|
|
| Line 350: |
Line 350: |
| | </div> | | </div> |
| | <div class="menu-container"> | | <div class="menu-container"> |
| − | <div style="position: absolute; left: 13px; top: 10px; height: 110px; width: 2px; background: #0096ff; z-index: -1;"></div> | + | <div style="position: absolute; left: 13px; top: 10px; height: 70px; width: 2px; background: #0096ff; z-index: -1;"></div> |
| − | <div class="item active"> | + | <div class="item active" data-key="1"> |
| | + | <div class="marker"><div></div></div> |
| | + | <div class="text">Intro</div> |
| | + | </div> |
| | + | <div class="item" data-key="2"> |
| | <div class="marker"><div></div></div> | | <div class="marker"><div></div></div> |
| | <div class="text">The problem</div> | | <div class="text">The problem</div> |
| | </div> | | </div> |
| − | <div class="item"> | + | <div class="item" data-key="3"> |
| | <div class="marker"><div></div></div> | | <div class="marker"><div></div></div> |
| | <div class="text">The system</div> | | <div class="text">The system</div> |
| − | </div>
| |
| − | <div class="item">
| |
| − | <div class="marker"><div></div></div>
| |
| − | <div class="text">Subheading 3</div>
| |
| − | </div>
| |
| − | <div class="item">
| |
| − | <div class="marker"><div></div></div>
| |
| − | <div class="text">Subheading 4</div>
| |
| | </div> | | </div> |
| | </div> | | </div> |
| Line 376: |
Line 372: |
| | <br /> | | <br /> |
| | <br /> | | <br /> |
| − | <h2>The problem</h2> | + | <h2 class="subhead" id="subhead-2">The problem</h2> |
| | Disease diagnosis is of great importance for healthcare. In developing countries, diagnoses often have to be made based on limited information, even though accurate disease determination based on pathogen specific DNA sequences is possible through sequencing technologies. These technologies, however, require specialised equipment and expertise that simply is not available everywhere. The OUTCASST two-component system and detection kit hopes to alleviate this problem. | | Disease diagnosis is of great importance for healthcare. In developing countries, diagnoses often have to be made based on limited information, even though accurate disease determination based on pathogen specific DNA sequences is possible through sequencing technologies. These technologies, however, require specialised equipment and expertise that simply is not available everywhere. The OUTCASST two-component system and detection kit hopes to alleviate this problem. |
| | | | |
| Line 404: |
Line 400: |
| | </center> | | </center> |
| | | | |
| − | <h2>The system</h2> | + | <h2 class="subhead" id="subhead-3">The system</h2> |
| | The OUTCASST two-component system consists of two proteins, expressed to the membrane of a dryable cell. One of the proteins is a Cas9-fusion and the other contains Cpf1. Both proteins can be given a guide RNA that makes it bind to a specific, user-chosen, complementary sequence. When both proteins bind a DNA fragment from a sample, they co-localize, so that a transcription factor is released intracellularly which then induces an intracellular reporter mechanism such as a dye or fluorescent signal. | | The OUTCASST two-component system consists of two proteins, expressed to the membrane of a dryable cell. One of the proteins is a Cas9-fusion and the other contains Cpf1. Both proteins can be given a guide RNA that makes it bind to a specific, user-chosen, complementary sequence. When both proteins bind a DNA fragment from a sample, they co-localize, so that a transcription factor is released intracellularly which then induces an intracellular reporter mechanism such as a dye or fluorescent signal. |
| | | | |
| Line 473: |
Line 469: |
| | <script type="text/javascript"> | | <script type="text/javascript"> |
| | $.noConflict(); | | $.noConflict(); |
| | + | |
| | + | function getPos(el) |
| | + | { |
| | + | for (var lx=0, ly=0; |
| | + | el != null; |
| | + | lx += el.offsetLeft, ly += el.offsetTop, el = el.offsetParent); |
| | + | return {x: lx,y: ly}; |
| | + | } |
| | + | |
| | + | jQuery( window ).scroll(function() { |
| | + | var sel_subhead = 1; |
| | + | |
| | + | jQuery(".subhead").each(function(k, v) |
| | + | { |
| | + | var key = v.id.split("-")[1]; |
| | + | |
| | + | var pos = getPos(v); |
| | + | |
| | + | if(document.body.scrollTop + 200 >= pos.y) |
| | + | { |
| | + | sel_subhead = key; |
| | + | } |
| | + | else if(key == 3 && document.body.scrollTop + document.body.getHeight() == document.body.scrollHeight) |
| | + | { |
| | + | sel_subhead = key; |
| | + | } |
| | + | }); |
| | + | |
| | + | jQuery(".menu-container .item").each(function(k, v) |
| | + | { |
| | + | var key = v.getAttribute("data-key"); |
| | + | |
| | + | if(key == sel_subhead) |
| | + | jQuery(v).addClass("active"); |
| | + | else |
| | + | jQuery(v).removeClass("active"); |
| | + | }); |
| | + | }); |
| | | | |
| | jQuery(function(){ | | jQuery(function(){ |
The OUTCASST two-component system
This year, Utrecht University participates in the iGEM for the first time. We aim to create a cheap DNA detection kit for disease diagnosis that is easy to use and does not rely on complicated sequencing technologies.
The problem
Disease diagnosis is of great importance for healthcare. In developing countries, diagnoses often have to be made based on limited information, even though accurate disease determination based on pathogen specific DNA sequences is possible through sequencing technologies. These technologies, however, require specialised equipment and expertise that simply is not available everywhere. The OUTCASST two-component system and detection kit hopes to alleviate this problem.
The system
The OUTCASST two-component system consists of two proteins, expressed to the membrane of a dryable cell. One of the proteins is a Cas9-fusion and the other contains Cpf1. Both proteins can be given a guide RNA that makes it bind to a specific, user-chosen, complementary sequence. When both proteins bind a DNA fragment from a sample, they co-localize, so that a transcription factor is released intracellularly which then induces an intracellular reporter mechanism such as a dye or fluorescent signal.
Binding of components with search-specific gRNA sequences.
Next
DNA sample fragment binds to one of the components.
Next
Fragment binding with both components induces co-localization.
Next
Protease cleaves, transcription factor is released from complex.
