Line 55: | Line 55: | ||
<ul> | <ul> | ||
<li>Team discussion, planning and fundraising</li> | <li>Team discussion, planning and fundraising</li> | ||
− | <li>Counting colonies of Transformants for the Interlab Challenge: | + | <li>Counting colonies of Transformants for the Interlab Challenge:</li> |
<ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul> | <ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/1/17/T--BOKU-Vienna--Notebook_countedplate1.jpg" alt="Transformants on plate" height="200"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f6/T--BOKU-Vienna--Notebook_countedplate2.jpg" alt="Transformants on plate" height="200"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/2c/T--BOKU-Vienna--Notebook_countedplate3.jpg" alt="Transformants on plate" height="200"> | ||
<li>Miniprep of 8 plasmids for Golden Gate Standard - according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#miniprep" target="_blank">protocol</a></li> | <li>Miniprep of 8 plasmids for Golden Gate Standard - according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#miniprep" target="_blank">protocol</a></li> | ||
<ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul> | <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul> |
Revision as of 15:29, 10 July 2017
Monday, July 3rd.
- Team discussion and overview:
- Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
- Planning of the procedures including primer design
- Preparing the growth media and autoclaving
- LB medium preparation (4L): Mina, Michael
- Autoclaving: Mina, Rebecca
Tuesday, July 4th.
Wednesday, July 5th.
- Planning and fundraising:
- Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
- Aliquoting 1mL LB into Eppendorf tubes
- Lisa, Josef
- Transferring Backbones onto agar plates:
- Julian
- Interlab challenge - transformation: According to protocol:
- 8 transformations - from Kit Plate 6 InterLab
- 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
- stored at 35°C
- Lisa, Mathias, Michael, Mina, Rebecca
Thursday, July 6th.
- Team discussion, planning and fundraising
- Counting colonies of Transformants for the Interlab Challenge:
- Colonies 200µL: 77, 20µL: 26, 100µL: 43
- Miniprep of 8 plasmids for Golden Gate Standard - according to protocol
- Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
- Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]
Friday, July 7th.
- Team discussion, Planning and Fundraising
- Interlab Challenge: Fluorescein fluorescence standard curve
- Christina