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<td> <top> <img class="media object pull-left" src="https://static.igem.org/mediawiki/2017/8/81/T--Macquarie_Australia--flasklogo_.png" alt="flasklogo" style= "height:50%; width:50%"> | <td> <top> <img class="media object pull-left" src="https://static.igem.org/mediawiki/2017/8/81/T--Macquarie_Australia--flasklogo_.png" alt="flasklogo" style= "height:50%; width:50%"> | ||
</td> | </td> | ||
+ | <td> | ||
<h4> Wet Lab </h4> | <h4> Wet Lab </h4> | ||
<ul> | <ul> | ||
− | <li> </li> | + | <li> Sequencing results came back and unfortunately our Fer/Hyd plasmid was lacking Hyd 1. </li> |
+ | <ul> <li> This was unexpected. </li> </ul> | ||
+ | <li> We miniprep extracted Fer/Hyd plasmid from the three new colonies which grew from Rob’s cells, as well as the one colony we achieved from previous cells spun down. </li> | ||
+ | <ul> <li> Once run on gel, two from Rob’s cells (A and B) looked promising so these have been sent off for sequencing.</li> </ul> | ||
+ | <li> A new ligation was made with HydEFG and Thi’s batch of cells transformed with this new construct. </li> | ||
+ | <ul> <li> Unfortunately, nothing grew. </li> | ||
+ | <li> With 5ul of HydEFG plasmid still available we decided we should reduce our transformations to use only 2ul. </li> | ||
+ | <li> This would leave us enough to digest and run on a gel next week. </li> | ||
+ | <li> The plan was to make up new competent cells as it appeared the cells we are using were not as competent as expected. </li> | ||
+ | <li> Keen to keep going we proceeded with commercially available competent cells of our next HydEFG transformation attempt.</li> </ul> | ||
+ | <li>Running a gel showed that the latest attempt to PCR amplify the three backbones- Amp, CAM, Kan, was not successful. </li> | ||
+ | <ul> <li> Another attempt has been made to achieve this using different templates.</li> | ||
+ | <li> We plan to run these on a gel next week. </li> </ul> | ||
</ul> | </ul> | ||
+ | </td> | ||
</tr> | </tr> | ||
</table> | </table> |
Revision as of 14:19, 10 September 2017
Notebook
Glossary
Week 1 (3/7/17-7/7/17)
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Dry Lab
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Week 2 (10/7/17-14/7/17)
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Dry Lab
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Wet Lab
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Week 3 (17/7/17-21/7/17)
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Dry Lab
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Wet Lab
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Week 4 (24/7/17-28/7/17)
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Dry Lab
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Wet Lab
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Week 5 (31/7/17-4/7/17)
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Dry Lab
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Wet Lab
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Week 6 (7/8/17-11/8/17)
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Dry Lab
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Wet LabOur plan was as follows:
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Week 7 (14/8/17-18/8/17)
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Dry Lab |
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Wet Lab
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Week 8 (21/8/17-25/8/17)
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Dry Lab |
Wet Lab |
Week 9 (28/8/17-1/9/17)
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Dry Lab |
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Wet Lab |
Week 10 (4/9/17-8/9/17)
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Dry Lab |
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Wet Lab |
Week 11 (22/9/17-15/9/17)
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Dry Lab |
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Wet Lab |
Week 12 (18/9/17-22/9/17)
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Dry Lab |
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Wet Lab |
Week 13 (25/9/17-29/9/17)
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Dry Lab |
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Wet Lab |
Week 14 (2/10/17-6/10/17)
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Dry Lab |
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Wet Lab
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Week 15 (9/10/17-13/10/17)
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Dry Lab> |
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Wet Lab |
Week 16 (16/10/17-20/10/17)
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Dry Lab |
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Wet Lab |
Week 17 (23/10/17-27/10/17)
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Dry Lab |
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Wet Lab |
Week 18 (30/10/17-3/10/17)
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Dry Lab |
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Wet Lab |