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Revision as of 01:34, 2 November 2017

IGEM BIT HOMEPAGE

Biosensing Group
1. Overview
2. The specific binding of AFP to AP-273
3. EDC Plus Sulfo-NHS
4. Linear Relationship between AFP and Lysine
Genetic Engineering
1. Overview
2. Medium Preparation
3. Plasmid Transformation and Cultivating: To amplify the plasmids.
4. Mini Plasmid: To extract plasmids from bacterium.
5. Digestion: To get the target gene and the vector.
6. Enzyme Linked: To link the target gene to the vector.
7. Electrophoresis.
Enzyme Digestion and Processing
1. Electroporation
2. Knock-Out of LysA Gene in E.coli BW25113 by λRed Recombination System
3. The Preparation of Targeting Vectorm
4. The Transformation of Plasmid pKD46
5. The Verification of Strain
A. Antibiotics Preparation
B. Competent Cell Preparation
C. Submission DNA Preparation
D. TIANamp Bacteria DNA Kit