Difference between revisions of "Team:BOKU-Vienna/Protocol"
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<p>The Interlab Challenge was performed according to the protocols for the InterLab Study in 2017. For further details visit the iGEM homepage: <a href="https://2017.igem.org/Competition/InterLab_Study" target="_blank">InterLab Study</a> or our <a href=" https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">InterLab page</a>.</p> | <p>The Interlab Challenge was performed according to the protocols for the InterLab Study in 2017. For further details visit the iGEM homepage: <a href="https://2017.igem.org/Competition/InterLab_Study" target="_blank">InterLab Study</a> or our <a href=" https://2017.igem.org/Team:BOKU-Vienna/InterLab" target="_blank">InterLab page</a>.</p> | ||
<h5>Kit Plate 6 InterLab Part Locations</h5> | <h5>Kit Plate 6 InterLab Part Locations</h5> | ||
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Revision as of 15:30, 26 July 2017
Protocol
Protocols from A-Z.
Our protocols will follow shortly.
Contents
Materials
LB medium - E. Coli
| Name | Amount |
|---|---|
| Hy Soy Peptone | 10.0g |
| Yeast Extract | 5.0g |
| NaCl | 5.0g |
| RO-water | 1000 mL |
LB Agar - E. Coli
| Name | Amount |
|---|---|
| Hy soy Peptone | 10.0g |
| Yeast Extract | 5.0g |
| NaCl | 5.0g |
| RO-water | 980 mL |
| Agar-Agar per 500mL | 7g |
Methods
Transformation of chemically competent E. Coli
Competent cells from E. Coli strain DH5α or DH10B were thawed on ice. The whole ligation/other DNA samples were added to the cells, for 30 min the mix was put on ice. The cells and DNA mix were heat shocked for 90s at 42°C. The cells recovered on ice for 5min. 1 mL of LB medium was added to the cells and then an incubation followed at 37°C in a shaker-incubator for 45min. An appropiate amount of cells were plated on a selective LB agar.
Miniprep
Minipreps were performed using Hi Yield®Plasmid Mini DNA Isolationkit according to the manufacturer's protocols.
InterLab Study
The Interlab Challenge was performed according to the protocols for the InterLab Study in 2017. For further details visit the iGEM homepage: InterLab Study or our InterLab page.
Kit Plate 6 InterLab Part Locations
| Name | Backbone |
|---|---|
| Positive Control | BBa_I20270 |
| Negative Control | BBa_R0040 |
| Test Device 1 | BBa_J364000 |
| Test Device 2 | BBa_J364001 |
| Test Device 3 | BBa_J364002 |
| Test Device 4 | BBa_J364003 |
| Test Device 5 | BBa_J364004 |
| Test Device 6 | BBa_J364005 |
