Difference between revisions of "Team:DTU-Denmark/Parts"
| Line 58: | Line 58: | ||
<tr> | <tr> | ||
<td>BBa_K2355002</td> | <td>BBa_K2355002</td> | ||
| − | <td> | + | <td>Coding</td> |
| − | <td></td> | + | <td>This is a modification of the AmilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein</td> |
| − | <td></td> | + | <td>Philip Hau Soerensen</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355006</td> | <td>BBa_K2355006</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS</td> |
| − | <td></td> | + | <td>Chrysillis Magaard Polhaus</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355008</td> | <td>BBa_K2355008</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. </td> |
| − | <td></td> | + | <td>Cathrine Agnete Larsen </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355014</td> | <td>BBa_K2355014</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is PEDDGYFV.</td> |
| − | <td></td> | + | <td>Chrysillis Magaard Polhaus</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355015</td> | <td>BBa_K2355015</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL.</td> |
| − | <td></td> | + | <td>Cathrine Agnete Larsen </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355025</td> | <td>BBa_K2355025</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is DEMDEKSE.</td> |
| − | <td></td> | + | <td>Chrysillis Magaard Polhaus</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355026</td> | <td>BBa_K2355026</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK</td> |
| − | <td></td> | + | <td>Cathrine Agnete Larsen </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355032</td> | <td>BBa_K2355032</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is RKRRAVLT.</td> |
| − | <td></td> | + | <td>Chrysillis Magaard Polhaus</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355033</td> | <td>BBa_K2355033</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is DDSDAATF.</td> |
| − | <td></td> | + | <td>Cathrine Agnete Larsen </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355037</td> | <td>BBa_K2355037</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV.</td> |
| − | <td></td> | + | <td>Chrysillis Magaard Polhaus</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355039</td> | <td>BBa_K2355039</td> | ||
<td>Protein-domain</td> | <td>Protein-domain</td> | ||
| − | <td></td> | + | <td>This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL.</td> |
| − | <td></td> | + | <td>Cathrine Agnete Larsen </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355302</td> | <td>BBa_K2355302</td> | ||
<td>Composite part</td> | <td>Composite part</td> | ||
| − | <td></td> | + | <td>This part contains two major protein domains: A single chain avidin domain to bind the device to biotinylated surfaces, and amilCP with his-tag. Between the two domains, NgoMIV and AgeI restriction sites are present, to allow for insertion of RFC[25] part. </td> |
| − | <td></td> | + | <td>Cathrine Agnete Larsen </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K2355313</td> | <td>BBa_K2355313</td> | ||
<td>Composite part</td> | <td>Composite part</td> | ||
| − | <td></td> | + | <td>This part is used for detection of snake venom. The Beta-galactosidase, encoded by lacZ, should allow for an decrease in detection time.</td> |
| − | <td></td> | + | <td>Philip Hau Soerensen</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 00:42, 2 November 2017
Parts
| Part Number | Type | Description | Designer |
|---|---|---|---|
| BBa_K2355001 | Protein-domain | Peptide linker that is cleaved by Bitis arietans and Bitis gabonica venom. When translated, this linker should contain 5 x repeats of the ALK motive identified by our experiments to be cleavable by the venom of Bitis sp. | Philip Hau Soerensen |
| BBa_K2355002 | Coding | This is a modification of the AmilCP part from the Uppsala 2011 team (BBa_K592009). It has a 6x His-tag attached to the C-terminal end of the protein | Philip Hau Soerensen |
| BBa_K2355006 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS | Chrysillis Magaard Polhaus |
| BBa_K2355008 | Protein-domain | This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. | Cathrine Agnete Larsen |
| BBa_K2355014 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is PEDDGYFV. | Chrysillis Magaard Polhaus |
| BBa_K2355015 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is LDEDEEDL. | Cathrine Agnete Larsen |
| BBa_K2355025 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is DEMDEKSE. | Chrysillis Magaard Polhaus |
| BBa_K2355026 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK | Cathrine Agnete Larsen |
| BBa_K2355032 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is RKRRAVLT. | Chrysillis Magaard Polhaus |
| BBa_K2355033 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is DDSDAATF. | Cathrine Agnete Larsen |
| BBa_K2355037 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV. | Chrysillis Magaard Polhaus |
| BBa_K2355039 | Protein-domain | This is a linker consisting of a peptide sequence, which has been proven experimentally to not be cleaved by the venom of Bitis gabonica, Bitis arietans and Naja nigricollis. The specific peptide sequence of the linker is IDIRGPRL. | Cathrine Agnete Larsen |
| BBa_K2355302 | Composite part | This part contains two major protein domains: A single chain avidin domain to bind the device to biotinylated surfaces, and amilCP with his-tag. Between the two domains, NgoMIV and AgeI restriction sites are present, to allow for insertion of RFC[25] part. | Cathrine Agnete Larsen |
| BBa_K2355313 | Composite part | This part is used for detection of snake venom. The Beta-galactosidase, encoded by lacZ, should allow for an decrease in detection time. | Philip Hau Soerensen |
