Rstclair2012 (Talk | contribs) |
Rstclair2012 (Talk | contribs) |
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<center><h4>Methodology</h4></center> | <center><h4>Methodology</h4></center> | ||
<h5>Materials</h5> | <h5>Materials</h5> | ||
− | Competent cells (Escherichia coli strain DH5α) | + | ● Competent cells (Escherichia coli strain DH5α) |
<br/> | <br/> | ||
− | LB (Luria Bertani) media | + | ● LB (Luria Bertani) media |
<br/> | <br/> | ||
− | Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL) | + | ● Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL) |
<br/> | <br/> | ||
− | 50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light) | + | ● 50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light) |
<br/> | <br/> | ||
− | Incubator at 37°C | + | ● Incubator at 37°C |
<br/> | <br/> | ||
− | 1.5 ml eppendorf tubes for sample storage | + | ● 1.5 ml eppendorf tubes for sample storage |
<br/> | <br/> | ||
− | Ice bucket with ice | + | ● Ice bucket with ice |
<br/> | <br/> | ||
− | Pipettes | + | ● Pipettes |
<br/> | <br/> | ||
− | 96 well plate, 2 different plates used: clear with flat bottom for absorbance; black with flat bottom for fluorescence | + | ● 96 well plate, 2 different plates used: clear with flat bottom for absorbance; black with flat bottom for fluorescence |
<br/> | <br/> | ||
Revision as of 22:10, 29 September 2017
Florida_Atlantic