Difference between revisions of "Team:KUAS Korea/Results"

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<div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2016/c/c1/Korea_U_Seoul_result.jpg)">
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    <h3>Results</h3>
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<h2>Results</h2><br>
  
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
 
  
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<h5>What should this page contain?</h5>
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<p><font size=4>All the graphs are measured voltages of our bio-batteries. The voltages were measured by  Keithley Digital Multimeter. The unit of X axis is hours measured.</font></p><br><br>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<h4>1. MFC Validation</h4><br>
  
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<p><font size=4>We measured the voltage that <em>Shewanella oneidensis</em> MR-1 produce when formate is given since MR-1 uses formate to generate electricity. As controls, we measured the voltage of the battery with <em>E. coli</em> BW25113 in the anode chamber, and the battery without microbes. Methylene blue was given as the mediator. The purpose of this experiment is to make sure that our battery device works as an MFC. </font></p><br>
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                        <img src=" https://static.igem.org/mediawiki/2016/e/ef/Korea_U_Seoul_MFC.jpeg " width=100%>
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<p><font size=4>As you can see in the graph above, the battery with <em>shewanella oneidensis</em> MR-1 generated electricity while the controls did not. This indicates that out battery device works as an MFC.</font></p>
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<h4>2. EFC Validation</h4><br>
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<p><font size=4>Diaphorase expressed <em>E. coli</em> BL21(DE3) was lysed by sonication and was applied into the anode chamber of our battery device. Methylene blue was given as the mediator. Lysed BL21(DE3) with void vector was the control. NADH was added to both batteries.          </font></p>          <br> 
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                        <img src="https://static.igem.org/mediawiki/2016/6/65/Korea_U_Seoul_EFC.jpeg" width=100%>
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<h5> Project Achievements </h5>
 
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<br><br><br>
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<p><font size=4>As you can see in the graph above, the battery with diaphorase showed higher voltages. This indicates that the diaphorase produced electricity. However, the voltage differences are very small. This could be due to the state of diaphorase, which was not fixed onto the electrode. Also it could be due to the components that exist in crude bacteria extract since there are many reducing or oxidizing agents in bacterial cells.</font> </p><br><br>
  
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h5>Inspiration</h5>
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<h4>3. EMFC Operation</h4><br>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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<p><font size=4>Our final goal was to prove that our device works as an EMFC. <em>Shewanella oneidensis</em> MR-1, BW25113 with displayed agar degrading enzymes, cell lysate of diaphorase expressed BL21(DE3), and cell lysate of TEV expressed BL21(DE3) was put into the anode chamber. Agar was used as the substrate. The battery device without agar was set as the control.  </font></p>    <br><br>           
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                        <img src=" https://static.igem.org/mediawiki/2016/e/eb/Korea_U_Seoul_EMFC.jpeg" width=100%>
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<br><br><br>
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<p><font size=4>As you can see in the graph above, the battery with agar generated more electricity than the control. Our prototype EMFC apparently works as expected. </font></p>
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<br><p><font size=4>However, the voltage generated is not very high. This was expected due to some reasons. First, our battery device was not designed to generated high electricity. To get high yield, you need electrodes with large surface area but the electrodes of our device has small surface area since it is only a thin carbon paper with coated back. We designed our device this way for precise comparisont. Second, our added diaphorase was in free state which means the majority of the diaphorase was not doing much to generate electricity. A lot of EFC relate theses fixes diaphorase onto the electrode for higher yield, and stabilization of the enzymes. </font></p>
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<br><p><font size=4>Since we succeeded in operating the prototype EMFC we designed, our next goal is to improve electricity yield. This includes improving the battery device with better electrodes, optimizing the amount of reagents and cells in the device, and purifying enzymes to get rid of uselss cell lysate.</font> </p>
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{{:Team:KUAS_Korea/Templates/Sponsors}}

Revision as of 11:33, 2 October 2017

Results

Results


All the graphs are measured voltages of our bio-batteries. The voltages were measured by Keithley Digital Multimeter. The unit of X axis is hours measured.



1. MFC Validation


We measured the voltage that Shewanella oneidensis MR-1 produce when formate is given since MR-1 uses formate to generate electricity. As controls, we measured the voltage of the battery with E. coli BW25113 in the anode chamber, and the battery without microbes. Methylene blue was given as the mediator. The purpose of this experiment is to make sure that our battery device works as an MFC.





As you can see in the graph above, the battery with shewanella oneidensis MR-1 generated electricity while the controls did not. This indicates that out battery device works as an MFC.



2. EFC Validation


Diaphorase expressed E. coli BL21(DE3) was lysed by sonication and was applied into the anode chamber of our battery device. Methylene blue was given as the mediator. Lysed BL21(DE3) with void vector was the control. NADH was added to both batteries.





As you can see in the graph above, the battery with diaphorase showed higher voltages. This indicates that the diaphorase produced electricity. However, the voltage differences are very small. This could be due to the state of diaphorase, which was not fixed onto the electrode. Also it could be due to the components that exist in crude bacteria extract since there are many reducing or oxidizing agents in bacterial cells.



3. EMFC Operation


Our final goal was to prove that our device works as an EMFC. Shewanella oneidensis MR-1, BW25113 with displayed agar degrading enzymes, cell lysate of diaphorase expressed BL21(DE3), and cell lysate of TEV expressed BL21(DE3) was put into the anode chamber. Agar was used as the substrate. The battery device without agar was set as the control.






As you can see in the graph above, the battery with agar generated more electricity than the control. Our prototype EMFC apparently works as expected.


However, the voltage generated is not very high. This was expected due to some reasons. First, our battery device was not designed to generated high electricity. To get high yield, you need electrodes with large surface area but the electrodes of our device has small surface area since it is only a thin carbon paper with coated back. We designed our device this way for precise comparisont. Second, our added diaphorase was in free state which means the majority of the diaphorase was not doing much to generate electricity. A lot of EFC relate theses fixes diaphorase onto the electrode for higher yield, and stabilization of the enzymes.


Since we succeeded in operating the prototype EMFC we designed, our next goal is to improve electricity yield. This includes improving the battery device with better electrodes, optimizing the amount of reagents and cells in the device, and purifying enzymes to get rid of uselss cell lysate.