Difference between revisions of "2017.igem.org/Team:BIT/Track"

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 <section id="works">
-   <div class="container-fluid" style="text-align: center"><img src="https://static.igem.org/mediawiki/2017/c/c0/BIT_figure_TrackTitle.jpeg" alt=""></div>
+   <div class="container-fluid" style="text-align: center"><div class="container"><img src="https://static.igem.org/mediawiki/2017/c/c0/BIT_figure_TrackTitle.jpeg" alt=""></div></div>
 <div class="container" style="text-align: center; padding-top: 20px">
    <div style="text-align: center"> <a class="largetitle"> Stuff here are Solid #dataspeaks </a> </div>
    <div class="container">
        <div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Biosensing Group </a> </div>
-      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 50px">1. Overview</a> </div>
-      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 50px">2. The specific binding of AFP to AP-273</a> </div>
-      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 50px">3. EDC Plus Sulfo-NHS</a> </div>
-      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 50px">4. Linear Relationship between AFP and Lysine</a> </div>
-      <div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Genetic Expression </a> </div>
        <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">1. Overview</a> </div>
-       <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. Medium Preparation</a> </div>
+       <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. The specific binding of AFP to AP-273</a> </div>
-       <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. Plasmid Transformation and Cultivating: To amplify the plasmids.</a> </div>
+       <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. EDC Plus Sulfo-NHS</a> </div>
-       <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">4. Mini Plasmid: To extract plasmids from bacterium.</a> </div>
+       <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">4. Linear Relationship between AFP and Lysine</a> </div>
+      <div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Genetic Engineering </a> </div>
        <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">1. Overview</a> </div>
        <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. Medium Preparation</a> </div>
        <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. Plasmid Transformation and Cultivating: To amplify the plasmids.</a> </div>
        <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">4. Mini Plasmid: To extract plasmids from bacterium.</a> </div>
+      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">5.	Digestion: To get the target gene and the vector.</a> </div>
+      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">6.	Enzyme Linked: To link the target gene to the vector.</a> </div>
+      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">7. Electrophoresis.</a> </div>
+	  <div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Enzyme Digestion and Processing </a> </div>
+      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">1.	Electroporation</a> </div>
+      <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. Knock-Out of LysA Gene in E.coli BW25113 by λRed Recombination System</a> </div>
+<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. The Preparation of Targeting Vectorm</a> </div>
+	  <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">4. The Transformation of Plasmid pKD46</a> </div>
+	  <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">5. The Verification of Strain</a> </div>
+	  <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">A. Antibiotics Preparation</a> </div>
+	   <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">B. Competent Cell Preparation</a> </div>
+	   <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">C. Submission DNA Preparation</a> </div>
+	   <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">D. TIANamp Bacteria DNA Kit</a> </div>
    </div>
 </div>