We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018 Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours.

With the following protocol :

• Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL)
• To get an adequate number of colonies :
• Into 2mL LB add 2µL of 2 DO TG1
• Pipet up and down
• From the first dilution, take 8µL and dilute it into 1mL LB
• Spin at 5000rpm for 10min
• Remove 850µL supernatant
• Resuspend pellet (even if you can't see it)
• Spread remaining 150µL on petri dish
• Immediatly put on 590nm light / put control in dark
• After two hours, incubate overnight
• Count colonies

Made another control with RFP to show there's any decrease