Difference between revisions of "Team:INSA-UPS France/test/description"

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       border-bottom: solid 3px #0097a7 !important; /* bleuvert */
 
       border-bottom: solid 3px #0097a7 !important; /* bleuvert */
 +
    }
 +
    .week-experiment{
 +
      border:solid 5px #0097a7;
 
     }
 
     }
  
Line 193: Line 196:
 
       <h3>January</h3>
 
       <h3>January</h3>
 
       <table>
 
       <table>
−
         <tr data-target="1" class="current-week">
+
         <tr data-target="1" >
 
           <td>1</td>
 
           <td>1</td>
 
           <td>2</td>
 
           <td>2</td>
Line 220: Line 223:
 
           <td>21</td>
 
           <td>21</td>
 
         </tr>
 
         </tr>
−
         <tr data-target="4">
+
         <tr data-target="4" class="current-week">
 
           <td>22</td>
 
           <td>22</td>
 
           <td>23</td>
 
           <td>23</td>
Line 938: Line 941:
 
       <section class="week week-team">
 
       <section class="week week-team">
 
         <h2>03-05: Team building at the restaurant</h2>
 
         <h2>03-05: Team building at the restaurant</h2>
 +
        <p>
 +
          Our team met for the first time outside the INSA buildings and shared a delicious korean meal after brainstorming the whole afternoon!
 +
        </p>
 
         <img src="https://static.igem.org/mediawiki/2017/6/60/T--INSA-UPS_France--Notebook_Week-9.jpg" alt="">
 
         <img src="https://static.igem.org/mediawiki/2017/6/60/T--INSA-UPS_France--Notebook_Week-9.jpg" alt="">
 
       </section>
 
       </section>
 
     </div>
 
     </div>
−
<!-- *************************************************************** -->
 
  
−
     <section class="week" id="week-10">
+
     <!-- Week 10 -->
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 10 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <div class="week" id="week-10">
−
       <h2>03-09: 7th brainstorming meeting</h2>
+
       <section>
−
      <p><i>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 10 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
        Dropping of first device
+
       </section>
−
      </i></p>
+
      <section class="week-meeting">
−
    </section>
+
        <h2>03-09: 7th brainstorming meeting</h2>
−
    <section class="week" id="week-11">
+
        <p><i>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 11 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
          Dropping of first device
−
       <h2></h2>
+
        </i></p>
−
    </section>
+
      </section>
−
     <section class="week" id="week-12">
+
      <section class="week-team">
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 12 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
        <h2>03-12: Brainstorming in a caf&eacute;</h2>
−
       <h2>03-25: Team Building</h2>
+
        <img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Notebook_Week-10.jpg" alt="">
−
       <p>
+
      </section>
−
        Our team met for the first time outside the INSA buildings and shared a delicious korean meal after brainstorming the whole afternoon !
+
    </div>
−
      </p>
+
 
−
      <img src="https://static.igem.org/mediawiki/2017/9/9f/T--INSA-UPS_France--Notebook_Week-12_1.jpg" alt="">
+
    <!-- Week 11 -->
−
       <img src="https://static.igem.org/mediawiki/2017/5/54/T--INSA-UPS_France--Notebook_Week-12_2.jpg" alt="">
+
    <div class="week" id="week-11">
−
     </section>
+
       <section>
−
    <section class="week" id="week-13">
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 11 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 13 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
       </section>
−
       <h2>03-30: 10th brainstorming meeting</h2>
+
      <section class="week-meeting">
−
      <p><i>
+
        <h2>03-14: 8th brainstorming</h2>
−
        Technical solutions
+
      </section>
−
      </i></p>
+
     </div>
−
    </section>
+
 
−
     <section class="week" id="week-14">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 14 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <!-- Week 12 -->
−
       <h2>04-06: 12th brainstorming meeting</h2>
+
    <div class="week" id="week-12">
−
      <p><i>
+
       <section>
−
        Second device proposition
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 12 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </i></p>
+
       </section>
−
    </section>
+
      <section class="week-meeting">
−
    <section class="week" id="week-15">
+
        <h2>03-21: 9th brainstorming</h2>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 15 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
       </section>
−
    </section>
+
      <section class="week-team">
−
    <section class="week" id="week-16">
+
        <h2>03-26: Brainstorming in a caf&eacute; </h2>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 16 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
        <img src="https://static.igem.org/mediawiki/2017/f/fa/T--INSA-UPS_France--Notebook_Week-12.jpg" alt="">
−
       <h2>04-19: Meeting with Marc Lemonnier, CEO of Antabio</h2>
+
       </section>
−
      <p>
+
 
−
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a1">his testimony here.</a>
+
    <!-- Week 13 -->
−
      </p>
+
     <div class="week" id="week-13">
−
    </section>
+
       <section>
−
    <section class="week" id="week-17">
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 13 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 17 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
       </section>
−
    </section>
+
      <section class="week-meeting">
−
    <section class="week" id="week-18">
+
        <h2>03-30: 10th brainstorming</h2>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 18 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
        <p><i>
−
    </section>
+
          Technical solutions
−
    <section class="week" id="week-19">
+
        </i></p>
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 19 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
      </section>
−
       <h2>05-13: Paul &amp; Margaux joined our team</h2>
+
     </div>
−
      <img src="https://static.igem.org/mediawiki/2017/6/6a/T--INSA-UPS_France--Notebook_Week-19_1.jpg" alt="">
+
 
−
    </section>
+
 
−
    <section class="week" id="week-20">
+
    <!-- Week 14 -->
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 20 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <div class="week" id="week-14">
−
       <h2>05-18: iGEM distribution kit received</h2>
+
       <section>
−
      <img src="https://static.igem.org/mediawiki/2017/7/70/T--INSA-UPS_France--Notebook_Week-20.jpg" alt="">
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 14 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
    </section>
+
       </section>
−
     <section class="week" id="week-21">
+
      <section class="week-meeting">
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 21 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
        <h2>04-06: 11th brainstorming</h2>
−
       <h2>05-25: Parts design day</h2>
+
        <p><i>
−
      <!-- text -->
+
          Second device proposition
−
      <img src="https://static.igem.org/mediawiki/2017/f/f0/T--INSA-UPS_France--Notebook_Week-21_2.jpg" alt="">
+
        </i></p>
−
    </section>
+
      </section>
−
     <section class="week" id="week-22">
+
      <section class="week-team">
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 22 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
        <h2>04-07: choice of the subject</h2>
−
       <h2>05-28: Official announcement of our subject</h2>
+
      </section>
−
      <p>
+
    </div>
−
        We officialy the subject of our project on social media!
+
 
−
      </p>
+
 
−
       <h2>05-31: Exposciences</h2>
+
    <!-- Week 15 -->
−
      <p>
+
    <div class="week" id="week-15">
−
        We took part to the Exposciences event.
+
       <section>
−
        <!-- LINK -->
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 15 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </p>
+
      </section>
−
      <h2>Experiments</h2>
+
      <section class="week-meeting">
−
      <div class="wet-lab-notebook">
+
        <h2>04-13: 12th brainstorming</h2>
−
        <div>
+
      </section>
−
           <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
    </div>
−
           <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
 
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
 
−
         </div>
+
    <!-- Week 16 -->
−
         <div>
+
    <div class="week" id="week-16">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 16 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
       </section>
 +
      <section class="week-ihp">
 +
        <h2>04-19: Meeting with Marc Lemonnier, CEO of Antabio</h2>
 +
        <p>
 +
          You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a1">his testimony here.</a>
 +
        </p>
 +
      </section>
 +
      <section class="week-meeting">
 +
        <h2>04-20: 13th brainstorming</h2>
 +
      </section>
 +
      <section class="week-team">
 +
        <h2>04-23: First drafts of Sobki</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/0/02/T--INSA-UPS_France--Notebook_Week-16.jpg" alt="">
 +
      </section>
 +
    </div>
 +
 
 +
    <!-- Week 17 -->
 +
    <div class="week" id="week-17">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 17 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      </section>
 +
      <section class="week-meeting">
 +
        <h2>04-26: 14th brainstorming</h2>
 +
      </section>
 +
    </div>
 +
 
 +
    <!-- Week 18 -->
 +
    <div class="week" id="week-18">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 18 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      </section>
 +
      <section class="week-meeting">
 +
        <h2>05-02: 15th brainstorming</h2>
 +
      </section>
 +
    </div>
 +
 
 +
 
 +
    <!-- Week 19 -->
 +
    <div class="week" id="week-19">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 19 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
       </section>
 +
      <section class="week-team">
 +
        <h2>05-13: Paul &amp; Margaux officialy joined our team!</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/6/6a/T--INSA-UPS_France--Notebook_Week-19_1.jpg" alt="">
 +
      </section>
 +
      <section class="week-meeting">
 +
        <h2>05-11: 16th brainstorming</h2>
 +
      </section>
 +
    </div>
 +
 
 +
    <!-- Week 20 -->
 +
    <div class="week" id="week-20">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 20 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
       </section>
 +
      <section class="week-meeting">
 +
        <h2>05-17: 17th brainstorming</h2>
 +
      </section>
 +
      <section class="week-experiment">
 +
        <h2>05-18: iGEM distribution kit received</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/7/70/T--INSA-UPS_France--Notebook_Week-20.jpg" alt="">
 +
      </section>
 +
     </div>
 +
 
 +
    <!-- Week 21 -->
 +
    <div class="week" id="week-21">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 21 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
       </section>
 +
      <section class="week-team">
 +
        <h2>05-25: Parts design day at Brice's</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/f/f0/T--INSA-UPS_France--Notebook_Week-21_2.jpg" alt="">
 +
      </section>
 +
     </div>
 +
 
 +
    <!-- Week 22 -->
 +
    <div class="week" id="week-22">
 +
       <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 22 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
       </section>
 +
      <section class="week-team">
 +
        <h2>05-28: Official announcement of our subject</h2>
 +
        <p>
 +
          We officialy the subject of our project on social media!
 +
        </p>
 +
       </section>
 +
      <section class="week-pe">
 +
        <h2>05-31: Exposciences</h2>
 +
        <p>
 +
          We took part to the Exposciences event.
 +
          <!-- LINK -->
 +
           <img src="https://static.igem.org/mediawiki/2017/f/fb/T--INSA-UPS_France--Engagement-Exposciences_1.jpg" alt="">
 +
        </p>
 +
        <h2>06-01: Interventions in schools</h2>
 +
        <p>
 +
           <!-- LINK -->
 +
          <img src="https://static.igem.org/mediawiki/2017/6/61/T--INSA-UPS_France--Notebook_Week-22.jpg" alt="">
 +
        </p>
 +
      </section>
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
         <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Design and orders</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <ul>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Design of the gBlocks and launching of the synthesis order for IDT</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Design of the PCR oligos</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Design and orders</h2>
+
             <div>
−
            <ul>
+
              <h2>Design and orders</h2>
−
              <li>Design of the gBlocks and launching of the synthesis order for IDT</li>
+
              <ul>
−
              <li>Design of the PCR oligos</li>
+
                <li>Design of the gBlocks and launching of the synthesis order for IDT</li>
−
            </ul>
+
                <li>Design of the PCR oligos</li>
−
          </div>
+
              </ul>
−
          <div>
+
            </div>
−
             <!--<h2>Titre</h2>
+
            <div>
−
            <p>
+
              <h2>Design and orders</h2>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
              <ul>
−
            </p>-->
+
                <li>Design of the gBlocks and launching of the synthesis order for IDT</li>
 +
                <li>Design of the PCR oligos</li>
 +
              </ul>
 +
             </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-23">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 23 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 23 -->
−
       <h2>06-06: Meeting with the CEO of Sunwaterlife</h2>
+
    <div class="week" id="week-23">
−
      <p>
+
       <section>
−
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 23 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </p>
+
       </section>
−
       <h2>Experiments</h2>
+
      <section class="week-ihp">
−
       <div class="wet-lab-notebook">
+
        <h2>06-06: Meeting with the CEO of Sunwaterlife</h2>
−
         <div>
+
        <p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
          You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        </p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       </section>
−
         </div>
+
       <section class="week-meeting">
−
         <div>
+
         <h2>06-08: 18th brainstorming</h2>
 +
      </section>
 +
      <section class="week-pe">
 +
        <h2>06-09: Intervention in school</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/6/60/T--INSA-UPS_France--Engagement_school1.JPG" alt="">
 +
      </section>
 +
     
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
         <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <!--<h2>Titre</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <p>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </p>-->
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Taking care of <i>V. harveyi</i></h2>
+
             <div>
−
            <ul>
+
              <!--<h2>Titre</h2>
−
              <li>Reception and storage of <i>V. harveyi</i> BB120 (WT) and <i>V. harveyi</i> JMH626 (&Delta;cqsA &Delta;luxQ &Delta;luxN) CmR at -80 &deg;C.</li>
+
              <p>
−
              <li>Investigation of <i>V. harveyi</i> antibiotics resistances.</li>
+
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
−
            </ul>
+
              </p>-->
−
          </div>
+
            </div>
−
          <div>
+
            <div>
−
            <!--<h2>Titre</h2>
+
              <h2>Taking care of <i>V. harveyi</i></h2>
−
            <p>
+
              <ul>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Reception and storage of <i>V. harveyi</i> BB120 (WT) and <i>V. harveyi</i> JMH626 (&Delta;cqsA &Delta;luxQ &Delta;luxN) CmR at -80 &deg;C.</li>
−
            </p>-->
+
                <li>Investigation of <i>V. harveyi</i> antibiotics resistances.</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-24">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 24 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 24 -->
−
       <h2>06-15: Scope statement start</h2>
+
    <div class="week" id="week-24">
−
      <p><i>
+
       <section>
−
        Third device proposition
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 24 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </i></p>
+
       </section>
−
       <h2>Experiments</h2>
+
      <section class="week-ihp">
−
       <div class="wet-lab-notebook">
+
        <h2>06-14: Scope statement start</h2>
−
         <div>
+
        <p><i>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
          Third device proposition
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        </i></p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       </section>
−
         </div>
+
       <section class="week-meeting">
−
         <div>
+
         <h2>06-15: 19th brainstorming</h2>
 +
      </section>
 +
      <section class="week-igemers">
 +
        06-16: Skype with Greece iGEM team
 +
      </section>
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
         <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Amplifying pSB1C3</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <ul>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Reception of IDT gBlocks and storage</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Preparative work with the cloning vectors : Amplification and stock of pSB1C3</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Taking charge of the lab and of <i>V. harveyi</i></h2>
+
             <div>
−
            <ul>
+
              <h2>Amplifying pSB1C3</h2>
−
              <li>Growth kinetic assay in order to determine the adequate NaCl concentration for <i>V. harveyi</i> growth. </li>
+
              <ul>
−
              <li>Reception of IDT gBlocks and storage</li>
+
                <li>Reception of IDT gBlocks and storage</li>
−
              <li>Transformation of DH5&alpha; competent cells with pYFP, pDsRed, pPIZ&alpha;, pBR322 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
+
                <li>Preparative work with the cloning vectors : Amplification and stock of pSB1C3</li>
−
            </ul>
+
              </ul>
−
          </div>
+
            </div>
−
          <div>
+
            <div>
−
            <!--<h2>Titre</h2>
+
              <h2>Taking charge of the lab and of <i>V. harveyi</i></h2>
−
            <p>
+
              <ul>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Growth kinetic assay in order to determine the adequate NaCl concentration for <i>V. harveyi</i> growth. </li>
−
            </p>-->
+
                <li>Reception of IDT gBlocks and storage</li>
 +
                <li>Transformation of DH5&alpha; competent cells with pYFP, pDsRed, pPIZ&alpha;, pBR322 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-25">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 25 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 25 -->
−
       <h2>06-19: Meeting with Doctors Without Borders, regional manager</h2>
+
    <div class="week" id="week-25">
−
      <p>
+
       <section>
−
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a5">her testimony here.</a>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 25 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </p>
+
       </section>
−
      <h2>06-20: Cholera survey</h2>
+
      <section class="week-ihp">
−
      <p>
+
        <h2>06-19: Meeting with Doctors Without Borders, regional manager</h2>
−
        We shared our survey about cholera on the social media.
+
        <p>
−
        <!-- lINK -->
+
          You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a5">her testimony here.</a>
−
      </p>
+
        </p>
−
       <h2>06-22: Meeting with all the team members and supervisors</h2>
+
        <h2>06-20: Cholera survey</h2>
−
      <p><i>
+
        <p>
−
        Final choice of the global device purpose
+
          We shared our survey about cholera on the social media.
−
      </i></p>
+
          <!-- lINK -->
−
       <h2>06-23: First appearance of Sobki, our mascott!</h2>
+
        </p>
−
      <img src="https://static.igem.org/mediawiki/2017/2/2a/T--INSA-UPS_France--Logo.png" alt="">
+
       </section>
−
       <h2>Experiments</h2>
+
      <section class="week-igemers">
−
       <div class="wet-lab-notebook">
+
        <h2>06-22 Skype with NUS iGEM team</h2>
−
         <div>
+
      </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
      <section class="week-meeting">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        <h2>06-22: Meeting with all the team members and supervisors</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
        <p><i>
−
        </div>
+
          Final choice of the global device purpose
−
        <div>
+
        </i></p>
 +
       </section>
 +
      <section class="week-team">
 +
        <h2>06-23: First appearance of Sobki, our mascott!</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/2/2a/T--INSA-UPS_France--Logo.png" alt="">
 +
       </section>
 +
       <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
        <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Plasmids preparation</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Transformation of DH5&alpha; competent cells with pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Scale up of the preparative digestion, in order to have more DNA matrix.</li>
+
−
              <li>Reception of the PCR primer.</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Plasmids preparation</h2>
+
             <div>
−
            <ul>
+
              <h2>Plasmids preparation</h2>
−
               <li>Transformation of DH5&alpha; competent cells with pGAP, pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
+
              <ul>
−
              <li>Scale up of the preparative digestion, in order to have more DNA matrix.</li>
+
                <li>Transformation of DH5&alpha; competent cells with pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
−
              <li>Reception of the PCR primer.</li>
+
                <li>Scale up of the preparative digestion, in order to have more DNA matrix.</li>
−
            </ul>
+
                <li>Reception of the PCR primer.</li>
−
          </div>
+
               </ul>
−
          <div>
+
            </div>
−
            <!--<h2>Titre</h2>
+
            <div>
−
            <p>
+
              <h2>Plasmids preparation</h2>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
              <ul>
−
            </p>-->
+
                <li>Transformation of DH5&alpha; competent cells with pGAP, pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.</li>
 +
                <li>Scale up of the preparative digestion, in order to have more DNA matrix.</li>
 +
                <li>Reception of the PCR primer.</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-26">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 26 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 26 -->
−
       <h2>06-28: Meeting with the CEO of Sunwaterlife, building a collaboration</h2>
+
    <div class="week" id="week-26">
−
      <p>
+
       <section>
−
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 26 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </p>
+
       </section>
−
      <h2>06-30: First steps of our ethical matrix</h2>
+
      <section class="week-igemers">
−
      <!-- img éthique -->
+
        <h2>06-26: Skype with the iGEM team of Purdue</h2>
−
       <h2>Experiments</h2>
+
      </section>
−
       <div class="wet-lab-notebook">
+
      <section class="week-ihp">
−
         <div>
+
        <h2>06-28: Meeting with the CEO of Sunwaterlife, building a collaboration</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
        <p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
          You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
        </p>
−
         </div>
+
        <h2>06-30: First steps of our ethical matrix</h2>
−
         <div>
+
        <!-- img éthique -->
 +
       </section>
 +
       <section class="week-meeting">
 +
         <h2>06-29: 21th brainstorming</h2>
 +
      </section>
 +
      <section class="week-team">
 +
        <h2>06-30: Tropicool!</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/6/6b/T--INSA-UPS_France--Notebook_Week-26.jpg" alt="">
 +
      </section>
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
         <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Cloning VhCqsA</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <ul>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Digestion of the PCR amplicons and ligation with the appropriate cloning vector. </li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Assembly of <i>V. harveyi</i> gene circuit : starting the sub-cloning work</h2>
+
             <div>
−
            <ul>
+
              <h2>Cloning VhCqsA</h2>
−
              <li>Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part</li>
+
              <ul>
−
              <li>Digestion of the PCR amplicons and ligation with the appropriate cloning vector. </li>
+
                <li>Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part</li>
−
              <li>Cloning attempt #1 and #2 to sub-clone &nbsp;Vh1, Vh2 and &nbsp;Vh3 in their respective vector.  </li>
+
                <li>Digestion of the PCR amplicons and ligation with the appropriate cloning vector. </li>
−
            </ul>
+
              </ul>
−
          </div>
+
            </div>
−
          <div>
+
            <div>
−
            <!--<h2>Titre</h2>
+
              <h2>Assembly of <i>V. harveyi</i> gene circuit : starting the sub-cloning work</h2>
−
            <p>
+
              <ul>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part</li>
−
            </p>-->
+
                <li>Digestion of the PCR amplicons and ligation with the appropriate cloning vector. </li>
 +
                <li>Cloning attempt #1 and #2 to sub-clone &nbsp;Vh1, Vh2 and &nbsp;Vh3 in their respective vector.  </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-27">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 27 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 27 -->
−
       <h2>07-07: Delft European iGEM Meetup</h2>
+
    <div class="week" id="week-27">
−
       <!-- link meetups -->
+
       <section>
−
      <h2>Experiments</h2>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 27 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
       <div class="wet-lab-notebook">
+
       </section>
−
         <div>
+
       <section class="week-meeting">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
        <h2>07-06: 22th brainstorming</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
      <section class="week-igemers">
−
         </div>
+
         <h2>07-07: Delft European iGEM Meetup</h2>
−
         <div>
+
        <img src="https://static.igem.org/mediawiki/2017/e/e1/T--INSA-UPS_France--Collaborations_meetup-2.jpg" alt="">
 +
        <!-- link meetups -->
 +
      </section>
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
         <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Cloning VhCqsA</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <ul>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Optimization of PCR cycle </li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Cloning of VhCqsA into pSB1C3 : positives clones grew </li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Assembly of <i>V. harveyi</i> gene circuit : continuing the sub-cloning work</h2>
+
             <div>
−
            <ul>
+
              <h2>Cloning VhCqsA</h2>
−
              <li>Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map. </li>
+
              <ul>
−
              <li>New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  </li>
+
                <li>Optimization of PCR cycle </li>
−
            </ul>
+
                <li>Cloning of VhCqsA into pSB1C3 : positives clones grew </li>
−
          </div>
+
              </ul>
−
          <div>
+
            </div>
−
            <!--<h2>Titre</h2>
+
            <div>
−
            <p>
+
              <h2>Assembly of <i>V. harveyi</i> gene circuit : continuing the sub-cloning work</h2>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
              <ul>
−
            </p>-->
+
                <li>Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map. </li>
 +
                <li>New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
  
−
     <section class="week" id="week-28">
+
     <!-- Week 28 -->
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 28 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <div class="week" id="week-28">
−
       <h2>07-14: Meeting with Doctors Without Borders, cholera specialist</h2>
+
       <section>
−
     
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 28 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
       <!-- link meetups -->
+
       </section>
−
      <h2>Experiments</h2>
+
       <section class="week-meeting">
−
       <div class="wet-lab-notebook">
+
        <h2>07-13: 23th brainstorming</h2>
−
         <div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
      <section class="week-ihp">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
         <h2>07-14: Meeting with Doctors Without Borders, cholera specialist</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
      </section>
−
        </div>
+
      <section class="week-experiment">
−
        <div>
+
        <h2>Experiments</h2>
 +
        <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <!--<h2>Titre</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <p>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </p>-->
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Assembly of <i>V. harveyi</i> gene circuit : continuing the sub-cloning work</h2>
+
             <div>
−
            <ul>
+
              <!--<h2>Titre</h2>
−
              <li>Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map. </li>
+
              <p>
−
              <li>New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  </li>
+
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Repudiandae repellat, delectus ab voluptatem pariatur suscipit sed perspiciatis nisi officiis iusto nulla facere provident incidunt dolorum voluptate eligendi ut unde error?
−
            </ul>
+
              </p>-->
−
          </div>
+
            </div>
−
          <div>
+
            <div>
−
            <!--<h2>Titre</h2>
+
              <h2>Assembly of <i>V. harveyi</i> gene circuit : continuing the sub-cloning work</h2>
−
            <p>
+
              <ul>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map. </li>
−
            </p>-->
+
                <li>New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-29">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 29 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 29 -->
−
       <h2>07-21: Parisian meetup</h2>
+
    <div class="week" id="week-29">
−
       <h2>Experiments</h2>
+
       <section>
−
            <div class="wet-lab-notebook">
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 29 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
         <div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
      <section class="week-meeting">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        <h2>07-20: 24th brainstorming</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       </section>
−
         </div>
+
      <section class="week-igemers">
−
        <div>
+
         <h2>07-21: Parisian meetup</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/8/8b/T--INSA-UPS_France--Collaborations_meetup-1.jpg" alt="">
 +
        <h2>07-23: Skype with the iGEM team of Greece</h2>
 +
      </section>
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
              <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Expressing VhCqsA (2nd)</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>SDS page fo the protein overexpression of our clones (fail)</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Assembly of <i>V. harveyi</i> gene circuit : A new strategy</h2>
+
             <div>
−
            <ul>
+
              <h2>Expressing VhCqsA (2nd)</h2>
−
              <li>Amplification of the IDT gblocks, digestion of the PCR amplicons. </li>
+
              <ul>
−
              <li>Amplification and digestion of the sub-cloning vectors. </li>
+
                <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
−
              <li>Ligation and transformation in <i>E. coli</i> STELLAR competent cells (attempt #8). </li>
+
                <li>SDS page fo the protein overexpression of our clones (fail)</li>
−
            </ul>
+
              </ul>
 +
            </div>
 +
            <div>
 +
              <h2>Assembly of <i>V. harveyi</i> gene circuit : A new strategy</h2>
 +
              <ul>
 +
                <li>Amplification of the IDT gblocks, digestion of the PCR amplicons. </li>
 +
                <li>Amplification and digestion of the sub-cloning vectors. </li>
 +
                <li>Ligation and transformation in <i>E. coli</i> STELLAR competent cells (attempt #8). </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 +
        </div>     
 +
      </section>
 +
    </div>
 +
 +
 +
    <!-- Week 30 -->
 +
    <div class="week" id="week-30">
 +
      <section>
 +
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 30 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 +
      </section>
 +
      <section class="week-ihp">
 +
        <h2>07-27: Meeting at TWB</h2>
 +
        <p>
 +
          You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a2">their testimony here.</a>
 +
        </p>
 +
      </section>
 +
      <section class="week-team">
 +
        <h2>07-29: Team building in Pyrenees</h2>
 +
        <img src="https://static.igem.org/mediawiki/2017/e/ec/T--INSA-UPS_France--Notebook_Week-30.gif" alt="">
 +
      </section>
 +
      <section class="week-experiment">
 +
        <h2>Experiments</h2>
 +
              <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <!--<h2>Titre</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <p>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </p>-->
+
 
           </div>
 
           </div>
−
        </div>
 
−
      </div>     
 
−
    </section>
 
−
    <section class="week" id="week-30">
 
−
      <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 30 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
 
−
      <h2>07-27: Meeting at TWB</h2>
 
−
      <p>
 
−
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a2">their testimony here.</a>
 
−
      </p>
 
−
      <h2>Experiments</h2>
 
−
            <div class="wet-lab-notebook">
 
−
        <div>
 
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
 
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
 
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
 
−
        </div>
 
−
        <div>
 
 
           <div>
 
           <div>
−
             <h2>Expressing VhCqsA (3rd)</h2>
+
             <div>
−
            <ul>
+
              <h2>Expressing VhCqsA (3rd)</h2>
−
              <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
+
              <ul>
−
            </ul>
+
                <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
−
          </div>
+
              </ul>
−
          <div>
+
            </div>
−
            <h2>Assembly of <i>V. harveyi</i> gene circuit : The first transformant</h2>
+
            <div>
−
            <ul>
+
              <h2>Assembly of <i>V. harveyi</i> gene circuit : The first transformant</h2>
−
              <li>TA cloning (attempt #9): amplification of the IDT gblocks with KAPA2G polymerase , digestion of the PCR amplicons. Ligation with pGEM vector TA cloning vector. </li>
+
              <ul>
−
              <li>Analysis of the transformants (attempt #8 and #9)  by checking the restriction map. </li>
+
                <li>TA cloning (attempt #9): amplification of the IDT gblocks with KAPA2G polymerase , digestion of the PCR amplicons. Ligation with pGEM vector TA cloning vector. </li>
−
              <li>The part 1 and 2 were successfully cloned in pBR322 and pGEM.  </li>
+
                <li>Analysis of the transformants (attempt #8 and #9)  by checking the restriction map. </li>
−
            </ul>
+
                <li>The part 1 and 2 were successfully cloned in pBR322 and pGEM.  </li>
−
          </div>
+
              </ul>
−
          <div>
+
            </div>
−
            <!--<h2>Titre</h2>
+
            <div>
−
            <p>
+
              <!--<h2>Titre</h2>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
              <p>
−
            </p>-->
+
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
 
      
 
      
−
     <section class="week" id="week-31">
+
     <!-- Week 31 -->
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 31 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <div class="week" id="week-31">
−
       <h2>Experiments</h2>
+
       <section>
−
            <div class="wet-lab-notebook">
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 31 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
         <div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
      <section class="week-meeting">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
         <h2>08-01: 25th brainstorming</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
      </section>
−
         </div>
+
      <section class="week-igemers">
−
        <div>
+
        <h2>08-07: Skype with the iGEM team of Boston</h2>
 +
      </section>
 +
      <section class="week-experiment">
 +
         <h2>Experiments</h2>
 +
              <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Expressing VhCqsA (aborted) + Cloning of VcCqsA </h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR aborted : no growth of bacteria</li>
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Step 2 : reconstruction of CqsS Rc by ligating Vh2 to Vh1 in pBR322. </h2>
+
             <div>
−
            <ul>
+
              <h2>Expressing VhCqsA (aborted) + Cloning of VcCqsA </h2>
−
              <li>Storage of the transformants with Vh1 and Vh2 in glycerol at -80&deg;C.</li>
+
              <ul>
−
              <li>Amplification, digestion of pBR322-Vh1,  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt A)</li>
+
                <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR aborted : no growth of bacteria</li>
−
              <li>Transformation of Vh3 (attempt #10) in <i>E. coli</i> DH5&alpha; competent cells </li>
+
              </ul>
−
              <li>Analysis of the transformants (attempt #A and #10)  by checking the restriction map.</li>
+
            </div>
−
            </ul>
+
            <div>
−
          </div>
+
              <h2>Step 2 : reconstruction of CqsS Rc by ligating Vh2 to Vh1 in pBR322. </h2>
−
          <div>
+
              <ul>
−
            <!--<h2>Titre</h2>
+
                <li>Storage of the transformants with Vh1 and Vh2 in glycerol at -80&deg;C.</li>
−
            <p>
+
                <li>Amplification, digestion of pBR322-Vh1,  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt A)</li>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Transformation of Vh3 (attempt #10) in <i>E. coli</i> DH5&alpha; competent cells </li>
−
            </p>-->
+
                <li>Analysis of the transformants (attempt #A and #10)  by checking the restriction map.</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       
−
     
+
       </section>
−
     </section>
+
     </div>
  
−
     <section class="week" id="week-32">
+
     <!-- Week 32 -->
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 32 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <div class="week" id="week-32">
−
       <h2>Experiments</h2>
+
       <section>
−
       <div class="wet-lab-notebook">
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 32 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
         <div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
       <section class="week-experiment">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
         <h2>Experiments</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
        <div class="wet-lab-notebook">
−
        </div>
+
−
        <div>
+
−
          <div>
+
−
            <h2>Expressing VhCqsA (4th time) + VcCqsA cloning</h2>
+
−
            <ul>
+
−
              <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
+
−
              <li>PCR on VcCqsA</li>
+
−
            </ul>
+
−
          </div>
+
 
           <div>
 
           <div>
−
             <h2>Step 2 CqsS Rc assembly in progress…still no success for Vh3</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt B and C)</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Transformation of Vh3 (attempt #11 to #13) in <i>E. coli</i> TOP10 competent cells using an alternative restriction enzyme :  PstI-HF restriction enzyme is remplaced  by SpeI.</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <!--<h2>Titre</h2>
+
             <div>
−
            <p>
+
              <h2>Expressing VhCqsA (4th time) + VcCqsA cloning</h2>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
              <ul>
−
            </p>-->
+
                <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
 +
                <li>PCR on VcCqsA</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <h2>Step 2 CqsS Rc assembly in progress…still no success for Vh3</h2>
 +
              <ul>
 +
                <li>Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt B and C)</li>
 +
                <li>Transformation of Vh3 (attempt #11 to #13) in <i>E. coli</i> TOP10 competent cells using an alternative restriction enzyme :  PstI-HF restriction enzyme is remplaced  by SpeI.</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-33">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 33 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 33 -->
−
       <h2>Experiments</h2>
+
    <div class="week" id="week-33">
−
       <div class="wet-lab-notebook">
+
       <section>
−
         <div>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 33 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
       <section class="week-experiment">
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
         <h2>Experiments</h2>
−
        </div>
+
        <div class="wet-lab-notebook">
−
        <div>
+
 
           <div>
 
           <div>
−
             <h2>Cloning of VcCqsA</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>From ligation to transformation : transformants grew.</li>
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <h2>Step 2 CqsS Rc assembly in progress, preparation of the conjugation</h2>
+
             <div>
−
            <ul>
+
              <h2>Cloning of VcCqsA</h2>
−
              <li>Analysis of the transformants (attempt #13) &nbsp;by checking the restriction map : the part 3 is successfully cloned in pSB1C3. The transformant is stored &nbsp;in glycerol at -80&deg;C.</li>
+
              <ul>
−
              <li>Amplification of pBBR1MCS-4 and pBBR1MCS-5 for cloning of Vh3 and for a conjugation assay. </li>
+
                <li>From ligation to transformation : transformants grew.</li>
−
              <li>Ligation of Vh3 in pBBR1MCS-4 and transformation in <i>E. coli</i> TOP10 (attempt &alpha;). </li>
+
              </ul>
−
              <li>Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt D to F) </li>
+
            </div>
−
            </ul>
+
            <div>
−
          </div>
+
              <h2>Step 2 CqsS Rc assembly in progress, preparation of the conjugation</h2>
−
          <div>
+
              <ul>
−
            <!--<h2>Titre</h2>
+
                <li>Analysis of the transformants (attempt #13) &nbsp;by checking the restriction map : the part 3 is successfully cloned in pSB1C3. The transformant is stored &nbsp;in glycerol at -80&deg;C.</li>
−
            <p>
+
                <li>Amplification of pBBR1MCS-4 and pBBR1MCS-5 for cloning of Vh3 and for a conjugation assay. </li>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Ligation of Vh3 in pBBR1MCS-4 and transformation in <i>E. coli</i> TOP10 (attempt &alpha;). </li>
−
            </p>-->
+
                <li>Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in <i>E. coli</i> TOP10 competent cells (attempt D to F) </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-34">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 34 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
 
−
       <h2>Experiments</h2>
+
     <!-- Week 34 -->
−
       <div class="wet-lab-notebook">
+
    <div class="week" id="week-34">
−
         <div>
+
       <section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 34 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       <section class="week-experiment">
−
        </div>
+
         <h2>Experiments</h2>
−
        <div>
+
        <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Confirming VcCqsa clone + Expressing VhCqsA (5th time)</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <ul>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Transformant were good</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
              
+
             <div>
−
            <ul>
+
              <h2>Confirming VcCqsa clone + Expressing VhCqsA (5th time)</h2>
−
               <li>Analysis of the transformants (attempt #F) &nbsp;by checking the restriction map : the part Vh1+Vh2 is successfully cloned in pBR322. The transformant is stored &nbsp;in glycerol at -80&deg;C.</li>
+
              <ul>
−
              <li>Amplification, digestion of pBR322-Vh1+Vh2, pSB1C3-Vh3. Ligation and transformation (attempt #1 and #2) in <i>E. coli</i> TOP10 competent cells. </li>
+
                <li>Transformant were good</li>
−
              <li>Ligation of Vh3 in pBBR1MCS-4 and transformation in <i>E. coli</i> TOP10 (attempt &beta; and &gamma;)</li>
+
                <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
−
              <li>Vh3 part validation : diacetyl production assay by NMR detection with <i>E. coli</i> BL21. </li>
+
               </ul>
−
            </ul>
+
            </div>
−
          </div>
+
            <div>
−
          <div>
+
             
−
            <!--<h2>Titre</h2>
+
              <ul>
−
            <p>
+
                <li>Analysis of the transformants (attempt #F) &nbsp;by checking the restriction map : the part Vh1+Vh2 is successfully cloned in pBR322. The transformant is stored &nbsp;in glycerol at -80&deg;C.</li>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Amplification, digestion of pBR322-Vh1+Vh2, pSB1C3-Vh3. Ligation and transformation (attempt #1 and #2) in <i>E. coli</i> TOP10 competent cells. </li>
−
            </p>-->
+
                <li>Ligation of Vh3 in pBBR1MCS-4 and transformation in <i>E. coli</i> TOP10 (attempt &beta; and &gamma;)</li>
 +
                <li>Vh3 part validation : diacetyl production assay by NMR detection with <i>E. coli</i> BL21. </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-35">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 35 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 35 -->
−
       <h2>Experiments</h2>
+
    <div class="week" id="week-35">
−
       <div class="wet-lab-notebook">
+
       <section>
−
         <div>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 35 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
       <section class="week-experiment">
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
         <h2>Experiments</h2>
−
        </div>
+
        <div class="wet-lab-notebook">
−
        <div>
+
 
           <div>
 
           <div>
−
             <h2>Expressing VhCqsA (6th time) + Bioluminescence</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
+
            <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
              <li>Optimisation of <i>Vibrio harveyi</i> JMH626 bioluminescent assay</li>
+
−
            </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <ul>
+
             <div>
−
               <li>2nd  diacetyl production assay by NMR detection with <i>E. coli</i> BL21.</li>
+
              <h2>Expressing VhCqsA (6th time) + Bioluminescence</h2>
−
              <li>Success of the conjugation assay of pBBR1MCS-4. </li>
+
              <ul>
−
            </ul>
+
                <li>Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)</li>
−
          </div>
+
                <li>Optimisation of <i>Vibrio harveyi</i> JMH626 bioluminescent assay</li>
−
          <div>
+
               </ul>
−
            <!--<h2>Titre</h2>
+
            </div>
−
            <p>
+
            <div>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
              <ul>
−
            </p>-->
+
                <li>2nd  diacetyl production assay by NMR detection with <i>E. coli</i> BL21.</li>
 +
                <li>Success of the conjugation assay of pBBR1MCS-4. </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-36">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 36 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
    <!-- Week 36 -->
−
       <h2>09-04: Start of our Ulule campaign</h2>
+
     <div class="week" id="week-36">
−
      <p>
+
       <section>
−
        We started a crowdfunding campaign on Ulule, a french platform. <!-- link -->
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 36 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </p>
+
       </section>
−
       <h2>Experiments</h2>
+
      <section class="week-team">
−
       <div class="wet-lab-notebook">
+
        <h2>09-04: Start of our Ulule campaign</h2>
−
         <div>
+
        <p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
          We started a crowdfunding campaign on Ulule, a french platform. <!-- link -->
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        </p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       </section>
−
        </div>
+
       <section class="week-experiment">
−
        <div>
+
         <h2>Experiments</h2>
 +
        <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Expressing VhCqsA (7th time)</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Production of C8-CAI-1 in minimal media, and analysis on MS (fail) extration on LLE dichloromethane and analysis on NMR (fail)</li>
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <ul>
+
             <div>
−
               <li>3rd  Diacetyl production assay by NMR detection with <i>E. coli</i> MG1655. </li>
+
              <h2>Expressing VhCqsA (7th time)</h2>
−
              <li>Amplification of pBBR1MCS-4 and pBBR1MCS-5. </li>
+
              <ul>
−
            </ul>
+
                <li>Production of C8-CAI-1 in minimal media, and analysis on MS (fail) extration on LLE dichloromethane and analysis on NMR (fail)</li>
−
          </div>
+
               </ul>
−
          <div>
+
            </div>
−
            <!--<h2>Titre</h2>
+
            <div>
−
            <p>
+
              <ul>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>3rd  Diacetyl production assay by NMR detection with <i>E. coli</i> MG1655. </li>
−
            </p>-->
+
                <li>Amplification of pBBR1MCS-4 and pBBR1MCS-5. </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-37">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 37 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 37 -->
−
       <h2>Experiments</h2>
+
    <div class="week" id="week-37">
−
       <div class="wet-lab-notebook">
+
       <section>
−
         <div>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 37 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
       </section>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
       <section class="week-experiment">
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
         <h2>Experiments</h2>
−
        </div>
+
        <div class="wet-lab-notebook">
−
        <div>
+
 
           <div>
 
           <div>
−
             <h2>Bioluminescence assay</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Trial on the expression of C8-CAI-1 with induction on bioluminescence on liquid media : fail</li>
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <ul>
+
             <div>
−
               <li>4th diacetyl production assay by NMR detection with <i>E. coli</i> MG1655 in M9 media supplemented with xylose and pyruvate. </li>
+
              <h2>Bioluminescence assay</h2>
−
              <li>Transformation of <i>E. coli</i> TOP10 competent cells with GFP from iGEM kit</li>
+
              <ul>
−
              <li>Ligation and transformation of pBBR1MCS-4 to RFP from iGEM kit in <i>E. coli</i> TOP10 competent cells for conjugation to <i>V. harveyi</i>. </li>
+
                <li>Trial on the expression of C8-CAI-1 with induction on bioluminescence on liquid media : fail</li>
−
              <li>Preparation of the clones for sequencing. </li>
+
               </ul>
−
            </ul>
+
            </div>
−
          </div>
+
            <div>
−
          <div>
+
              <ul>
−
            <!--<h2>Titre</h2>
+
                <li>4th diacetyl production assay by NMR detection with <i>E. coli</i> MG1655 in M9 media supplemented with xylose and pyruvate. </li>
−
            <p>
+
                <li>Transformation of <i>E. coli</i> TOP10 competent cells with GFP from iGEM kit</li>
−
              Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+
                <li>Ligation and transformation of pBBR1MCS-4 to RFP from iGEM kit in <i>E. coli</i> TOP10 competent cells for conjugation to <i>V. harveyi</i>. </li>
−
            </p>-->
+
                <li>Preparation of the clones for sequencing. </li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
       </section>
−
     </section>
+
     </div>
−
     <section class="week" id="week-38">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 38 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 38 -->
−
       <h2>09-22: Meeting with UNICEF</h2>
+
    <div class="week" id="week-38">
−
      <p>
+
       <section>
−
        You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a6">Alama Keita's testimony here.</a>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 38 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
      </p>
+
       </section>
−
       <h2>Experiments</h2>
+
      <section class="week-ihp">
−
       <div class="wet-lab-notebook">
+
        <h2>09-22: Meeting with UNICEF</h2>
−
         <div>
+
        <p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
          You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a6">Alama Keita's testimony here.</a>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        </p>
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       </section>
−
        </div>
+
       <section class="week-experiment">
−
        <div>
+
         <h2>Experiments</h2>
 +
        <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <h2>Bioluminescence assay</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
            <ul>
+
            <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
              <li>Trial on the expression of C8-CAI-1 with induction on bioluminescence on plate : success</li>
+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </ul>
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <ul>
+
             <div>
−
               <li>Results of the 4th Diacetyl production assay by NMR detection: a small peak of diacetyl is visible. </li>
+
              <h2>Bioluminescence assay</h2>
−
              <li>5th diacetyl production assay by NMR detection with <i>E. coli</i> MG1655 in M9 media supplemented with xylose and pyruvate. </li>
+
              <ul>
−
              <li>Results of ligation of RFP in conjugative plasmid: all transformants are red. Two transformants are stored at -80&deg;C in order to be used for conjugation assay. </li>
+
                <li>Trial on the expression of C8-CAI-1 with induction on bioluminescence on plate : success</li>
−
              <li>Preparative work for the ligation of Vh3 in pBBR1MCS-4 with fresh material</li>
+
               </ul>
−
            </ul>
+
            </div>
−
          </div>
+
            <div>
−
          <div>
+
              <ul>
−
            <!--<h2>Titre</h2>
+
                <li>Results of the 4th Diacetyl production assay by NMR detection: a small peak of diacetyl is visible. </li>
−
            <p>
+
                <li>5th diacetyl production assay by NMR detection with <i>E. coli</i> MG1655 in M9 media supplemented with xylose and pyruvate. </li>
−
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+
                <li>Results of ligation of RFP in conjugative plasmid: all transformants are red. Two transformants are stored at -80&deg;C in order to be used for conjugation assay. </li>
−
            </p>-->
+
                <li>Preparative work for the ligation of Vh3 in pBBR1MCS-4 with fresh material</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
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 +
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 +
              </p>-->
 +
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           </div>
 
           </div>
 
         </div>
 
         </div>
−
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+
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+
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     <section class="week" id="week-39">
+
 
−
       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 39 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+
     <!-- Week 39 -->
−
       <h2>09-29: European Researcher's Night</h2>
+
    <div class="week" id="week-39">
−
      <!-- link -->
+
       <section>
−
       <h2>Experiments</h2>
+
        <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 39 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
−
       <div class="wet-lab-notebook">
+
       </section>
−
         <div>
+
      <section class="week-pe">
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
+
        <h2>09-29: European Researcher's Night</h2>
−
          <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
+
        <!-- link -->
−
          <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
+
       </section>
−
        </div>
+
       <section class="week-experiment">
−
        <div>
+
         <h2>Experiments</h2>
 +
        <div class="wet-lab-notebook">
 
           <div>
 
           <div>
−
             <!--<h2>Titre</h2>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/4e/T--INSA-UPS_France--Experiments_module-coli.png" alt=""></div>
−
             <p>
+
             <div><img src="https://static.igem.org/mediawiki/2017/4/48/T--INSA-UPS_France--Experiments_module-harveyi.png" alt=""></div>
−
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+
             <div><img src="https://static.igem.org/mediawiki/2017/9/99/T--INSA-UPS_France--Experiments_module-pichia.png" alt=""></div>
−
             </p>-->
+
 
           </div>
 
           </div>
 
           <div>
 
           <div>
−
             <ul>
+
             <div>
−
               <li>Observation by microscopy of <i>V. harveyi</i> (rfp) transformant</li>
+
               <!--<h2>Titre</h2>
−
              <li>Ligation of pBBR1MCS-4 and Vh3 at 16&deg;C overnight, and transformation in competent <i>E. coli</i> (Stellars)</li>
+
              <p>
−
            </ul>
+
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−
          </div>
+
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−
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+
            </div>
−
            <!--<h2>Titre</h2>
+
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−
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+
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−
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+
                <li>Observation by microscopy of <i>V. harveyi</i> (rfp) transformant</li>
−
            </p>-->
+
                <li>Ligation of pBBR1MCS-4 and Vh3 at 16&deg;C overnight, and transformation in competent <i>E. coli</i> (Stellars)</li>
 +
              </ul>
 +
            </div>
 +
            <div>
 +
              <!--<h2>Titre</h2>
 +
              <p>
 +
                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
 +
              </p>-->
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
−
       </div>
+
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−
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+
     </div>
−
     <section class="week" id="week-40">
+
 
−
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    <div class="week" id="week-40 ">
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       <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 41 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
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    </section>
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+
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 +
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 +
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 +
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 +
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 +
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Revision as of 09:48, 7 October 2017

Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Notebook

Here is a summary of what we did for our project, week by week. You can go on our Human Practices labbook page to know more about how each event affected us to take a decision about the future of our project.

January

1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31 1 2 3 4

February

29 30 31 1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 1 2 3 4

March

26 27 28 1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31 1

April

26 27 28 29 30 31 1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 1 2 3 4 5 6

May

30 1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31 1 2 3

June

28 29 30 31 1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 1

July

25 26 27 28 29 30 1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31 1 2 3 4 5

August

30 31 1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31 1 2

September

27 28 29 30 31 1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

October

1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31 1 2 3 4

Week 1

Our team was being recruited. Nothing happened this week!

Week 2

Our team was being recruited. Nothing happened this week!

Week 3

Our team was being recruited. Nothing happened this week!

Week 4

01-25: Kick Off Meeting

Gathering the team members and distribute the tasks... The adventure begins!

Week 5

01-30: 2th brainstorming: on the track of crocodile antimicrobial peptides...

We’ve took charge of differents accounts of the association (facebook, twitter, email, bank account, google drive…), and made a listing of 45 potential subjects for the competition.

Week 6

02-06: 3th brainstorming meeting

We’ve selected 17 subjects that we considered the most originals, the most feasible, and the most interesting. We’ve imagined to make a dressing against nosocomial infections containing bacteria that produces antimicrobial peptides from crocodile. We’ve read that crocodile peptides are not toxic for humans at microbicide quantity.

Week 7

02-16: 4th brainstorming

Only 7 subjects left! To make a safe device, we’ve thought of switch on the dressing by using spores that can be activated with the temperature.

Week 8

02-23: 5th brainstorming

We made a list of our public engagement events. For our subject, we’ve focused our research on the originality, and we’ve noted that a similar dressing has already been made by an iGEM team before. So we imagined the use of anticoagulant molecules in the dressing. We’ve thought of a bandage for pets.

Week 9

03-02: 6th brainstorming

We’ve analysed the 7 subjects, and we’ve noted some technical issues. For the “crocodile peptides project”, we’ve raised several questions : what organisme should we use to produce antimicrobial peptides? We need to find an organism which is not susceptible to their antimicrobial activity. We need to use a special membrane to contain GMOs inside the dressing and not directly in contact to the skin.

03-05: Team building at the restaurant

Our team met for the first time outside the INSA buildings and shared a delicious korean meal after brainstorming the whole afternoon!

Week 10

03-09: 7th brainstorming meeting

Dropping of first device

03-12: Brainstorming in a café

Week 11

03-14: 8th brainstorming

Week 12

03-21: 9th brainstorming

03-26: Brainstorming in a café

Week 13

03-30: 10th brainstorming

Technical solutions

Week 14

04-06: 11th brainstorming

Second device proposition

04-07: choice of the subject

Week 15

04-13: 12th brainstorming

Week 16

04-19: Meeting with Marc Lemonnier, CEO of Antabio

You can read the result of his testimony here.

04-20: 13th brainstorming

04-23: First drafts of Sobki

Week 17

04-26: 14th brainstorming

Week 18

05-02: 15th brainstorming

Week 19

05-13: Paul & Margaux officialy joined our team!

05-11: 16th brainstorming

Week 20

05-17: 17th brainstorming

05-18: iGEM distribution kit received

Week 21

05-25: Parts design day at Brice's

Week 22

05-28: Official announcement of our subject

We officialy the subject of our project on social media!

05-31: Exposciences

We took part to the Exposciences event.

06-01: Interventions in schools

Experiments

Design and orders

  • Design of the gBlocks and launching of the synthesis order for IDT
  • Design of the PCR oligos

Design and orders

  • Design of the gBlocks and launching of the synthesis order for IDT
  • Design of the PCR oligos

Week 23

06-06: Meeting with the CEO of Sunwaterlife

You can read the result of his testimony here.

06-08: 18th brainstorming

06-09: Intervention in school

Experiments

Taking care of V. harveyi

  • Reception and storage of V. harveyi BB120 (WT) and V. harveyi JMH626 (ΔcqsA ΔluxQ ΔluxN) CmR at -80 °C.
  • Investigation of V. harveyi antibiotics resistances.

Week 24

06-14: Scope statement start

Third device proposition

06-15: 19th brainstorming

06-16: Skype with Greece iGEM team

Experiments

Amplifying pSB1C3

  • Reception of IDT gBlocks and storage
  • Preparative work with the cloning vectors : Amplification and stock of pSB1C3

Taking charge of the lab and of V. harveyi

  • Growth kinetic assay in order to determine the adequate NaCl concentration for V. harveyi growth.
  • Reception of IDT gBlocks and storage
  • Transformation of DH5α competent cells with pYFP, pDsRed, pPIZα, pBR322 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.

Week 25

06-19: Meeting with Doctors Without Borders, regional manager

You can read the result of her testimony here.

06-20: Cholera survey

We shared our survey about cholera on the social media.

06-22 Skype with NUS iGEM team

06-22: Meeting with all the team members and supervisors

Final choice of the global device purpose

06-23: First appearance of Sobki, our mascott!

Experiments

Plasmids preparation

  • Transformation of DH5α competent cells with pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.
  • Scale up of the preparative digestion, in order to have more DNA matrix.
  • Reception of the PCR primer.

Plasmids preparation

  • Transformation of DH5α competent cells with pGAP, pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.
  • Scale up of the preparative digestion, in order to have more DNA matrix.
  • Reception of the PCR primer.

Week 26

06-26: Skype with the iGEM team of Purdue

06-28: Meeting with the CEO of Sunwaterlife, building a collaboration

You can read the result of his testimony here.

06-30: First steps of our ethical matrix

06-29: 21th brainstorming

06-30: Tropicool!

Experiments

Cloning VhCqsA

  • Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part
  • Digestion of the PCR amplicons and ligation with the appropriate cloning vector.

Assembly of V. harveyi gene circuit : starting the sub-cloning work

  • Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part
  • Digestion of the PCR amplicons and ligation with the appropriate cloning vector.
  • Cloning attempt #1 and #2 to sub-clone  Vh1, Vh2 and  Vh3 in their respective vector.  

Week 27

07-06: 22th brainstorming

07-07: Delft European iGEM Meetup

Experiments

Cloning VhCqsA

  • Optimization of PCR cycle
  • Cloning of VhCqsA into pSB1C3 : positives clones grew 

Assembly of V. harveyi gene circuit : continuing the sub-cloning work

  • Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map.
  • New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  

Week 28

07-13: 23th brainstorming

07-14: Meeting with Doctors Without Borders, cholera specialist

Experiments

Assembly of V. harveyi gene circuit : continuing the sub-cloning work

  • Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map.
  • New attempt (attempt #3) to sub-clone Vh1, Vh2 and  Vh3 in their respective vector.  

Week 29

07-20: 24th brainstorming

07-21: Parisian meetup

07-23: Skype with the iGEM team of Greece

Experiments

Expressing VhCqsA (2nd)

  • Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
  • SDS page fo the protein overexpression of our clones (fail)

Assembly of V. harveyi gene circuit : A new strategy

  • Amplification of the IDT gblocks, digestion of the PCR amplicons.
  • Amplification and digestion of the sub-cloning vectors.
  • Ligation and transformation in E. coli STELLAR competent cells (attempt #8).

Week 30

07-27: Meeting at TWB

You can read the result of their testimony here.

07-29: Team building in Pyrenees

Experiments

Expressing VhCqsA (3rd)

  • Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)

Assembly of V. harveyi gene circuit : The first transformant

  • TA cloning (attempt #9): amplification of the IDT gblocks with KAPA2G polymerase , digestion of the PCR amplicons. Ligation with pGEM vector TA cloning vector.
  • Analysis of the transformants (attempt #8 and #9)  by checking the restriction map.
  • The part 1 and 2 were successfully cloned in pBR322 and pGEM.  

Week 31

08-01: 25th brainstorming

08-07: Skype with the iGEM team of Boston

Experiments

Expressing VhCqsA (aborted) + Cloning of VcCqsA

  • Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR aborted : no growth of bacteria

Step 2 : reconstruction of CqsS Rc by ligating Vh2 to Vh1 in pBR322.

  • Storage of the transformants with Vh1 and Vh2 in glycerol at -80°C.
  • Amplification, digestion of pBR322-Vh1,  pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt A)
  • Transformation of Vh3 (attempt #10) in E. coli DH5α competent cells
  • Analysis of the transformants (attempt #A and #10)  by checking the restriction map.

Week 32

Experiments

Expressing VhCqsA (4th time) + VcCqsA cloning

  • Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
  • PCR on VcCqsA

Step 2 CqsS Rc assembly in progress…still no success for Vh3

  • Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt B and C)
  • Transformation of Vh3 (attempt #11 to #13) in E. coli TOP10 competent cells using an alternative restriction enzyme :  PstI-HF restriction enzyme is remplaced  by SpeI.

Week 33

Experiments

Cloning of VcCqsA

  • From ligation to transformation : transformants grew.

Step 2 CqsS Rc assembly in progress, preparation of the conjugation

  • Analysis of the transformants (attempt #13)  by checking the restriction map : the part 3 is successfully cloned in pSB1C3. The transformant is stored  in glycerol at -80°C.
  • Amplification of pBBR1MCS-4 and pBBR1MCS-5 for cloning of Vh3 and for a conjugation assay.
  • Ligation of Vh3 in pBBR1MCS-4 and transformation in E. coli TOP10 (attempt α).
  • Amplification, digestion of pBR322-Vh1  pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt D to F)

Week 34

Experiments

Confirming VcCqsa clone + Expressing VhCqsA (5th time)

  • Transformant were good
  • Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
  • Analysis of the transformants (attempt #F)  by checking the restriction map : the part Vh1+Vh2 is successfully cloned in pBR322. The transformant is stored  in glycerol at -80°C.
  • Amplification, digestion of pBR322-Vh1+Vh2, pSB1C3-Vh3. Ligation and transformation (attempt #1 and #2) in E. coli TOP10 competent cells.
  • Ligation of Vh3 in pBBR1MCS-4 and transformation in E. coli TOP10 (attempt β and γ)
  • Vh3 part validation : diacetyl production assay by NMR detection with E. coli BL21.

Week 35

Experiments

Expressing VhCqsA (6th time) + Bioluminescence

  • Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
  • Optimisation of Vibrio harveyi JMH626 bioluminescent assay
  • 2nd  diacetyl production assay by NMR detection with E. coli BL21.
  • Success of the conjugation assay of pBBR1MCS-4.

Week 36

09-04: Start of our Ulule campaign

We started a crowdfunding campaign on Ulule, a french platform.

Experiments

Expressing VhCqsA (7th time)

  • Production of C8-CAI-1 in minimal media, and analysis on MS (fail) extration on LLE dichloromethane and analysis on NMR (fail)
  • 3rd  Diacetyl production assay by NMR detection with E. coli MG1655.
  • Amplification of pBBR1MCS-4 and pBBR1MCS-5.

Week 37

Experiments

Bioluminescence assay

  • Trial on the expression of C8-CAI-1 with induction on bioluminescence on liquid media : fail
  • 4th diacetyl production assay by NMR detection with E. coli MG1655 in M9 media supplemented with xylose and pyruvate.
  • Transformation of E. coli TOP10 competent cells with GFP from iGEM kit
  • Ligation and transformation of pBBR1MCS-4 to RFP from iGEM kit in E. coli TOP10 competent cells for conjugation to V. harveyi.
  • Preparation of the clones for sequencing.

Week 38

09-22: Meeting with UNICEF

You can read the result of Alama Keita's testimony here.

Experiments

Bioluminescence assay

  • Trial on the expression of C8-CAI-1 with induction on bioluminescence on plate : success
  • Results of the 4th Diacetyl production assay by NMR detection: a small peak of diacetyl is visible.
  • 5th diacetyl production assay by NMR detection with E. coli MG1655 in M9 media supplemented with xylose and pyruvate.
  • Results of ligation of RFP in conjugative plasmid: all transformants are red. Two transformants are stored at -80°C in order to be used for conjugation assay.
  • Preparative work for the ligation of Vh3 in pBBR1MCS-4 with fresh material

Week 39

09-29: European Researcher's Night

Experiments

  • Observation by microscopy of V. harveyi (rfp) transformant
  • Ligation of pBBR1MCS-4 and Vh3 at 16°C overnight, and transformation in competent E. coli (Stellars)

Week 40

Week 41

Week 42

Week 43

Week 44