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− | {{Heidelberg/partspanelleft|{{#tag:html|<a href="http://parts.igem.org/Part:BBa_K2398000">BBa_K2398000</a>}}|We present as best basic part the codon-optimized version of the cytochrome c protein derived from <i>{{#tag:html|<a href="http://www.rcsb.org/pdb/explore.do?structureId=3cp5">Rhodotermus marinus</a>}}</i> that is able to convert silicon educts to organosilicon products.|https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png| | }} | + | {{Heidelberg/partspanelleft|{{#tag:html|<a href="http://parts.igem.org/Part:BBa_K2398000">BBa_K2398000</a>}}|We present as best basic part the codon-optimized version of the cytochrome c protein derived from <i>{{#tag:html|<a href="http://www.rcsb.org/pdb/explore.do?structureId=3cp5">Rhodotermus marinus</a>}}</i> that is able to convert silicon educts to organosilicon products.|https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png| |}} |
This cytochrome c variant provides an easy to use tool that is accessible to everyone in the synthetic biology community and allows the user to harness the vast potential of organosilicons. | This cytochrome c variant provides an easy to use tool that is accessible to everyone in the synthetic biology community and allows the user to harness the vast potential of organosilicons. | ||
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− | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png|Figure 1:|Compounds used in the project of organosilicons and its respective products.|}} | + | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png|Figure 1:|Compounds used in the project of organosilicons and its respective products.|style = float:none}} |
First, we tested the enzyme on the conversion of the compound (1) with (3) to the final product of (5) (Fig. 1). The gas chromatogram shows one product peak which emerges at 9.2 min retention time. Analysis of the gas chromatogram indicates a conversion rate of >99% on the basis of the non-existence of the respective educt peak. | First, we tested the enzyme on the conversion of the compound (1) with (3) to the final product of (5) (Fig. 1). The gas chromatogram shows one product peak which emerges at 9.2 min retention time. Analysis of the gas chromatogram indicates a conversion rate of >99% on the basis of the non-existence of the respective educt peak. |
Revision as of 00:42, 2 November 2017
BBa_K2398000
We present as best basic part the codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus that is able to convert silicon educts to organosilicon products.![Circularization Construct](https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png)
![](https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png)
Figure 1:
Compounds used in the project of organosilicons and its respective products.
![](https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png)
Figure 2:
Gas chromatogram for the reaction of educt (1) and (3) to the product (5). 11.7 minutes retention time indicates product formation. Unconverted educts converge 6.9 and 7.2 (7.4) minutes.
![](https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png)
Figure 3:
Mass chromatogram shows the breakdown of the product (5). The product itself corresponds to a mass of 251 daltons.
![](https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png)
Figure 4:
Gas chromatogram for the reaction of educt (2) and (3) to the product (4). 9.2 minutes retention time indicates product formation.
![](https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png)
Figure 5:
Mass chromatogram shows the breakdown of the product (4). The product itself corresponds to a mass of 236 daltons.