Difference between revisions of "Team:Cadets2Vets/Experiments"

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{{Team:Cadets2Vets/SideMenu}}
 
{{Team:Cadets2Vets/SideMenu}}
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<html>
 
  
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<head>
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<html xmlns="http://www.w3.org/1999/xhtml">
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<head>
 
<title>
 
<title>
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EXPERIMENTS - USE THIS ONE!!!</title>
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Experiments - Home</title>
 
<!-- META -->
 
<!-- META -->
 
<meta charset="UTF-8">
 
<meta charset="UTF-8">
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<script src="https://www.youtube.com/iframe_api"></script>
 
<script src="https://www.youtube.com/iframe_api"></script>
 
<!-- CSS -->
 
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<link rel="stylesheet" href="//edit.www.imcreator.com/css/common.css?v=1.4.0p1">
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<link rel="stylesheet" href="//xprs.imcreator.com/css/common.css?v=1.4.1b">
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<link rel="stylesheet" href="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.css" />
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<link rel="stylesheet" href="//xprs.imcreator.com/js/lib/sweetalert/sweetalert.css" />
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/all_css.css?v=1.4.0p1" />
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<link rel="stylesheet" type="text/css" href="//xprs.imcreator.com/all_css.css?v=1.4.1b" />
  
 
 
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<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 
<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 
 
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/fonts.css?v=1.4.0p1" />
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<link rel="stylesheet" type="text/css" href="//xprs.imcreator.com/css/fonts.css?v=1.4.1b" />
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/effects.css?v=1.4.0p1" />
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<link rel="stylesheet" type="text/css" href="//xprs.imcreator.com/css/effects.css?v=1.4.1b" />
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/lightbox.css?v=1.4.0p1">
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<link rel="stylesheet" type="text/css" href="//xprs.imcreator.com/css/lightbox.css?v=1.4.1b">
 
 
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/spimeview.css?v=1.4.0p1">
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<link rel="stylesheet" type="text/css" href="//xprs.imcreator.com/css/spimeview.css?v=1.4.1b">
 
 
 
 
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<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
 
<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
 
 
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<script src="//edit.www.imcreator.com/js/xprs_helper.js?v=1.4.0p1" type="text/javascript"></script>
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<script src="//xprs.imcreator.com/js/xprs_helper.js?v=1.4.1b" type="text/javascript"></script>
 
 
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<script src="//edit.www.imcreator.com/js/lib/jquery.cookie.min.js" type="text/javascript"></script>
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<script src="//xprs.imcreator.com/js/lib/jquery.cookie.min.js" type="text/javascript"></script>
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<script src="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.min.js" type="text/javascript"></script>
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<script src="//xprs.imcreator.com/js/lib/sweetalert/sweetalert.min.js" type="text/javascript"></script>
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<script src="//edit.www.imcreator.com/translation_js?lang=en" type="text/javascript"></script>
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<script src="//xprs.imcreator.com/translation_js?lang=en" type="text/javascript"></script>
 
 
 
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<!-- User Head Code -->
 
 
 
 
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<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.0p1" type="text/javascript"></script>
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<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.1b" type="text/javascript"></script>
  
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<script type="text/javascript" src="//edit.www.imcreator.com/all_js.js?v=1.4.0p1"></script>
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<script type="text/javascript" src="//xprs.imcreator.com/all_js.js?v=1.4.1b"></script>
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<script src="//edit.www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
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<script src="//xprs.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
 
<script type="text/javascript">IMOS.pageView();</script>
 
<script type="text/javascript">IMOS.pageView();</script>
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<script>
 
<script>
 
 
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+
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</script></head>
 
</script></head>
  
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<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.0p1" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//edit.www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
+
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1b" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//xprs.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 
 
−
<div id="xprs" data-website-name="USE THIS ONE!!!" class="xprs-holder" >
+
<div id="xprs" data-website-name="Home" class="xprs-holder" >
 
<div class="main-page "  >
 
<div class="main-page "  >
 
<div id="content">
 
<div id="content">
−
<div  id="vbid-59a5b-oiquicn3"  class="master container style-59a5b-n6hlc3qt content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="EXPERIMENTS"  data-itemslug="experiments" data-itemstyleid="style-59a5b-n6hlc3qt" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-59a5b-oiquicn3" data-preset-type-id="ITEMS" data-preview-style="style-59a5b-n6hlc3qt" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="http://edit.www.imcreator.com/viewer" >
+
<div  id="vbid-ab048c8e-h2ujhr1w"  class="master container style-ab048c8e-ulmcgqel content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Experiments"  data-itemslug="experiments" data-itemstyleid="style-ab048c8e-ulmcgqel" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-ab048c8e-h2ujhr1w" data-preset-type-id="ITEMS" data-preview-style="style-ab048c8e-ulmcgqel" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="https://xprs.imcreator.com/viewer" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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<!-- TITLE TEMPLATE -->
 
<!-- TITLE TEMPLATE -->
 
 
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<div class="preview-title-holder removable-parent">
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<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
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+
−
<h2 id="vbid-59a5b-0dca3gi2" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Menu</h2>
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−
+
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</div>
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<br />
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<!-- SUBTITLE TEMPLATE -->
 
<!-- SUBTITLE TEMPLATE -->
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-qpimjeag" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-466a74f8-j2jzxizg" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-w5gehayb"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team Members</span>
+
<span id="b951bd16"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-l5xeq0z6" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-5515b55f-jgjhhkan" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-9i6v7wly"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Projects</span>
+
<span id="928ba103"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 
</a>
 
</a>
 
 
Line 183: Line 179:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xtfijafb" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-9e25704e-ynxs9cit" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-b9vovddz"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
+
<span id="e08666cf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook-July</span>
 
</a>
 
</a>
 
 
Line 194: Line 190:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-bb9vg46d" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xtfijafb" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-hlhlmo7j"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
+
<span id="bd841f1b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 
</a>
 
</a>
 
 
Line 205: Line 201:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-hjemejig" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-04d15a56-3o81aqko" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-k2taajbu"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Human Practices</span>
+
<span id="a6cf4805"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
 
</a>
 
</a>
 
 
Line 216: Line 212:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-0rawt2n3" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-4f279020-pcxhuaas" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-dv55z3u4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Awards</span>
+
<span id="element-077b707f6118c36"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
 
</a>
 
</a>
 
 
Line 227: Line 223:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-szkh4ovx" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-51653ffc-lok1anrm" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-qli0rtxn"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Judging Form</span>
+
<span id="2ed4772d"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook-July</span>
 
</a>
 
</a>
 
 
Line 238: Line 234:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-fed51ac9-l8qwmrzh" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-egwntcng"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
+
<span id="ece571a0"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 
</a>
 
</a>
 
 
Line 249: Line 245:
 
<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-akva45w2" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="https://xprs.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-ab048c8e-h2ujhr1w" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-vcbsdeug"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
+
<span id="463d44e3"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
−
</a>
+
−
+
−
</li>
+
−
 
+
−
+
−
+
−
<li class="removable-parent">
+
−
+
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-oiquicn3" data-link-type="EXISTING"  target="_self" >
+
−
+
−
<span id="vbid-59a5b-bnaeflqb"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
+
−
</a>
+
−
+
−
</li>
+
−
 
+
−
+
−
+
−
<li class="removable-parent">
+
−
+
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-kmybr6mb" data-link-type="EXISTING"  target="_self" >
+
−
+
−
<span id="vbid-59a5b-c9f8qbif"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
+
−
</a>
+
−
+
−
</li>
+
−
 
+
−
+
−
+
−
<li class="removable-parent">
+
−
+
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xghz2dmu" data-link-type="EXISTING"  target="_self" >
+
−
+
−
<span id="vbid-59a5b-cxv3wrcd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
+
−
</a>
+
−
+
−
</li>
+
−
 
+
−
+
−
+
−
<li class="removable-parent">
+
−
+
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-26336972-lok1anrm" data-link-type="EXISTING"  target="_self" >
+
−
+
−
<span id="1a721f1e"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
+
 
</a>
 
</a>
 
 
Line 337: Line 289:
 
 
 
<!-- PROMO START -->
 
<!-- PROMO START -->
−
<div  id="vbid-59a5b-joav3wvi"  class="master item-box  gallery-box style-59a5b-kakb45uo         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-kakb45uo" data-preview-styleid='style-59a5b-kakb45uo' data-preset-type-id="PROMO">
+
<div  id="vbid-ab048c8e-io0lb3dw"  class="master item-box  gallery-box style-ab048c8e-wg9dtpiq         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-ab048c8e-wg9dtpiq" data-preview-styleid='style-ab048c8e-wg9dtpiq' data-preset-type-id="PROMO">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 
 
−
<!-- CACHED VERSION (7) OF vbid-59a5b-joav3wvi FROM  22-06-2017 20:27:27 --><div  class="sub container style-59a5b-kakb45uo content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-kakb45uo" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-joav3wvi" data-preset-type-id="PROMO" data-preview-style="style-59a5b-kakb45uo" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path="http://edit.www.imcreator.com/viewer" >
+
<!-- CACHED VERSION (7) OF vbid-ab048c8e-io0lb3dw FROM  31-07-2017 23:27:11 --><div  class="sub container style-ab048c8e-wg9dtpiq content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-ab048c8e-wg9dtpiq" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-ab048c8e-io0lb3dw" data-preset-type-id="PROMO" data-preview-style="style-ab048c8e-wg9dtpiq" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path="https://xprs.imcreator.com/viewer" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
Line 366: Line 318:
 
 
 
 
−
<div  id="vbid-59a5b-7axmunec"  class="sub item-box  page-box style-59a5b-kakb45uo         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-kakb45uo" data-preview-styleid='style-59a5b-kakb45uo' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-ab048c8e-psahexv7"  class="sub item-box  page-box style-ab048c8e-wg9dtpiq         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ab048c8e-wg9dtpiq" data-preview-styleid='style-ab048c8e-wg9dtpiq' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-7axmunec" data-preview-style="style-59a5b-kakb45uo" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="504" data-orig-thumb-width="600" data-vbid="vbid-59a5b-7axmunec" data-bgimg="https://lh3.googleusercontent.com/eWaGqkBdwsI8pTfu-rzx3x3--TOAN6NYqmRcGlI9_shvW82ZIctRd_ZA0f0wNG1JHFPzJZsI9CpGY44PNEQ">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-ab048c8e-psahexv7" data-preview-style="style-ab048c8e-wg9dtpiq" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="504" data-orig-thumb-width="600" data-vbid="vbid-ab048c8e-psahexv7" data-bgimg="https://lh3.googleusercontent.com/eWaGqkBdwsI8pTfu-rzx3x3--TOAN6NYqmRcGlI9_shvW82ZIctRd_ZA0f0wNG1JHFPzJZsI9CpGY44PNEQ">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
Line 380: Line 332:
 
<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
<div class="vertical-aligner">
−
<div id="vbid-59a5b-3lyrs2zz-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-ab048c8e-jlzblcqt-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div  id="vbid-59a5b-3lyrs2zz" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/eWaGqkBdwsI8pTfu-rzx3x3--TOAN6NYqmRcGlI9_shvW82ZIctRd_ZA0f0wNG1JHFPzJZsI9CpGY44PNEQ=s300);"  data-orig-width="600" data-orig-height="504" >
+
<div  id="vbid-ab048c8e-jlzblcqt" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/eWaGqkBdwsI8pTfu-rzx3x3--TOAN6NYqmRcGlI9_shvW82ZIctRd_ZA0f0wNG1JHFPzJZsI9CpGY44PNEQ=s300);"  data-orig-width="600" data-orig-height="504" >
 
<!-- VIDEO TEMPLATE -->
 
<!-- VIDEO TEMPLATE -->
 
 
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<div class="preview-title-holder removable-parent order-handle">
 
<div class="preview-title-holder removable-parent order-handle">
 
 
−
<h1 id="vbid-59a5b-7dnrzucs" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >EXPERIMENTS.<br></h1>
+
<h1 id="vbid-ab048c8e-ytu7qzah" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >EXPERIMENTS.<br></h1>
 
 
 
</div>
 
</div>
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<div class="preview-subtitle-holder removable-parent order-handle">
 
<div class="preview-subtitle-holder removable-parent order-handle">
 
 
−
<h3 id="vbid-59a5b-hdu3ogry" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">RESEARCH. PROTOCOLS. </h3>
+
<h3 id="vbid-ab048c8e-prbq3nwb" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">RESEARCH. PROTOCOLS. </h3>
  
 
</div>
 
</div>
Line 480: Line 432:
 
 
 
<!-- TEXT_BLOCK START -->
 
<!-- TEXT_BLOCK START -->
−
<div  id="vbid-59a5b-hi3nheu3"  class="master item-box  gallery-box style-59a5b-wansbqot         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-wansbqot" data-preview-styleid='style-59a5b-wansbqot' data-preset-type-id="TEXT_BLOCK">
+
<div  id="vbid-ab048c8e-ubacqqof"  class="master item-box  gallery-box style-ab048c8e-bqpytpac         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-ab048c8e-bqpytpac" data-preview-styleid='style-ab048c8e-bqpytpac' data-preset-type-id="TEXT_BLOCK">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 
 
−
<!-- CACHED VERSION (7) OF vbid-59a5b-hi3nheu3 FROM  22-06-2017 21:13:36 --><div  class="sub container style-59a5b-wansbqot content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-wansbqot" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-hi3nheu3" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-59a5b-wansbqot" data-style="style-4e411-j0xhjgzynh" data-absolute-path="http://edit.www.imcreator.com/viewer" >
+
<!-- CACHED VERSION (7) OF vbid-ab048c8e-ubacqqof FROM  31-07-2017 23:27:11 --><div  class="sub container style-ab048c8e-bqpytpac content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-ab048c8e-bqpytpac" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-ab048c8e-ubacqqof" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-ab048c8e-bqpytpac" data-style="style-4e411-j0xhjgzynh" data-absolute-path="https://xprs.imcreator.com/viewer" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
Line 509: Line 461:
 
 
 
 
−
<div  id="vbid-59a5b-39sl0kpx"  class="sub item-box  page-box style-59a5b-wansbqot         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-wansbqot" data-preview-styleid='style-59a5b-wansbqot' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-ab048c8e-m6fehd0f"  class="sub item-box  page-box style-ab048c8e-bqpytpac         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ab048c8e-bqpytpac" data-preview-styleid='style-ab048c8e-bqpytpac' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-39sl0kpx" data-preview-style="style-59a5b-wansbqot" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2304" data-orig-thumb-width="3456" data-vbid="vbid-59a5b-39sl0kpx" data-bgimg="https://lh3.googleusercontent.com/sjP4PlQklG7RdokajQMJUd3h4wz7G4uD4J0vFRBI9FEkbM05VwnaSprBtTcf02rloED8Qlc7_FkRHCj6cfM">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-ab048c8e-m6fehd0f" data-preview-style="style-ab048c8e-bqpytpac" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2304" data-orig-thumb-width="3456" data-vbid="vbid-ab048c8e-m6fehd0f" data-bgimg="https://lh3.googleusercontent.com/sjP4PlQklG7RdokajQMJUd3h4wz7G4uD4J0vFRBI9FEkbM05VwnaSprBtTcf02rloED8Qlc7_FkRHCj6cfM">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
<div class="Picture item-preview">
 
<div class="Picture item-preview">
Line 523: Line 475:
 
<div class="pic-side">
 
<div class="pic-side">
 
<div class="vertical-aligner">
 
<div class="vertical-aligner">
−
<div id="element-bf4b24176a4be6a-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-ab048c8e-lomkijeh-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div  id="element-bf4b24176a4be6a" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/sjP4PlQklG7RdokajQMJUd3h4wz7G4uD4J0vFRBI9FEkbM05VwnaSprBtTcf02rloED8Qlc7_FkRHCj6cfM=s300);"  data-orig-width="3456" data-orig-height="2304" >
+
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<div class="preview-subtitle-holder removable-parent order-handle">
 
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<h3 id="vbid-59a5b-edeab9t7" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">TICKETED CELL FREE EXPRESSION SYSTEM PROTOCOLS</h3>
+
<h3 id="vbid-ab048c8e-vgylddny" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">TICKETED CELL FREE EXPRESSION SYSTEM PROTOCOLS</h3>
  
 
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<div class="preview-body-holder removable-parent order-handle">
 
 
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<div id="vbid-59a5b-wwqdgekp" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
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<div id="vbid-ab048c8e-yn1mcpio" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 
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<!-- TEXT_BLOCK START -->
 
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<div  id="vbid-59a5b-qriiymks"  class="master item-box  gallery-box style-59a5b-c7ilk5ai         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-c7ilk5ai" data-preview-styleid='style-59a5b-c7ilk5ai' data-preset-type-id="TEXT_BLOCK">
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<div  id="vbid-ab048c8e-jus3pmve"  class="master item-box  gallery-box style-ab048c8e-4xnhoqcf         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-ab048c8e-4xnhoqcf" data-preview-styleid='style-ab048c8e-4xnhoqcf' data-preset-type-id="TEXT_BLOCK">
 
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<div id="no-image" class="stripe-background load-high-res " ></div>
 
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<!-- CACHED VERSION (7) OF vbid-ab048c8e-jus3pmve FROM  31-07-2017 23:27:11 --><div  class="sub container style-ab048c8e-4xnhoqcf content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-ab048c8e-4xnhoqcf" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-ab048c8e-jus3pmve" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-ab048c8e-4xnhoqcf" data-style="style-89a8e-bbtqrmxrkr" data-absolute-path="https://xprs.imcreator.com/viewer" >
 
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<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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<div  id="vbid-59a5b-nhphhuoi"  class="sub item-box  page-box style-59a5b-c7ilk5ai         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-c7ilk5ai" data-preview-styleid='style-59a5b-c7ilk5ai' data-preset-type-id="UNRESOLVED">
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<div  id="vbid-ab048c8e-sn8ru1kh"  class="sub item-box  page-box style-ab048c8e-4xnhoqcf         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ab048c8e-4xnhoqcf" data-preview-styleid='style-ab048c8e-4xnhoqcf' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-nhphhuoi" data-preview-style="style-59a5b-c7ilk5ai" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="480" data-orig-thumb-width="852" data-vbid="vbid-59a5b-nhphhuoi" data-bgimg="https://lh3.googleusercontent.com/JKUB_oN-40yfc7SvxBU4HecyxCCNDTIXgwyTByCTf1j5zCp9mg5nN8yqOO1FXPq2tppMG6hcXLcPu7FW">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-ab048c8e-sn8ru1kh" data-preview-style="style-ab048c8e-4xnhoqcf" data-style="style-89a8e-q4q4gr5nhr" data-orig-thumb-height="480" data-orig-thumb-width="852" data-vbid="vbid-ab048c8e-sn8ru1kh" data-bgimg="https://lh3.googleusercontent.com/JKUB_oN-40yfc7SvxBU4HecyxCCNDTIXgwyTByCTf1j5zCp9mg5nN8yqOO1FXPq2tppMG6hcXLcPu7FW">
 
<div  class="multi-container preview image-cover" >
 
<div  class="multi-container preview image-cover" >
 
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<div class="pic-side">
 
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<div id="element-9b0b621d711ada6-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-ab048c8e-hmgbdc6c-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
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<div  id="element-9b0b621d711ada6" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/JKUB_oN-40yfc7SvxBU4HecyxCCNDTIXgwyTByCTf1j5zCp9mg5nN8yqOO1FXPq2tppMG6hcXLcPu7FW=s300);"  data-orig-width="852" data-orig-height="480" >
+
<div  id="vbid-ab048c8e-hmgbdc6c" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/JKUB_oN-40yfc7SvxBU4HecyxCCNDTIXgwyTByCTf1j5zCp9mg5nN8yqOO1FXPq2tppMG6hcXLcPu7FW=s300);"  data-orig-width="852" data-orig-height="480" >
 
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<div class="preview-subtitle-holder removable-parent order-handle">
 
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−
<h3 id="vbid-59a5b-tzwvyglp" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">aPCR- Quantitative Polymerase Chain Reaction Protocol<br></h3>
+
<h3 id="vbid-ab048c8e-49s8yflj" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">qPCR- Quantitative Polymerase Chain Reaction Protocol<br></h3>
  
 
</div>
 
</div>
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<br class="upper-line-break" />
 
<br class="upper-line-break" />
 
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<div id="vbid-59a5b-vcstazwr" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
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<div id="vbid-ab048c8e-x3itnxx8" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 
</div>
 
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−
<div id="vbid-59a5b-ywifihmq" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+
<div id="vbid-ab048c8e-kazrniiz" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 
<p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 
<p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 
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<!-- TEXT_BLOCK START -->
 
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<div  id="vbid-59a5b-57oaabvj"  class="master item-box  gallery-box style-59a5b-bwc1xirn   button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-bwc1xirn" data-preview-styleid='style-59a5b-bwc1xirn' data-preset-type-id="TEXT_BLOCK">
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<div  id="vbid-ab048c8e-7yhdyxbb"  class="master item-box  gallery-box style-ab048c8e-dcan6uj9   button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-ab048c8e-dcan6uj9" data-preview-styleid='style-ab048c8e-dcan6uj9' data-preset-type-id="TEXT_BLOCK">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 
 
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<!-- CACHED VERSION (7) OF vbid-ab048c8e-7yhdyxbb FROM  31-07-2017 23:27:12 --><div  class="sub container style-ab048c8e-dcan6uj9 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-ab048c8e-dcan6uj9" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-ab048c8e-7yhdyxbb" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-ab048c8e-dcan6uj9" data-style="style-4e411-j0xhjgzynh" data-absolute-path="https://xprs.imcreator.com/viewer" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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<div  id="vbid-59a5b-3laacyxo"  class="sub item-box  page-box style-59a5b-bwc1xirn         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-bwc1xirn" data-preview-styleid='style-59a5b-bwc1xirn' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-ab048c8e-0njw7e29"  class="sub item-box  page-box style-ab048c8e-dcan6uj9         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ab048c8e-dcan6uj9" data-preview-styleid='style-ab048c8e-dcan6uj9' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-3laacyxo" data-preview-style="style-59a5b-bwc1xirn" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="4857" data-orig-thumb-width="1600" data-vbid="vbid-59a5b-3laacyxo" data-bgimg="https://lh3.googleusercontent.com/RA5j0HepG9cF2_DaVUMd8S32ljZZEzWqXazA4vFzyDl2Xg5M2IMl_sChycAlLSdh0uKPcWp974Yw-MM">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-ab048c8e-0njw7e29" data-preview-style="style-ab048c8e-dcan6uj9" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="4857" data-orig-thumb-width="1600" data-vbid="vbid-ab048c8e-0njw7e29" data-bgimg="https://lh3.googleusercontent.com/RA5j0HepG9cF2_DaVUMd8S32ljZZEzWqXazA4vFzyDl2Xg5M2IMl_sChycAlLSdh0uKPcWp974Yw-MM">
 
<div  class="multi-container preview image-cover" >
 
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<div id="vbid-59a5b-xb9fvk5m-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
+
<div id="vbid-ab048c8e-tkilrogy-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
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<div  id="vbid-59a5b-xb9fvk5m" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/RA5j0HepG9cF2_DaVUMd8S32ljZZEzWqXazA4vFzyDl2Xg5M2IMl_sChycAlLSdh0uKPcWp974Yw-MM=s300);"  data-orig-width="1600" data-orig-height="4857" >
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<div  id="vbid-ab048c8e-tkilrogy" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/RA5j0HepG9cF2_DaVUMd8S32ljZZEzWqXazA4vFzyDl2Xg5M2IMl_sChycAlLSdh0uKPcWp974Yw-MM=s300);"  data-orig-width="1600" data-orig-height="4857" >
 
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<div class="preview-title-holder removable-parent order-handle">
 
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−
<h2 id="vbid-59a5b-3aii51sg" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Experimental outline<br></h2>
+
<h2 id="vbid-ab048c8e-ewbdqz25" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Experimental outline<br></h2>
 
 
 
</div>
 
</div>
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<br class="upper-line-break" />
 
<br class="upper-line-break" />
 
<div class="preview-divider-holder removable-parent order-handle">
 
<div class="preview-divider-holder removable-parent order-handle">
−
<div id="vbid-59a5b-jnyxremf" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
+
<div id="vbid-ab048c8e-2hkgjjlp" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 
</div>
 
</div>
 
</div>
 
</div>
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<div class="preview-body-holder removable-parent order-handle">
 
<div class="preview-body-holder removable-parent order-handle">
 
 
−
<div id="vbid-59a5b-corz5lvg" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+
<div id="vbid-ab048c8e-avfb82og" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 
<p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 
<p class="MsoNormal" style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
 
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
 
margin-left:38.4pt;text-indent:-38.4pt;mso-text-indent-alt:-.25in;line-height:
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<div class="preview-social-wrapper removable-parent"  >
 
<div class="preview-social-wrapper removable-parent"  >
−
<div id="vbid-59a5b-xmkwjkjw" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="1" >
+
<div id="vbid-59a5b-xmkwjkjw" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="7" >
 
 
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<div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="//edit.www.imcreator.com/images/socialmedia/1facebook.png">
+
<div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="//xprs.imcreator.com/images/socialmedia/7facebook.png">
−
<a class='social-link-url' href="http://www.facebook.com/" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/1facebook.png" ></a>
+
<a class='social-link-url' href="https://www.facebook.com/RAINIncubator" target='_blank'><img class='preview-link-img ' src="//xprs.imcreator.com/images/socialmedia/7facebook.png" ></a>
 
</div>
 
</div>
 
 
−
<div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="//edit.www.imcreator.com/images/socialmedia/1twitter.png">
+
<div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="//xprs.imcreator.com/images/socialmedia/7twitter.png">
−
<a class='social-link-url' href="http://www.twitter.com/" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/1twitter.png" ></a>
+
<a class='social-link-url' href="https://twitter.com/RAINincubator" target='_blank'><img class='preview-link-img ' src="//xprs.imcreator.com/images/socialmedia/7twitter.png" ></a>
 
</div>
 
</div>
 
 
−
<div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="//edit.www.imcreator.com/images/socialmedia/1instagram.png">
+
<div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="//xprs.imcreator.com/images/socialmedia/7instagram.png">
−
<a class='social-link-url' href="http://www.instagram.com" target='_blank'><img class='preview-link-img ' src="//edit.www.imcreator.com/images/socialmedia/1instagram.png" ></a>
+
<a class='social-link-url' href="https://www.linkedin.com/in/rain-network" target='_blank'><img class='preview-link-img ' src="//xprs.imcreator.com/images/socialmedia/7instagram.png" ></a>
 
</div>
 
</div>
 
 
−
<div id="YOUTUBE" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="YOUTUBE" data-img-url="//edit.www.imcreator.com/images/socialmedia/1youtube.png">
+
<div id="YOUTUBE" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="YOUTUBE" data-img-url="//xprs.imcreator.com/images/socialmedia/7youtube.png">
−
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Revision as of 23:28, 31 July 2017

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Experiments - Home


EXPERIMENTS.



RESEARCH. PROTOCOLS.



TICKETED CELL FREE EXPRESSION SYSTEM PROTOCOLS



I. Overview

The idea of cell free expression has existed for more than 20 years. It is the notion of taking the elements required for both transcription and translation and utilizing them for an external protein expression system. This is extremely beneficial for expression of cytotoxic proteins in which high yields are difficult to produce in vivo. For our purpose within this assay, this concept is utilized in conjunction with a regulatory factor for detection. This is detection is predicated either by regulation of the RBS at the transcript level or by sequestering the binding sight of RNA polymerase.

II. Work Flow

§ Identify trigger sequence and target plasmid or probe

§ Add target

§ Incubation

§ Evaluation

III. Reagents

§ Cell free expression kit

§ •Promega L5540

§ •Promega L1020

§ •Promega L1110

§ •NEB E6800L

§ •Life Tech K990100

§ RNASE Inhibitor (RI)

§ Prepared tickets

§ Trigger Plasmid

§ Substrate if needed



qPCR- Quantitative Polymerase Chain Reaction Protocol





I. Overview

The idea of cell free expression has existed for more than 20 years. It is the notion of taking the elements required for both transcription and translation and utilizing them for an external protein expression system. This is extremely beneficial for expression of cytotoxic proteins in which high yields are difficult to produce in vivo. For our purpose within this assay, this concept is utilized in conjunction with a regulatory factor for detection. This is detection is predicated either by regulation of the RBS at the transcript level or by sequestering the binding sight of RNA polymerase.

II. Work Flow

§ Identify trigger sequence and target plasmid or probe

§ Add target

§ Incubation

§ Evaluation

III. Reagents

§ Cell free expression kit

§ •Promega L5540

§ •Promega L1020

§ •Promega L1110

§ •NEB E6800L

§ •Life Tech K990100

§ RNASE Inhibitor (RI)

§ Prepared tickets

§ Trigger Plasmid

§ Substrate if needed



Experimental outline





I. Pre-Phase One Experiments

Establish control experiments

§ -Constitutive GFP

§ o Switch H GFP

II. Pre-Phase One Goals

§ The goal of pre-phase 1 is to establish a working positive control and negative control in lab.

III. Phase One Experiments

§ Clone composite circuit in e.coli

§ Test efficacy of the circuit in vivo and determine threshold limits of both arsenic and arsenite

§ Perform mini prep and quantify

IV. Phase One Goals

§ The goals of this phase is to grow a large amount of the composite circuit and develop an understanding of whether or not the circuit is biologically effective. Another primary goal is to develop an understanding of how the circuit works in its entirety (upper threshold/lower threshold)

V. Phase Two Experiments

§ Test the efficacy of the composite circuit instead of crude lysate

§ Test purified plasmid with Promega S30 kit

VI. Phase Two Goals

§ The goal of this phase is to take the circuit out of the cell and test it in situ utilizing both a bacterial expression kit (Promega S30) and an attempt at functionalizing the circuit utilizing a formulated crude lysate for increased application space.

VII. Phase Three Experiments

§ Attempt at micro scaling reaction

§ Porting reactions to paper based system

§ Attempt at lyophilizing the circuit on paper both with Promega S30 kit and in the crude lysate form (if the previous phase demonstrated reasonable evidence for progressing on that front)

§ Phase Three Goals

§ The goal of this phase is to successfully micro scale the reaction and port it to a lateral flow paper based system. If successful in this phase, we will have developed a biological means to detect presence of arsenic and arsenite utilizing a lateral flow paper system that is both cost effective and easy to implement.


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