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| | <div class="desc">Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.</div> | | <div class="desc">Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="outcasst"> |
| | <div class="thumb"></div> | | <div class="thumb"></div> |
| | <div class="text">OUTCASST system production</div> | | <div class="text">OUTCASST system production</div> |
| | <div class="desc">Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.</div> | | <div class="desc">Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="modeling-and-mathematics"> |
| | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/6/6b/Modelling.png"></div> | | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/6/6b/Modelling.png"></div> |
| | <div class="text">Modeling and<br />mathematics</div> | | <div class="text">Modeling and<br />mathematics</div> |
| | <div class="desc">Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.</div> | | <div class="desc">Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="interlab-study"> |
| | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/6/63/Interlab1.png"></div> | | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/6/63/Interlab1.png"></div> |
| | <div class="text">Interlab study participation</div> | | <div class="text">Interlab study participation</div> |
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| | <div class="sections" data-key="hp"> | | <div class="sections" data-key="hp"> |
| − | <div class="section"> | + | <div class="section" data-url="stakeholders"> |
| | <div class="thumb"></div> | | <div class="thumb"></div> |
| | <div class="text">Stakeholders & opinions</div> | | <div class="text">Stakeholders & opinions</div> |
| | <div class="desc">Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.</div> | | <div class="desc">Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="safety"> |
| | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/8/8d/Igemutrecht_safety.png"></div> | | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/8/8d/Igemutrecht_safety.png"></div> |
| | <div class="text">Risks & safety-issues</div> | | <div class="text">Risks & safety-issues</div> |
| | <div class="desc">Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.</div> | | <div class="desc">Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="product-design"> |
| | <div class="thumb"></div> | | <div class="thumb"></div> |
| | <div class="text">Design & integration</div> | | <div class="text">Design & integration</div> |
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| | <div class="sections" data-key="team"> | | <div class="sections" data-key="team"> |
| − | <div class="section"> | + | <div class="section" data-url="team"> |
| | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/5/5e/Team_comp.png"></div> | | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/5/5e/Team_comp.png"></div> |
| | <div class="text">Meet our team</div> | | <div class="text">Meet our team</div> |
| | <div class="desc">About us, our interests and roles in the team and our supervisors.</div> | | <div class="desc">About us, our interests and roles in the team and our supervisors.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="sponsors"> |
| | <div class="thumb"></div> | | <div class="thumb"></div> |
| | <div class="text">Sponsors</div> | | <div class="text">Sponsors</div> |
| | <div class="desc">A listing of our sponsors, how they assisted us and our gratitude for their assistance.</div> | | <div class="desc">A listing of our sponsors, how they assisted us and our gratitude for their assistance.</div> |
| | </div> | | </div> |
| − | <div class="section"> | + | <div class="section" data-url="achievements"> |
| | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/6/64/Achievements.png"></div> | | <div class="thumb"><img height="100" src="https://static.igem.org/mediawiki/2017/6/64/Achievements.png"></div> |
| | <div class="text">Achievements</div> | | <div class="text">Achievements</div> |
The OUTCASST two-component system
This year, Utrecht University participates in the iGEM for the first time. We aim to create a cheap DNA detection kit for disease diagnosis that is easy to use and does not rely on complicated sequencing technologies.
The problem
Disease diagnosis is of great importance for healthcare. In developing countries, diagnoses often have to be made based on limited information, even though accurate disease determination based on pathogen specific DNA sequences is possible through sequencing technologies. These technologies, however, require specialised equipment and expertise that simply is not available everywhere. The OUTCASST two-component system and detection kit hopes to alleviate this problem.
The system
The OUTCASST two-component system consists of two proteins, expressed to the membrane of a dryable cell. One of the proteins is a Cas9-fusion and the other contains Cpf1. Both proteins can be given a guide RNA that makes it bind to a specific, user-chosen, complementary sequence. When both proteins bind a DNA fragment from a sample, they co-localize, so that a transcription factor is released intracellularly which then induces an intracellular reporter mechanism such as a dye or fluorescent signal.
Binding of components with search-specific gRNA sequences.
Next
DNA sample fragment binds to one of the components.
Next
Fragment binding with both components induces co-localization.
Next
Protease cleaves, transcription factor is released from complex.
