Cas9 & Cpf1 secretion
and activity
Difference between revisions of "Template:Team:Utrecht/MainBody"
| Line 789: | Line 789: | ||
<div id="popover-1" style="display: none;"> | <div id="popover-1" style="display: none;"> | ||
| − | + | First, gRNA needs to be added, which is complementary to the DNA sequence you want to detect. | |
<br> | <br> | ||
<br> | <br> | ||
| Line 796: | Line 796: | ||
<div id="popover-2" style="display: none;"> | <div id="popover-2" style="display: none;"> | ||
| − | + | dCas9 and dCpf1 will bind their corresponding gRNA. | |
<br> | <br> | ||
<br> | <br> | ||
| Line 803: | Line 803: | ||
<div id="popover-3" style="display: none;"> | <div id="popover-3" style="display: none;"> | ||
| − | + | DNA from the sample that matches the gRNA will first bind to one of the proteins. | |
<br> | <br> | ||
<br> | <br> | ||
| Line 810: | Line 810: | ||
<div id="popover-4" style="display: none;"> | <div id="popover-4" style="display: none;"> | ||
| − | + | Once the DNA fragment binds the other protein, the system will co-localize. This allows the protease to release the transcription factor from the complex. | |
<br> | <br> | ||
<br> | <br> | ||
| Line 898: | Line 898: | ||
var title; | var title; | ||
| − | if(i == 1) | + | /*if(i == 1) |
title = "Guide RNA"; | title = "Guide RNA"; | ||
else if(i == 2) | else if(i == 2) | ||
| Line 904: | Line 904: | ||
else if(i == 3) | else if(i == 3) | ||
title = "Signal transduction"; | title = "Signal transduction"; | ||
| + | else if(i == 4) | ||
| + | title = "Signal transduction";*/ | ||
| + | |||
| + | if(i == 1) | ||
| + | title = "Start"; | ||
| + | else if(i == 2) | ||
| + | title = "gRNA binding"; | ||
| + | else if(i == 3) | ||
| + | title = "DNA binding"; | ||
else if(i == 4) | else if(i == 4) | ||
title = "Signal transduction"; | title = "Signal transduction"; | ||
Revision as of 23:57, 1 November 2017
<!DOCTYPE html>
Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
Videos we made for the dutch public, together with 'de Kennis van Nu'.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
Read about our exchanges with other iGEM teams and government agencies.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Attributions
A thank-you for everyone that assited us, both in and outside the lab.
×
