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<h3>Experiment</h3> | <h3>Experiment</h3> | ||
iGEM provided 8 plasmids for the InterLab Study. Devices 1-6 and positive control have the same reporter gene (GFP), terminator (B0015) and backbone (pSB1C3). However, devices 1-3 share the same RBS (B0034), while devices 4-6 share another modified RBS called bicistronic device (BCD2). Different promoters are also used in different plasmid. According to the strength of promoter described by iGEM2006_Berkeley team, device 1 should have the strongest fluorescence and device 3 should have the weakest among devices 1-3, while Device 4 should have the strongest fluorescence and device 6 should have the weakest among devices 4-6. | iGEM provided 8 plasmids for the InterLab Study. Devices 1-6 and positive control have the same reporter gene (GFP), terminator (B0015) and backbone (pSB1C3). However, devices 1-3 share the same RBS (B0034), while devices 4-6 share another modified RBS called bicistronic device (BCD2). Different promoters are also used in different plasmid. According to the strength of promoter described by iGEM2006_Berkeley team, device 1 should have the strongest fluorescence and device 3 should have the weakest among devices 1-3, while Device 4 should have the strongest fluorescence and device 6 should have the weakest among devices 4-6. | ||
+ | |||
+ | <table width="80%"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="6"><u>Summary of parts measured</u></th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Promoter (strength)</th> | ||
+ | <th>RBS</th> | ||
+ | <th>Coding Sequence</th> | ||
+ | <th>Terminator</th> | ||
+ | <th>Backbone</th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Device 1 (J364000)</td> | ||
+ | <td>J23101(1791)</td> | ||
+ | <td rowspan="2">B0034</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </table> | ||
+ | |||
+ | |||
<h3>What is Bicistronic Device (BCD)?</h3> | <h3>What is Bicistronic Device (BCD)?</h3> |
Revision as of 18:02, 7 September 2017