Difference between revisions of "Team:IIT Delhi/recipe"
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<a href="/Team:IIT_Delhi/Circuit_Design">Circuit design and construction</a> | <a href="/Team:IIT_Delhi/Circuit_Design">Circuit design and construction</a> | ||
| − | <a href="/Team:IIT_Delhi/Microfluidics">Microfluidics and | + | <a href="/Team:IIT_Delhi/Microfluidics">Microfluidics and Fluorescence</a> |
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Revision as of 22:21, 1 November 2017
RECIPES
A pinch of salt makes all the difference !!
20x TAE buffer
- 80mL of 1M Tris solution
- 0.7264g of EDTANa2+ salt
- 2.284 mL glacial acetic acid
- distilled H2O 100mL
- For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
- Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
- Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.
- 50 mM glucose.
- 25 mM Tris-Cl (pH 8.0).
- 10 mM EDTA (pH 8.0).
- Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by
autoclaving and store at 4°C.
- 0.2 N NaOH (freshly diluted from a 10 N stock).
- (100ul SDS + 700ul mq + 200ul NaOH) 1% (w/v) SDS.
- 5 M potassium acetate, 60.0 ml
- Glacial acetic acid, 11.5 ml
- H2O, 28.5 ml
- The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate.
Store the solution at 4°C and transfer it to an ice bucket just before use.
