Results
Line 108: | Line 108: | ||
<center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/e/e7/T--IISER-Mohali-INDIA--results4.jpg" alt="Flow chart"></td></tr></table></center> | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/e/e7/T--IISER-Mohali-INDIA--results4.jpg" alt="Flow chart"></td></tr></table></center> | ||
<h4>With higher concentrations of salicylate, the response was as quick as before, but with greater magnitude. The saturation of the fluorescence signal could not be studied with higher concentrations as the fluorescence intensity crossed the upper-limit of the instrument. Thus, we observe that the <i>mar</i> promoter responds within 20 minutes of adding salicylate.</h4> | <h4>With higher concentrations of salicylate, the response was as quick as before, but with greater magnitude. The saturation of the fluorescence signal could not be studied with higher concentrations as the fluorescence intensity crossed the upper-limit of the instrument. Thus, we observe that the <i>mar</i> promoter responds within 20 minutes of adding salicylate.</h4> | ||
− | <h3>Cloning of chromoprotein for selection and part submission for registry :</h3> | + | <h3>Cloning of chromoprotein for selection and part submission for registry:</h3> |
− | <h4>We selected five different chromoproteins | + | <h4>We selected five different chromoproteins to study their kinetics and to evaluate their utility in our circuit.</h4> |
− | <h4>We have observed that out of these five different chromoproteins, chromoprotein sequence | + | <h4>We have observed that out of these five different chromoproteins, chromoprotein sequence numbers 1-3 developed color relatively fast (24-30 hours) but chromoprotein sequence numbers 4-5 developed color relatively slow. Hence, 4 and 5 are not suitable for our circuit. |
− | Therefore, we selected | + | Therefore, we selected chromoproteins: amilCP Blue chromoprotein and fwYellow chromoprotein for use in our circuit. |
− | Further all these chromoproteins were cloned with RBS and LacI promoter by 3A assembly and submitted in depository | + | Further, all these chromoproteins were cloned with RBS and LacI promoter by 3A assembly and submitted in the depository (accession number from BBa_K2320001- K2320005). |
</h4> | </h4> | ||
<center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/a/a4/T--IISER-Mohali-INDIA--dish.jpg" alt="Dishes"></td></tr></table></center> | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/a/a4/T--IISER-Mohali-INDIA--dish.jpg" alt="Dishes"></td></tr></table></center> |
Revision as of 00:09, 2 November 2017