Difference between revisions of "Team:Tianjin/Design"

Line 239: Line 239:
 
       <h4>OVERVIEW </h4>
 
       <h4>OVERVIEW </h4>
 
               <hr>
 
               <hr>
       <p>After we found there might be revolutionary usage about the mating type switch (<b>MTS</b>) of yeasts in our heavy metal deposition system, In laboratory, the species of budding yeast we usually used are <i>BY4741</i> and <i>BY4742</i>, whose <i>HO</i> gene are knocked out. Therefore, we intended to use two groups of <i>MATa</i> yeasts to realize the mating switcher. </p>
+
       <p> We found there might be revolutionary usage about the mating type switch (<b>MTS</b>) of yeasts in our heavy metal deposition system. Whereas, in laboratory, the species of budding yeast we usually use are <i>BY4741</i> and <i>BY4742</i>, whose <i>HO</i> gene are knocked out. Therefore, we intended to use two groups of <i>MATa</i> yeasts to realize the mating switcher by a specially designed system. </p>
<p>One of these groups was required to achieve <b>MTS</b>. We decided to achieve <b>MTS</b> by introducing the <i>HO</i> gene into this group of yeasts— Saccharomyces cerevisiae (<i>BY4741</i>, in our lab, whose chromosome Ⅹhas been switched by synthetic chromosome Ⅹ. And it has been renamed as <i>SynⅩ</i> similarly hereinafter). To make the <b>MTS</b> controllable, it is necessary for us to adopt inducible promoters to initiate the expression of <i>HO</i> gene or create a pathway functioning as single gene regulator. Eventually, we landed on the <i>Gal1</i> promoter first, for its convenience and efficiency. As we read in R. Scott McIsaac’s work, their rapid, tunable, single-gene specificity control system of single gene in yeasts has given us much impression. Therefore, we decided to use this system as one of our pathway designs for the expression of <i>HO</i> gene. </p>
+
<p>One of these groups was required to achieve <b>MTS</b>. We decided to achieve <b>MTS</b> by introducing the <i>HO</i> gene into this group of yeasts— Saccharomyces cerevisiae (<i>BY4741</i>, in our lab, whose chromosome Ⅹ has been switched by synthetic chromosome Ⅹ. And it has been renamed as <i>SynⅩ</i> similarly hereinafter). To make the <b>MTS</b> controllable, it was necessary for us to adopt inducible promoters to initiate the expression of <i>HO</i> gene or create a pathway functioning as single gene regulator. Eventually, we landed on the <i>Gal1</i> promoter first, for its convenience and efficiency. As we read in R. Scott McIsaac’s work, their rapid, tunable, single-gene specificity control system of single gene in yeasts has given us much impression. Therefore, we decided to use this system as one of our pathway designs for the expression of <i>HO</i> gene. </p>
 
                     <h4>GETTING THE CHASSIS </h4>
 
                     <h4>GETTING THE CHASSIS </h4>
 
                     <hr>  
 
                     <hr>  

Revision as of 01:42, 2 November 2017

/* OVERRIDE IGEM SETTINGS */

Design


Background

Human existence on earth is almost impossible without the heavy metals. Even though important to mankind, exposure to them during production, usage and their uncontrolled discharge into the environment has caused lots of hazards to man, other organisms and the environment itself. Heavy metals can enter human tissues and organs via inhalation, diet, and manual handling. As the process of urbanization and industrialization goes deeper and deeper, heavy metal pollution, a noticeable threaten to almost all the creatures, has become an essential problem to solve.

According to our human practice, the situation of heavy metal pollution (copper and cadmium ions) is marked on a world map, and the severity of heavy metal pollution has been increasing all over this map. Places with serious pollution include middle Asia, eastern Asia, southern Europe, and Latin America. In addition, not only fresh water source, but also soil and crops are seriously contaminated by heavy metals. On average, during three out of ten suppers we have, we absorb excess heavy metals over the standard concentration.

Considering the rigorous situation we face, our team decided to design an advanced system for typical toxic heavy metal disposal based on Saccharomyces cerevisiae.