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+ | |||
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+ | |||
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+ | |||
+ | <span id="643d5085" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span> | ||
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</a> | </a> | ||
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<div class="gallery-wrapper item-wrapper promo-wrapper "> | <div class="gallery-wrapper item-wrapper promo-wrapper "> | ||
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></div> | ></div> | ||
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+ | <h1 id="vbid-e71ead39-snunw6rd" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Our circuit was designed to utilize specific parts so that when the E. coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.</h1> | ||
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− | <h2 id="vbid- | + | <h2 id="vbid-337bd424-4wahkg6v" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Demonstrate the Circuit<br><br>Before running the actual circuit, we ran control experiments to determine whether or not the GFP would indeed function as a reporter, meaning it is expressed by the plasmid.<br><br>The first control experiment drove GFP using a constitutive promoter: meaning that the GFP would always be produced. This was essentially only used to ensure that the GFP works in the paper ticket.<br><br>The second control experiment was regulated, meaning that GFP expression was dependent on a trigger. This tested if the GFP could be controlled/ regulated.<br><br>These experiments were performed using a commercial in vitro transcription/translation kit by Promega.<br><br>The results from both of these experiments are shown in the pictures below.<br></h2> |
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+ | <!-- ARRANGER SETTINGS --> | ||
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+ | ></div> | ||
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+ | <div class="page-wrapper item-wrapper "> | ||
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+ | <div class="helper-div middle" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="text-side "> | ||
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+ | <h3 id="vbid-7ce2a162-91qcmaki" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">This ticket is representative of the first experiment where we expressed GFP constitutively in the first column. The second column contains water, which was the negative control.</h3> | ||
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− | <div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="// | + | <div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="https://static.igem.org/mediawiki/2017/a/a4/FacebookImage.png"> |
− | <a class='social-link-url' href=" | + | <a class='social-link-url' href="https://www.facebook.com/RAINIncubator" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/a/a4/FacebookImage.png" ></a> |
</div> | </div> | ||
− | <div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="// | + | <div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="https://static.igem.org/mediawiki/2017/5/59/TwitterImage.png"> |
− | <a class='social-link-url' href=" | + | <a class='social-link-url' href="https://twitter.com/RAINincubator" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/5/59/TwitterImage.png" ></a> |
</div> | </div> | ||
− | <div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="// | + | <div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="https://static.igem.org/mediawiki/2017/8/86/InstagramImage.png"> |
− | <a class='social-link-url' href=" | + | <a class='social-link-url' href="https://www.linkedin.com/in/rain-network" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/8/86/InstagramImage.png" ></a> |
</div> | </div> | ||
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− | <a class='social-link-url' href=" | + | <a class='social-link-url' href="https://www.youtube.com/channel/UCuVc4cKxuUzpjZfywBgoT9w" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/3/34/YoutubeImage.png" ></a> |
</div> | </div> | ||
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− | <a class='social-link-url' href=" | + | <a class='social-link-url' href="https://plus.google.com/u/0/+RainincubatorOrg" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/f/fa/GooglePlusImage.png" ></a> |
</div> | </div> | ||
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− | <script src="// | + | <script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lightbox?action=raw&ctype=text/javascript"></script> |
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Revision as of 16:42, 28 October 2017