Difference between revisions of "Team:JNFLS/Interlab"

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<tr><td>Arith. Std.Dev.</td><td>33733.71</td><td>32246.67</td><td>19441.13</td><td>10303.09</td><td>7043.812</td><td>4260.032</td><td>1850.053</td><td>1105.718</td><td>643.4931</td><td>276.7735</td><td>286.5593</td><td>33.61919</td></tr>
 
<tr><td>Arith. Std.Dev.</td><td>33733.71</td><td>32246.67</td><td>19441.13</td><td>10303.09</td><td>7043.812</td><td>4260.032</td><td>1850.053</td><td>1105.718</td><td>643.4931</td><td>276.7735</td><td>286.5593</td><td>33.61919</td></tr>
 
</table>
 
</table>
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<img src="/igem/images/chart-1.png"/>
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<img src="https://static.igem.org/mediawiki/2017/b/b0/JNFLS-chart_1.png"/>
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<img src="/igem/images/chart-2.png"/>
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<img src="https://static.igem.org/mediawiki/2017/b/b2/JNFLS-chart_2.png"/>
 
<h4>Normalization</h4>
 
<h4>Normalization</h4>
 
<p>Table 3. Normalization.</p>
 
<p>Table 3. Normalization.</p>
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<tr><th>Hour 6:</th><td>0.276</td><td>0.222225</td><td>0.142525</td><td>0.29355</td><td>0.066925</td><td>0.293825</td><td>0.30975</td><td>0.14125</td><td>0.51715</td><td>0.3028</td><td>0.236725</td><td>0.10015</td><td>0.29385</td><td>0.218025</td><td>0.225175</td><td>0.2391</td></tr>
 
<tr><th>Hour 6:</th><td>0.276</td><td>0.222225</td><td>0.142525</td><td>0.29355</td><td>0.066925</td><td>0.293825</td><td>0.30975</td><td>0.14125</td><td>0.51715</td><td>0.3028</td><td>0.236725</td><td>0.10015</td><td>0.29385</td><td>0.218025</td><td>0.225175</td><td>0.2391</td></tr>
 
</table>
 
</table>
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<img src="/igem/images/chart-3.png"/>
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<img src="https://static.igem.org/mediawiki/2017/a/a9/JNFLS-chart_3.png"/>
 
<p>Figure 3. Blank subtraction and correction of Abs600 measurement.</p>
 
<p>Figure 3. Blank subtraction and correction of Abs600 measurement.</p>
 
<p>Table 6. Raw data of fluorescence measurement.</p>
 
<p>Table 6. Raw data of fluorescence measurement.</p>
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<tr><th>Hour 6:</th><td>2748.5</td><td>2392.5</td><td>15685.5</td><td>28219.25</td><td>3164.75</td><td>15852.25</td><td>27159.75</td><td>11163.75</td><td>6200.25</td><td>3457.5</td><td>21030.25</td><td>5821.5</td><td>5481.25</td><td>4656</td><td>3194.75</td><td>3078.75</td></tr>
 
<tr><th>Hour 6:</th><td>2748.5</td><td>2392.5</td><td>15685.5</td><td>28219.25</td><td>3164.75</td><td>15852.25</td><td>27159.75</td><td>11163.75</td><td>6200.25</td><td>3457.5</td><td>21030.25</td><td>5821.5</td><td>5481.25</td><td>4656</td><td>3194.75</td><td>3078.75</td></tr>
 
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</table class="medium">
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<img src="/igem/images/chart-4.png"/>
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<p>Figure 4. Blank subtraction and correction of fluorescence measurement.</p>
 
<p>Figure 4. Blank subtraction and correction of fluorescence measurement.</p>
 
<p>Table 8.Raw data of Fl/Abs600.</p>
 
<p>Table 8.Raw data of Fl/Abs600.</p>
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</table>
 
</table>
 
<p>Figure 5. Average level of devices.</p>
 
<p>Figure 5. Average level of devices.</p>
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<img src="/igem/images/chart-5.png"/>
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</div>
 
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Revision as of 09:43, 28 September 2017

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JUDGING FORM

JNFLS

Interlab

Methods BackgroundMaterialsProtocol Result SequencingData

Methods

Background

The interlab is aimed to create reliable and repeatable measurement data and standardize the measurement of fluorescence in labs around the world by testing replicability of data, thus achieving to support the engineering of biological experiment. This academic community prevents the disadvantages of difference in experiment by providing a detailed protocol and data analysis method. Interlab has become one of the largest interlaboratory study worldwide in synthetic biology, hitherto. The data collected from the previous studies is summarized in a presentation iGEM 2016 Interlab Results Presentation. This time it is done by following the same protocols designed for a plate reader and a flow cytometer that will produce data in same units everywhere so it can be compared and analysed. Our team decided to test both protocols to measure the fluorescence.

Materials

Plasmids used

Positive Control Device: I20270 in pSB1C3 (Located in Kit Plate 6, well 20B)

Negative Control Device: R0040 in pSB1C3 (Located in Kit Plate 6, well 20D)

• Test Device 1: J23101+I13504 in pSB1C3 (Located in Kit Plate 6, well 20F)

• Test Device 2: J23106+I13504 in pSB1C3 (Located in Kit Plate 6, well 20H)

• Test Device 3: J23117+I13504 in pSB1C3 (Located in Kit Plate 6, well 20J)

• Test Device 4: J23101.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20L)

• Test Device 5: J23106.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20N)

• Test Device 6: J23117.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20P)

Strain used

• Escherichia coli​ strain DH5α

Materials

• 1ml LUDOX

• H​2​0

• 96 well plate

• fluorescein

• 10ml 1xPBS

• LB (Luria Bertani) media

• Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)

• 50 ml Falcon tube

• Incubator at 37°C

• 1.5 ml eppendorf tubes

• Ice bucket with ice

• Pipettes

Machines

• Thermo Multiskan FC

• BERTHOLD TECHNOLOGIES LB940Mith

Protocol

Calibration Protocols

Cell measurement protocol

Result

Sequencing

• Positive Control ：BBa_I20270

• Negative Control ：BBa_R0040

• Test Device 1 ：BBa_J364000

• Test Device 2 ：BBa_J364001

• Test Device 3 ：BBa_J364002

• Test Device 4 ：BBa_J364003

• Test Device 5 ：BBa_J364004

• Test Device 6 ：BBa_J364005

> BBa_I20270 Part-only sequence (919 bp)

ttgatggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

> BBa_R0040 Part-only sequence (54 bp)

tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac

> BBa_J364000 Part-only sequence (918 bp)

tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

> BBa_J364001 Part-only sequence (918 bp)

tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

> BBa_J364002 Part-only sequence (918 bp)

ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

> BBa_J364003 Part-only sequence (990 bp)

tttacagctagctcagtcctaggtattatgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

> BBa_J364004 Part-only sequence (990 bp)

tttacggctagctcagtcctaggtatagtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

> BBa_J364005 Part-only sequence (990 bp)

ttgacagctagctcagtcctagggattgtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

Data

OD600 Reference Point

Table 1. OD600 Reference Point.

LUDOX-HS40H2O
Replicate 10.04970.0483
Replicate 20.04930.0463
Replicate 30.05180.0492
Replicate 40.05160.047
Arith. Mean0.05060.0477
Corrected Abs6000.0026667
Reference OD6000.0425
OD600/Abs60015.9375

Fluorescein Standard Curve

Table 2. Data of Fluorescein Standard Curve.

uM Fluorescein50.002512.56.253.1251.56250.781250.3906250.1953130.0976560.0488280
Replicate 1193738610406805921613223791638198676542958222161173959253079444
Replicate 2185945311157876177593160511580968208142161215091105258543048444
Replicate 3187490610992746116123272651569257780140314196421025554302655373
Replicate 4189395310829736393593403461723488657344775215401144660763351414
Arith. Mean18914251084679615222.8326510.3162797833054255221226.75111235821.253033.25418.75
Arith. Std.Dev.33733.7132246.6719441.1310303.097043.8124260.0321850.0531105.718643.4931276.7735286.559333.61919

Normalization

Table 3. Normalization.

sampleAbs600 ReadingVolume of Preloading CultureVolume of Preloading Media
positive control0.0906256.4412238333.558776167
negative control0.09858755.1265619994.873438001
device 10.097855.2253429134.774657087
device 20.101054.8221820375.177817963
device 30.1031754.5871559635.412844037
device 40.09873755.1069262694.893073731
device 50.11836253.4020837766.597916224
device 60.09855.1380860634.861913937
media+chl0.059575

Cell Measurement

Table 4. Raw data of Abs600 measurement.

Abs600 Raw Readings:Neg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6LB + Chlor (blank)
Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2
Hour 0:0.091550.08970.0903250.106850.1048750.0908250.096550.105550.10960.096750.1070250.090450.130850.1058750.10690.09010.06640.059575
Hour 2:0.17930.18550.103250.163250.105250.113650.175450.150350.2028750.1848250.14970.10190.197250.1774750.18770.1608250.06830.05675
Hour 4:0.24210.2465750.1341750.2494250.1172250.2080750.239850.1618750.42470.258650.2305250.1240250.255950.2409250.2442250.246650.068150.06885
Hour 6:0.34240.2886250.2089250.359950.1333250.3602250.376150.207650.583550.36920.3031250.166550.360250.2844250.2915750.30550.07790.064775

Table 5. Blank subtraction and correction of Abs600 measurement.

OD600-BackgroundNeg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6
Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2
Hour 0:0.025150.02330.0239250.040450.0384750.0244250.030150.039150.04320.030350.0406250.024050.064450.0394750.04050.0237
Hour 2:0.11290.11910.036850.096850.038850.047250.109050.083950.1364750.1184250.08330.03550.130850.1110750.12130.094425
Hour 4:0.17570.1801750.0677750.1830250.0508250.1416750.173450.0954750.35830.192250.1641250.0576250.189550.1745250.1778250.18025
Hour 6:0.2760.2222250.1425250.293550.0669250.2938250.309750.141250.517150.30280.2367250.100150.293850.2180250.2251750.2391

Figure 3. Blank subtraction and correction of Abs600 measurement.

Table 6. Raw data of fluorescence measurement.

Raw fluorescenceNeg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6LB + Chlor (blank)
Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2
Hour 0:11978.7512054.2513919.2514055.2512311.513868.517252.514736.7512430.2512286.514287.513227.512238.2512178.2511854.512076.7511317.2511138.25
Hour 2:1256412755.751665222236.515541.515458.524010.2517610.7513101.512591.7520517.751423214340.514186.512589.512354.7510681.7510787.5
Hour 4:13356.2513479.7521582.7531126.2513257.7520684.2528890.518862.251483513921.528713.515357.2515024.515387.513588.513468.2511143.2511640.5
Hour 6:14065.7513709.7527002.7539536.51448227169.5384772248117517.514774.7532347.517138.7516798.515973.25145121439611572.2511271.75

Table 7. Blank substraction and correction of fluorescence measurement.

fluorescence-BackgroundNeg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6
Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2
Hour 0:661.573726022738994.252551.255935.253419.51113969.252970.251910.25921861537.25759.5
Hour 2:1246.751438.55334.7510919.254224.254141.25126936293.51784.251274.59200.52914.753023.252869.251272.251037.5
Hour 4:20392162.510265.5198091940.5936717573.2575453517.752604.2517396.2540403707.254070.252271.252151
Hour 6:2748.52392.515685.528219.253164.7515852.2527159.7511163.756200.253457.521030.255821.55481.2546563194.753078.75

Figure 4. Blank subtraction and correction of fluorescence measurement.

Table 8.Raw data of Fl/Abs600.

FI/OD600Neg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6
Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2Colony 1Colony 2
Hour 0:0.03636560.04368940.16109030.10468720.03716170.24343950.31123880.12240770.03517090.04595740.10340990.11364990.02575670.02917390.02073880.0473831
Hour 2:0.0150150.01655080.19930330.15552740.16296870.13444710.16127640.10368440.01785520.01492480.15590020.11617020.03350340.03544440.01410510.0152842
Hour 4:0.01591760.0164590.20813840.14849840.05373760.09176840.13921040.10856670.01345810.0185530.14570910.09723470.02762450.03206850.0175510.0165957
Hour 6:0.01365990.0147320.16500950.13178190.06556060.07399850.12576050.1084650.01632160.0167740.12360020.07958520.02572590.02961430.01930780.017636

Table 9. Raw data of average

Raw data of averageNeg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6
Hour 0:0.040027520.1328887960.1403006080.2168232750.0405641660.1085298720.0274653110.034060932
Hour 2:0.0157829220.1774153620.1487078960.1324804150.0163900080.1085298720.0344739020.014694671
Hour 4:0.0161883080.1783184190.0727530210.1238885420.0160055590.1085298720.0298465060.01707336
Hour 6:0.0141959160.1483956790.0697795190.1171127470.0165478030.1085298720.0276700750.018471905

Table 10. Raw data of SD

Raw data of SDNeg. ControlPos. ControlDevice 1Device 2Device 3Device 4Device 5Device 6
Hour 0:0.0150795490.051863290.1078499120.078215610.0083730590.024795480.0089973790.021020162
Hour 2:0.0024644550.0118655980.0475506270.0146233250.003379950.0295219580.0054926790.003828995
Hour 4:0.0013575210.0095550020.0125886670.0061611790.002022540.0114693460.0041597370.002278645
Hour 6:0.0020691220.0292154620.0054244710.0122719260.0030211930.006552860.0030272560.002185333

Figure 5. Average level of devices.