Line 430: | Line 430: | ||
$("#tip5").css("transform","translate(0,"+((cycircle+deviceheight+3*linelength+2*circleradius)-3*heightexplanation)+"px)"); | $("#tip5").css("transform","translate(0,"+((cycircle+deviceheight+3*linelength+2*circleradius)-3*heightexplanation)+"px)"); | ||
+ | var animatinoCounter = 0; //This counter saves the number of animation that have already been triggered. If an animation is not triggering, check if the counter-count is done propertly. | ||
+ | var accu = 0; | ||
+ | |||
+ | $(window).on("scroll",checkPosition); //Checks on every scroll whether a certain animation should be triggered or not. | ||
+ | |||
+ | var finalshrink = parseInt(circleradius*0.15); //This variable is here so the shrink function doesn't have to evaluate parseIn(etc) every single time. | ||
+ | |||
+ | var triggerleftLine = parseInt(circleradius); //This variable is responsible for timely triggering the appearence of the left tips. The reason it's circleratidus is simply due to the the way x2 from the first tooltip-line is positioned (circleradius left from canvas start). | ||
+ | |||
+ | var startMovement = false; //This variable is the responsible for triggering the first moveCircleY. It's declared here so it is within scoope of all functions. | ||
+ | |||
+ | var triggerrightLine = parseInt(canvaswidth*0.5-circleradius); //This variable is responsible for timely triggering the appearence of right tips. The reason it's canvaswidth*etc is simply due to the way the intended lenght of the line was calculated. | ||
+ | |||
+ | var endstep1 = parseInt(cycircle+linelength+circleradius+0.1*deviceheight); //This variable tells the first set of animations where they should end. | ||
+ | var endstep2 = parseInt(endstep1+0.25*deviceheight); //This variable tells the second set of animations where they should end. | ||
+ | var endstep3 = parseInt(endstep2+0.30*deviceheight); | ||
+ | |||
+ | var endstep4 = parseInt(endstep3+0.25*deviceheight); | ||
+ | |||
+ | var endstep5 = parseInt(endstep4 +linelength +0.1*deviceheight); | ||
+ | |||
+ | var endstep6 = parseInt(endstep5 + linelength + circleradius); | ||
+ | |||
+ | |||
+ | alert("adios"); | ||
</script> | </script> | ||
</html> | </html> |
Revision as of 08:18, 8 July 2017
CascAID stands for Cas13a Controlled Assay for Infectious Diseases. Our goal this year is to create a novel paper-based microfluidic device for the detection of specific RNA sequences.
Nowadays, there is a trend among medical practitioners to prescribe antibiotics when bacterial infections are suspected without a laboratory confirmation as a way for speeding up recovery. This has led to an increase in antibiotic-resistant bacteria that makes it difficult to treat infected patients.
Nucleic acid-based detection methods could allow for faster diagnosis. Therefore, we seek to develop a method that allows to report the presence of a specific pathogen within hours. For this reason, we will use the CRISPR effector Cas13a, which is able to target specific single-stranded RNA. This system allows for the simple and fast design of new sequence targets, being an ideal tool for detecting fast mutating pathogens. In the long term, we see our device as an easy-to-use and fast diagnostic tool in developing countries as well as an instrument in developed countries for discerning between bacterial and viral infections, that could help reducing antibiotics prescription.
Step 1: A sample, such as blood or saliva, is placed into the device.
Step 3: Cas13a binds the target sequence and cuts it in smaller fragments. After this inital digestion, the enzyme changes into a RNAase-active conformation.
Step 5: An easy to interpret read-out tells whether a specific pathogen was present in the sample.
Step 2: RNA is purified with the help of our reusable, 3D-printed microfluidic device.
Step 4: While in this conformation, Cas13a degrades the read-out RNA, producing a visible signal.