Difference between revisions of "Team:XJTLU-CHINA/Demonstrate"
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</figure> | </figure> | ||
<figcaption> | <figcaption> | ||
| − | <strong>Fig. 2 time gradients for dot | + | <strong>Fig. 2 time gradients for dot blot. The left graph shows the protein were produced and right is non-induced (negative control). The result shows that whole circuit can be induced by IPTG |
</strong> | </strong> | ||
</figcaption> | </figcaption> | ||
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<div class="para"> | <div class="para"> | ||
<h2>3. Inhibition Ring</h2> | <h2>3. Inhibition Ring</h2> | ||
| − | <p style="font-size:20px">This experiment qualitatively evaluate the | + | <p style="font-size:20px">This experiment was to qualitatively evaluate the effectivity of Anti-microbial peptides (AMPs). The result showed that the AMPs have effectivity on <i>Staphylococcus aureus. </i></p> |
</div> | </div> | ||
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<figcaption><strong>D</strong></figcaption> | <figcaption><strong>D</strong></figcaption> | ||
</figure> | </figure> | ||
| − | <figcaption><strong>Fig. 3: Inhibition Ring of anti-microbial peptides on Staphylococcus aureus. The concentration of peptides is 1mg/ml and volume is 20ul. (A) LL-37 (B) Grammistin-Pp1 (C) GF-17 (D) three peptides (LL-37, Grammistin-Pp1, and GF-17) mix | + | <figcaption><strong>Fig. 3: Inhibition Ring of anti-microbial peptides on Staphylococcus aureus. The concentration of peptides is 1mg/ml and volume is 20ul. (A) LL-37 (B) Grammistin-Pp1 (C) GF-17 (D) three peptides (LL-37, Grammistin-Pp1, and GF-17) mix.</strong></figcaption> |
</div> | </div> | ||
<div class="para"> | <div class="para"> | ||
| − | <p style="font-size:20px">Fig. 3 shows the AMPs | + | <p style="font-size:20px">Fig. 3 shows the AMPs have effectivity on <i>Staphylococcus aureus</i>.</p> |
</div> | </div> | ||
<div class="container"> | <div class="container"> | ||
<div class="para"> | <div class="para"> | ||
<p style="font-size:30px"> | <p style="font-size:30px"> | ||
| − | Aimed at examining the efficacy of the antimicrobial peptides on killing S. aureus | + | Aimed at examining the efficacy of the antimicrobial peptides on killing S. aureus each experiment below were repeated 3 |
| − | times | + | times. |
</p> | </p> | ||
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<div class="para"> | <div class="para"> | ||
<p style="font-size:20px"> | <p style="font-size:20px"> | ||
| − | Both single peptide of the three AMPs and the mixture of them | + | Both single peptide of the three AMPs and the mixture of them had conspicuous lethal effect on S. aureus. |
</div> | </div> | ||
Revision as of 02:34, 2 November 2017
Demonstration
For the whole circuit construct, we did following experiments:
1. Gel electrophoresis
2. Expression of LL-37 in bacteria screened by dot blotting
3. Inhibition Ring
This experiment was to qualitatively evaluate the effectivity of Anti-microbial peptides (AMPs). The result showed that the AMPs have effectivity on Staphylococcus aureus.
Fig. 3 shows the AMPs have effectivity on Staphylococcus aureus.
Aimed at examining the efficacy of the antimicrobial peptides on killing S. aureus each experiment below were repeated 3 times.
4. Determination of the minimal inhibitory concentration (MIC) of the three AMPs.
Both single peptide of the three AMPs and the mixture of them had conspicuous lethal effect on S. aureus.
5. Absorbance (cell density) of the biofilm of S. aureus against different concentrations of the three individual AMPs or a mixture of them
The biofilm formation of S. aureus was to a great extent inhibited by these three AMPs.
Experience and conclusions:
- Peptides has shown their antimicrobial activity on S. aureus. And they can successfully expressed in E. coli.
- AgrA, which is an essential protein in quorum sensing construct, signifies toxicity to cells during growth.
- We have trouble in obtaining enough plasmid DNA extracted from L. lactis after transformation, probably due to either low copy number or low yield from the extraction protocol.
Collaborators and Supporters
