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<div class="column full_size"> | <div class="column full_size"> | ||
<p style="font-family: quicksand;font-size:150%;">RNA thermodynamic Modelling</p> | <p style="font-family: quicksand;font-size:150%;">RNA thermodynamic Modelling</p> | ||
− | <p style="font-family: roboto;font-size:125%; | + | <p style="font-family: roboto;font-size:125%;color:blue"><b>Assumption 1: Switch MFE (Minimum Free Energy) correlates with the expression leakage</p></b> |
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
● Switch MFE is the minimum Gibbs free energy that a toehold switch could have among all the possible structures.<br> | ● Switch MFE is the minimum Gibbs free energy that a toehold switch could have among all the possible structures.<br> | ||
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</p> | </p> | ||
<br> | <br> | ||
− | <u><b><p style="font-family: roboto;font-size:125%;">Assumption 2: ΔG< | + | <u><b><p style="font-family: roboto;font-size:125%;">Assumption 2: ΔG<sub>RBS-Linker</sub> correlates with the duplex expression</p></b></u> |
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
● ΔG<sub>RBS-Linker</sub> is the Gibbs free energy of the RNA sequence starting from the RBS to the linker in the switch-trigger duplex.<br> | ● ΔG<sub>RBS-Linker</sub> is the Gibbs free energy of the RNA sequence starting from the RBS to the linker in the switch-trigger duplex.<br> | ||
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<br> | <br> | ||
− | <p style="font-family: roboto;font-size:115%;"> To improve <a href="https://2015.igem.org/Team:CGU_Taiwan">Chang Gung University(CGU)’s oral cancer toehold switch</a>, we employed the screening function of our program. A toehold switch was chosen that is predicted to outperform the CGU’s switch according to the MFE RBS-Linker.</p> | + | <p style="font-family: roboto;font-size:115%;"> To improve <a href="https://2015.igem.org/Team:CGU_Taiwan/Results">Chang Gung University(CGU)’s oral cancer toehold switch</a>, we employed the screening function of our program. A toehold switch was chosen that is predicted to outperform the CGU’s switch according to the MFE RBS-Linker.</p> |
Revision as of 02:23, 2 November 2017