Difference between revisions of "Team:Aix-Marseille/Parts"
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During the summer, several basic parts were constructed. Some of them are not built using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard, but rather with the [http://parts.igem.org/Assembly_standard_25 Rfc25], in order to construct fusion proteins. | During the summer, several basic parts were constructed. Some of them are not built using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard, but rather with the [http://parts.igem.org/Assembly_standard_25 Rfc25], in order to construct fusion proteins. | ||
| − | '''We created | + | '''We created a generally usable basic part: ''' |
*''Xylella fastidiosa'' constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004] | *''Xylella fastidiosa'' constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004] | ||
| − | |||
'''We designed 3 parts for the quorum sensing approach:''' | '''We designed 3 parts for the quorum sensing approach:''' | ||
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*Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002] | *Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002] | ||
| − | '''We designed | + | '''We designed 2 part to disrupt biofilms:''' |
| + | *Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003] | ||
*EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006] | *EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006] | ||
Revision as of 22:28, 13 October 2017
Parts
During the summer, several basic parts were constructed. Some of them are not built using the [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard, but rather with the [http://parts.igem.org/Assembly_standard_25 Rfc25], in order to construct fusion proteins.
We created a generally usable basic part:
- Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
We designed 3 parts for the quorum sensing approach:
- Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
- Acyl-CoA isomerase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
- Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]
We designed 2 part to disrupt biofilms:
- Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]
- EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]
Finally, we designed some basic parts for the phage-like particle approach:
- Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
- Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]
Then a collection of parts to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a collection of targeting parts:
- p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
- p3_ N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
- p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
- p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
- p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
- p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
- p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
- p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
- p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
- p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
- p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]
