Difference between revisions of "Team:Utrecht"

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#REDIRECT [[Team:Utrecht/]]
 
#REDIRECT [[Team:Utrecht/]]
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<li data-key="hp">Human practices</li>
 
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<br />
 
<br />
 
 
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<div class="text">Cas9 & Cpf1 secretion<br/>and activity</div>
 
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<div class="text">Meet our team</div>
 
<div class="desc">About us, our interests and roles in the team and our supervisors.</div>
 
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<div class="section">
 
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<div class="text">Sponsors</div>
 
<div class="desc">A listing of our sponsors, how they assisted us and our gratitude for their assistance.</div>
 
</div>
 
<div class="section">
 
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<div class="text">Intro</div>
 
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<div class="text">The problem</div>
 
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<div class="page-heading">The OUTCASST two-component system</div>
 
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This year, Utrecht University participates in the iGEM for the first time. We aim to create a cheap DNA detection kit for disease diagnosis that is easy to use and does not rely on complicated sequencing technologies.
 
 
<br />
 
<br />
 
<h2 class="subhead" id="subhead-2">The problem</h2>
 
Disease diagnosis is of great importance for healthcare. In developing countries, diagnoses often have to be made based on limited information, even though accurate disease determination based on pathogen specific DNA sequences is possible through sequencing technologies. These technologies, however, require specialised equipment and expertise that simply is not available everywhere. The OUTCASST two-component system and detection kit hopes to alleviate this problem.
 
 
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<h2 class="subhead" id="subhead-3">The system</h2>
 
The OUTCASST two-component system consists of two proteins, expressed to the membrane of a dryable cell. One of the proteins is a Cas9-fusion and the other contains Cpf1. Both proteins can be given a guide RNA that makes it bind to a specific, user-chosen, complementary sequence. When both proteins bind a DNA fragment from a sample, they co-localize, so that a transcription factor is released intracellularly which then induces an intracellular reporter mechanism such as a dye or fluorescent signal.
 
 
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Binding of components with search-specific gRNA sequences.
 
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DNA sample fragment binds to one of the components.
 
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Fragment binding with both components induces co-localization.
 
<br>
 
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Protease cleaves, transcription factor is released from complex.
 
<br>
 
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Latest revision as of 17:43, 17 September 2017

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