Difference between revisions of "Team:Cadets2Vets/Description"

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<span id="b951bd16"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
 
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<span id="928ba103"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 
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<span id="e08666cf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook-July</span>
 
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<span id="bd841f1b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 
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<span id="a6cf4805"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
 
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<span id="element-077b707f6118c36"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
 
<span id="element-077b707f6118c36"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
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<span id="2ed4772d"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook-July</span>
 
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<span id="ece571a0"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 
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<span id="463d44e3"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 
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<span id="ae4d1fb8"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
 
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<span id="df3fda7a"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook-June</span>
 
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<span id="67d43db3"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Why Arsenic</span>
 
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<p>The goal of this project is to engineer a biological circuit utilizing multiple e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic. Once this construct is designed and constructed the circuit will be tested to demonstrate is viability using a cell free lateral flow paper based system deigned to detect Arsenic.</p>
+
<p>The goal of this project is to engineer a biological circuit utilizing multiple <em>E.coli</em> DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect arsenic. Once this construct is designed and created, the circuit will be tested to demonstrate its viability using a cell-free lateral flow paper-based system. The circuit is designed to detect arsenic in environmental samples.</p>
 
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<p><span id="docs-internal-guid-3af18dd3-9a26-83ac-e2be-c4e4a1066252"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">The ASARCO smelting company smokestack, erected in 1917, was at the time a 571-foot tall symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area, but with it, a price we are still paying for today. Over the course of the almost century the plant was operational, it had been pumping, along with other toxic materials, heavy metals like arsenic and lead directly into the air where it then settled into the surrounding soil and water of the greater Tacoma area. The smokestack was demolished in 1985, but over 30 years later there is still a toxic level of heavy metals in the soil. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.</span></span></p>
 
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<p><span id="docs-internal-guid-3af18dd3-9a28-a43b-0ece-d8b8a72d568d"><span style="font-size: 12pt; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">In 2007, 32 of 191 parks examined by the Department of Ecology showed, and it was determined that the parks required action due to toxic levels of arsenic and lead. Vassault Park in Tacoma is an example of just one of these parks. Located just a few miles from the former ASARCO plant, the soil of the park’s large baseball and soccer fields was found to contain roughly 30 times the natural arsenic soil concentration. As such, the city of Tacoma began the 2.5 million dollar project of resurfacing the park in 2014. The project was completed in 2015. Similar expensive projects have been completed in the parks of the greater Tacoma area, including further testing at one of the area's largest parks: Point Defiance. Despite this urban upheaval, there are still unsafe levels of toxic materials in the homes and neighborhoods of Tacoma today. Cleaning our city of heavy metals is a project that has been in the works for the past 30 years. Awareness and safety measures continue to expand, but it is most likely one that the problem will continue to affect residents for years.</span></span></p>
 
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<p>Throughout this project we worked closely with several local non-profits in the Tacoma urban area. The goal of the project was to design a successful biological circuit utilizing e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic+.</p>
+
<p>Throughout this project, we worked closely with several local non-profits in the Tacoma urban area. The goal of the project was to design a successful biological circuit utilizing <em>E.coli</em> DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect arsenic.</p>
 
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Revision as of 17:16, 16 September 2017

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Description - Home


OUR PROJECT



The goal of this project is to engineer a biological circuit utilizing multiple E.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect arsenic. Once this construct is designed and created, the circuit will be tested to demonstrate its viability using a cell-free lateral flow paper-based system. The circuit is designed to detect arsenic in environmental samples.



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Goal #1



Develop tools to work at increasing levels of abstraction and enable facile manufacturing of designs



Goal #2



Leverage expertise from established engineering disciplines



Goal #3



Ultimately, forward engineer novel solutions to diverse problems



RESOURCES



Throughout this project, we worked closely with several local non-profits in the Tacoma urban area. The goal of the project was to design a successful biological circuit utilizing E.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect arsenic.



After the circuit was designed and constructed the next step was to demonstrate the circuit as viable in a cell of Arsenic. To accomplish this goal we worked with Urban Waters, Readiness Acceleration and Innovation Networking (RAIN),and Charles Wright Academy.



By utilizing community involvement we hope to both increase pre-collegiate interest in STEM related fields as well as increase the communal understanding of the environment and provide a inexpensive and effective tool for the detection of potentially toxic metals.


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2304 Jefferson Ave, Tacoma, WA 98402