Difference between revisions of "Team:INSA-UPS France/test/description"

Line 131: Line 131:
 
</section>
 
</section>
  
 +
<section>
 +
<h1>We are able to mimic vibrios quorum sensing molecules</h1>
 +
<p>
 +
CAI-1 like molecule are crucial to start our system and gather the information of the presence of high quantity of <i>Vibrio cholerae</i>, here we prove with mass spectrometry (MS) and nuclear magnetic resonance (NMR) approaches that we are able to produce C8-CAI-1 using <i>E.coli</i>.
 +
</p>
 +
</section>
  
 +
<section>
 +
<h1><i>Vibrio harveyi</i> JMH626 is able to detect CAI-1 like molecules </h1>
 +
<p>
 +
Based on our C8-CAI-1 production, we induced quorum sensing pathway ending in bioluminesnce in <i>V. harveyi</i> JMH626 using a self made protocol.
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2017/1/13/T--INSA-UPS_France--Results_Coli_biolum.png" alt="">
 +
</section>
 +
 +
<section>
 +
<h1><i>Vibrio harveyi</i> can be used a molecular chassis</h1>
 +
<p>
 +
Using <i>Vibrio harveyi</i> and inserting iGEM biobrik through conjugaison has been successfully made with the insertion of RFP.
 +
 +
</p>
 +
</section>
 +
 +
<section>
 +
<h1>Core discussion between prokariotic and eukaryotic is on the way</h1>
 +
<p>
 +
We found a common media where our two microorganisms were able to growth, leading to possible co-culture and further communications.
 +
</p>
 +
</section>
 +
 +
<section>
 +
<h1><i>Pichia pastoris</i> is able to produce functional antimicrobial peptides</h1>
 +
<p>
 +
We show that <i>Pichia pastoris</i> is able to produce antimicrobial peptdides that are able to inhibit <i>Vibrio harveyi</i> growth on plate.
 +
</p>
 +
</section>
 
      
 
      
 
     <!-- fin section -->     
 
     <!-- fin section -->     

Revision as of 20:15, 6 October 2017


Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Results

We are able to mimic vibrios quorum sensing molecules

CAI-1 like molecule are crucial to start our system and gather the information of the presence of high quantity of Vibrio cholerae, here we prove with mass spectrometry (MS) and nuclear magnetic resonance (NMR) approaches that we are able to produce C8-CAI-1 using E.coli.

Vibrio harveyi JMH626 is able to detect CAI-1 like molecules

Based on our C8-CAI-1 production, we induced quorum sensing pathway ending in bioluminesnce in V. harveyi JMH626 using a self made protocol.

Vibrio harveyi can be used a molecular chassis

Using Vibrio harveyi and inserting iGEM biobrik through conjugaison has been successfully made with the insertion of RFP.

Core discussion between prokariotic and eukaryotic is on the way

We found a common media where our two microorganisms were able to growth, leading to possible co-culture and further communications.

Pichia pastoris is able to produce functional antimicrobial peptides

We show that Pichia pastoris is able to produce antimicrobial peptdides that are able to inhibit Vibrio harveyi growth on plate.