Line 58: | Line 58: | ||
<div class="slide-right-content slide-right-content-1 is-current"> | <div class="slide-right-content slide-right-content-1 is-current"> | ||
<h1>Determining the plasmid copy number</h1> | <h1>Determining the plasmid copy number</h1> | ||
− | <div class="category">Design</div> | + | <div class="category">Design and Results</div> |
<p>Preparing for the framework: standard curve generation and plasmid copy number evaluation</p> | <p>Preparing for the framework: standard curve generation and plasmid copy number evaluation</p> | ||
<div class="readmore" data-modal="1">read more</div> | <div class="readmore" data-modal="1">read more</div> | ||
Line 64: | Line 64: | ||
<div class="slide-right-content slide-right-content-2 hidden"> | <div class="slide-right-content slide-right-content-2 hidden"> | ||
<h1>Plasmid copy number control</h1> | <h1>Plasmid copy number control</h1> | ||
− | <div class="category">Design</div> | + | <div class="category">Design and Results</div> |
<p>Flexible copy number control is the core of our framework, which is based on re-engineered ColE1 origin of replicon.</p> | <p>Flexible copy number control is the core of our framework, which is based on re-engineered ColE1 origin of replicon.</p> | ||
<div class="readmore" data-modal="2">read more</div> | <div class="readmore" data-modal="2">read more</div> | ||
Line 70: | Line 70: | ||
<div class="slide-right-content slide-right-content-3 hidden"> | <div class="slide-right-content slide-right-content-3 hidden"> | ||
<h1>Multiple plasmid groups</h1> | <h1>Multiple plasmid groups</h1> | ||
− | <div class="category">Design</div> | + | <div class="category">Design and Results</div> |
<p>Multi-plasmid framework would not be much without multiple plasmids. We have equiped our synthetic origin of replication with specific recognition sequences to create unique plasmid groups.</p> | <p>Multi-plasmid framework would not be much without multiple plasmids. We have equiped our synthetic origin of replication with specific recognition sequences to create unique plasmid groups.</p> | ||
<div class="readmore" data-modal="3">read more</div> | <div class="readmore" data-modal="3">read more</div> | ||
Line 76: | Line 76: | ||
<div class="slide-right-content slide-right-content-4 hidden"> | <div class="slide-right-content slide-right-content-4 hidden"> | ||
<h1>Global copy number regulation</h1> | <h1>Global copy number regulation</h1> | ||
− | <div class="category">Design</div> | + | <div class="category">Design and Results</div> |
− | <p> | + | <p>We created a universal copy number control mechanism for all plasmid groups at once. For this we have applied the effects of the Rop protein, which increases the binding affinity of RNA I and RNA II, hence - inhibition of replication</p> |
<div class="readmore" data-modal="4">read more</div> | <div class="readmore" data-modal="4">read more</div> | ||
</div> | </div> | ||
<div class="slide-right-content slide-right-content-5 hidden"> | <div class="slide-right-content slide-right-content-5 hidden"> | ||
<h1>SynORI selection system</h1> | <h1>SynORI selection system</h1> | ||
− | <div class="category">Design</div> | + | <div class="category">Design and Results</div> |
<p>Having multiple plasmids in a cell means using multiple antibiotics. Or does it?</p> | <p>Having multiple plasmids in a cell means using multiple antibiotics. Or does it?</p> | ||
<div class="readmore" data-modal="5">read more</div> | <div class="readmore" data-modal="5">read more</div> | ||
Line 88: | Line 88: | ||
<div class="slide-right-content slide-right-content-6 hidden"> | <div class="slide-right-content slide-right-content-6 hidden"> | ||
<h1>Active partitioning system</h1> | <h1>Active partitioning system</h1> | ||
− | <div class="category">Design</div> | + | <div class="category">Design and Results</div> |
<p>If at least one of the plasmid group has a low copy number, they require extra care to not be lost at cell division. Therefore, SynORI framework incorporates a <i>special</i> partitioning system derived from pSC101 replicon. </p> | <p>If at least one of the plasmid group has a low copy number, they require extra care to not be lost at cell division. Therefore, SynORI framework incorporates a <i>special</i> partitioning system derived from pSC101 replicon. </p> | ||
<div class="readmore" data-modal="6">read more</div> | <div class="readmore" data-modal="6">read more</div> |
Revision as of 01:37, 2 November 2017
![](https://static.igem.org/mediawiki/2017/3/39/T--Vilnius-Lithuania--logoleft.png)
![](https://static.igem.org/mediawiki/2017/0/08/T--Vilnius-Lithuania--logoleft-dark.png)
![](https://static.igem.org/mediawiki/2017/4/4b/T--Vilnius-Lithuania--logoright.png)
![](https://static.igem.org/mediawiki/2017/2/21/T--Vilnius-Lithuania--logoright-dark.png)
![](https://static.igem.org/mediawiki/2017/f/f9/T--Vilnius-Lithuania--arrows.png)
use keyboard, swipe or scroll