Difference between revisions of "Team:Hong Kong-CUHK/Results"

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Revision as of 16:41, 14 September 2017




Results

Toehold Switch

This is overview about Toehold Switch.

Interlab

Please refer to the Interlab part.

Characterization

Overview
Fluorescent protein is an important marker for qualitative analysis in validation test of Toehold switch which detects the presence of trigger (viral) RNA.The presence of trigger RNA is reported by fold change of fluorescent protein expression compared with switch only by fluorescent assays and rely on the ability of fluorescent protein expression. More often, body fluid of different pH from vary organs extracted as sample may disturb the fluorescent level due to pH-dependent folding and conformation of proteins.
To determine the effective range and optimize the protein expression level for clinical diagnosis, we have demonstrated their pH stability under different pH condition.

Key Achievements
Characterised two fluorescent proteins for the registry to functionalize Toehold switch for clinical diagnosis. Proved the pH dependent stability of fluorescent proteins.

Program

Program

     

Email: igemcuhk1617@gmail.com