaving
- LB medium preparation (4L): Mina, Michael
- Autoclaving: Mina, Rebecca
Tuesday, July 4th.
- Planning and fundraising:
- Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
- Preparation of growth media:
- 20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias
- 2 flasks LB agar: Mina, Lisa, Mathias
Wednesday, July 5th.
- Planning and fundraising:
- aliquoting 1mL LB into Eppendorf tubes
- Lisa, Josef
- Transferring Backbones onto agar plates:
- Interlab challenge - transformation: According to Protocol (Preparation of chemically competent E. Coli with RbCl and transformation)
- 8 transformations - from Kit Plate 6 InterLab Part Locations
- 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
- stored at 35°C
- Lisa, Mathias, Michael, Mina, Rebecca
