Difference between revisions of "Team:Munich"

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Revision as of 10:04, 26 September 2017

Description

Design

Proof of Concept

Demonstration

Application

Final Results

Entrepeneurship

Achievements

Protocols and
Methods

Materials

LabJournal

Safety

Parts

Interlab

Software

Hardware

Human Practices (Silver)

Human Practices (Gold)

Public Engagement

Collaborations

Team members

Supervisors

PIs

Institutions

Sponsors

Attributions

Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.

Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

| The iGEM Munich 2017 Team | 2017.07.13 |