Difference between revisions of "Team:INSA-UPS France/test/description"

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         <p>Press &amp; Media</p>
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         <p>Model overview</p>
 
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     <h1>Aims of the model</h1>
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         Our strategy was based on an input with a quorum sensing molecule production, a molecular communication between two organisms, and an output with the antimicrobial peptides effect. We needed to use a model to simulate this biological system and analyze if this communication was feasible:
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         Would the quorum sensing molecule (CAI-1) induce a sufficient answer to activate the sensor (<i>Vibrio harveyi</i>)? Would the receptor be able to produce enough molecular message (diacetyl) to communicate with the effector <i>Pichia pastoris</i>? Would the effector produce enough antimicrobial peptides to deliver the guessed output, which is the lysis of <i>V. cholerae</i> to reach a non-toxic concentration?
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         A model was also crucial regarding the entrepreneurship and the integrated human practices parts of our project: we needed to show to clients and investors, but also to citizens, how our system would work, how we will dimensionate our device, and how long do you have to wait before drinking a non-contaminated water.
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         To sum up, the goals of our model could be sum up into four objectives:
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         <li>Demonstrate the feasibility</li>
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        <li>Confirm our wet lab strategy</li>
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        <li>Dimensionate</li>
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        <li>Estimate the waiting time</li>
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       <h1>Approaches</h1>
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         Two complementary approaches have been used to represent our model. Working with a complex biological system involving three microorganisms and several molecules, the Systemic Biology Graphical Notation (SBGN) was a perfect way to ordinate the system and represent the interactions between organisms and the molecules involved.
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          The <i>20 minutes</i> is a daily free generalist newspaper aimed at commuters who want quick and concise information, reaching a wide audience. We appeared in the regional section of the online version of it. Thus we were pleased to reach the local inhabitants to mobilize them and make them know about science initiatives in their living area.
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        The SBGN representation was convenient to elaborate the Ordinary Differential Equations (ODEs) system, which is our second approach. Starting with a complex system, choices have been made to simplify some molecular cascades or some interactions to reduce it to a system of 12 differential equations.
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          <a href="http://www.20minutes.fr/toulouse/2123171-20170829-toulouse-etudiants-imitent-crocodile-attaquer-cholera">Click here to read the full article (in french)</a>
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        <i>Cot&eacute; Toulouse</i> is a weekly free paper aiming to deliver all the local information. Once again we were pleased to reach at the local inhabitants of Toulouse to show them what the students of their city are doing and to mobilize them around the project.
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          <a href="https://actu.fr/occitanie/toulouse_31555/a-toulouse-etudiants-travaillent-sur-une-molecule-crocodile-lutter-contre-cholera_11690457.html">Click here to read the full article (in french) </a>
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        <i>La d&eacute;p&ecirc;che du midi</i> is a regional daily newspaper sold in approximately 150,000 copies everyday. Thus it reaches a wide public living in a large area. This publication was an opportunity to make this audience discover our project and to tickle their curiosity to learn more.
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          <a href="https://www.ladepeche.fr/article/2017/09/01/2637267-neuf-etudiants-travaillent-crocodile-lutter-contre-cholera.html">Click here to read the full article (in french) </a>
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        After being interviewed by local and regional newspaper we were thrilled that <i>Le Journal du Dimanche</i> published an article about our project. Indeed it is a weekly national newspaper reaching around 200,000 people. This article obviously gave our team a national exposure, along with synthetic biology and the iGEM competition. We hope that this article tickled the curiosity of many french people.
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          <a href="http://www.lejdd.fr/societe/sciences/pourquoi-le-crocodile-resiste-au-cholera-3430838">Click here to read the full article (in french) </a>
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        <i>Aujourd&rsquo;hui en France</i> is a national weekly newspaper gathering almost 140,000 readers every day. It emphasis the interesting regional initiatives in France, giving them more exposure. We were thus delighted to be part of these noteworthy projects.
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          <a href="http://www.leparisien.fr/vie-quotidienne/sante/les-crocodiles-armes-anticholera-05-09-2017-7235709.php">Click here to read the full article (in french) </a>
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        Our project was also presented in the online version of France info which is a french radio. Some members of the team were interviewed by the journalist and it was a good practice to present our project in a popularized way with our words.
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          <a href="http://www.francetvinfo.fr/sante/decouverte-scientifique/pour-lutter-contre-le-cholera-des-etudiants-chercheurs-francais-misent-sur-le-crocodile_2364949.html">Click here to read &amp; listen the full article (in french) </a>
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Revision as of 12:22, 24 September 2017

Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Model overview

Aims of the model

Our strategy was based on an input with a quorum sensing molecule production, a molecular communication between two organisms, and an output with the antimicrobial peptides effect. We needed to use a model to simulate this biological system and analyze if this communication was feasible:

Would the quorum sensing molecule (CAI-1) induce a sufficient answer to activate the sensor (Vibrio harveyi)? Would the receptor be able to produce enough molecular message (diacetyl) to communicate with the effector Pichia pastoris? Would the effector produce enough antimicrobial peptides to deliver the guessed output, which is the lysis of V. cholerae to reach a non-toxic concentration?

A model was also crucial regarding the entrepreneurship and the integrated human practices parts of our project: we needed to show to clients and investors, but also to citizens, how our system would work, how we will dimensionate our device, and how long do you have to wait before drinking a non-contaminated water.

To sum up, the goals of our model could be sum up into four objectives:

  • Demonstrate the feasibility
  • Confirm our wet lab strategy
  • Dimensionate
  • Estimate the waiting time

Approaches

Two complementary approaches have been used to represent our model. Working with a complex biological system involving three microorganisms and several molecules, the Systemic Biology Graphical Notation (SBGN) was a perfect way to ordinate the system and represent the interactions between organisms and the molecules involved.

The SBGN representation was convenient to elaborate the Ordinary Differential Equations (ODEs) system, which is our second approach. Starting with a complex system, choices have been made to simplify some molecular cascades or some interactions to reduce it to a system of 12 differential equations.