Difference between revisions of "Team:KUAS Korea/Demonstrate"

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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <h3>Demonstrate</h3>
 
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<h2>Demonstrate</h2><br>
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<p><font size=4px>Our project is about creating an Enzymatic Microbial Fuel cell that uses agar as its energy source. We designed the battery device this way. </p>
  
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<h1>Demonstrate</h1>
 
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<h3>Gold Medal Criterion #4</h3>
 
  
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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<p><font size=4px>First, <em>E.coli</em> that displays agar degrading enzymes degrade agar to produce galactose and NADH. Galactose is then used by <em>E.coli</em> to produce lactate and formate which <em>shewanella oneidensis</em> can use to generate electricity. NADH is used by diaphorase to generate electricity as well.</p><Br>
  
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
 
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<p><font size=4px>This is how we made the prototype. </p>
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<p><font size=4px><em>Shewanella oneidensis</em>MR-1, BW25113 with displayed agar degrading enzymes, cell lysate of diaphorase expressed BL21(DE3), and cell lysate of TEV expressed BL21(DE3) was put into the anode chamber. (The function of TEV is explained in the Experiment section.) Agar was used as the substrate. The battery device without agar was set as the control.</p><Br>
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                        <img src=" https://static.igem.org/mediawiki/2016/e/eb/Korea_U_Seoul_EMFC.jpeg">
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<p><font size=4px>As you can see in the graph above, the battery with agar generated more electricity than the control. The voltages the control generate is nearly 0, while our prototype EMFC generates 0.1V of electricity. Our prototype EMFC apparently works as expected. </p><Br>
  
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<p><font size=4px>However, the voltage generated is not very high. This was expected due to some reasons. First, our battery device was not designed to generated high electricity. To get high yield, you need electrodes with large surface area but the electrodes of our device has small surface area since it is only a thin carbon paper with coated back. We designed our device this way for precise comparison. Second, our added diaphorase was in free state which means the majority of the diaphorase was not doing much to generate electricity. A lot of EFC relate theses fixes diaphorase onto the electrode for higher yield, and stabilization of the enzymes. </p><Br>
  
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<h4> What should we do for our demonstration?</h4>
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<p><font size=4px>Since we succeeded in operating the prototype EMFC we designed, our next goal is to improve electricity yield. This includes improving the battery device with better electrodes, optimizing the amount of reagents and cells in the device, and purifying diaphorase to get rid of useless materials.  </p></font><Br>
  
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<h5> Special track teams </h5>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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Latest revision as of 03:52, 2 October 2017

Demonstrate




Demonstrate


Our project is about creating an Enzymatic Microbial Fuel cell that uses agar as its energy source. We designed the battery device this way.

First, E.coli that displays agar degrading enzymes degrade agar to produce galactose and NADH. Galactose is then used by E.coli to produce lactate and formate which shewanella oneidensis can use to generate electricity. NADH is used by diaphorase to generate electricity as well.


This is how we made the prototype.

Shewanella oneidensisMR-1, BW25113 with displayed agar degrading enzymes, cell lysate of diaphorase expressed BL21(DE3), and cell lysate of TEV expressed BL21(DE3) was put into the anode chamber. (The function of TEV is explained in the Experiment section.) Agar was used as the substrate. The battery device without agar was set as the control.



As you can see in the graph above, the battery with agar generated more electricity than the control. The voltages the control generate is nearly 0, while our prototype EMFC generates 0.1V of electricity. Our prototype EMFC apparently works as expected.


However, the voltage generated is not very high. This was expected due to some reasons. First, our battery device was not designed to generated high electricity. To get high yield, you need electrodes with large surface area but the electrodes of our device has small surface area since it is only a thin carbon paper with coated back. We designed our device this way for precise comparison. Second, our added diaphorase was in free state which means the majority of the diaphorase was not doing much to generate electricity. A lot of EFC relate theses fixes diaphorase onto the electrode for higher yield, and stabilization of the enzymes.


Since we succeeded in operating the prototype EMFC we designed, our next goal is to improve electricity yield. This includes improving the battery device with better electrodes, optimizing the amount of reagents and cells in the device, and purifying diaphorase to get rid of useless materials.