Difference between revisions of "Team:MSU-Michigan/Current"

(Created page with "{{MSU-Michigan}} <h1>Current from Induction</h1>")
 
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<h1>Current from Induction</h1>
 
<h1>Current from Induction</h1>
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3>
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<img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png" width="366.5" height="300">
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<p>Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.</p>
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<h3>deltamtrB_GFPmtrB Induced V Uninduced</h3>
 +
<img src="https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png" width="366.5" height="300">
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<p>The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.</p>
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<h3>Multiple Strains</h3>
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<img src="https://static.igem.org/mediawiki/2017/d/d7/T--MSU-Michigan--current170714.png" width="366.5" height="300">
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<p>Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.</p>
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<h3>Anaerobic Current</h3>
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<img src="https://static.igem.org/mediawiki/2017/2/22/T--MSU-Michigan--currentanaerobic20170911.png" width="366.5" height="300">
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<p>Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.</p>

Revision as of 21:41, 14 October 2017


Current from Induction

deltamtrB_GFPmtrB Induced V Uninduced

<img src="T--MSU-Michigan--currentmtrB170725.png" width="366.5" height="300">

Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.

deltamtrB_GFPmtrB Induced V Uninduced

<img src="T--MSU-Michigan--currentdeltamrtBGFPinduced.png" width="366.5" height="300">

The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.

Multiple Strains

<img src="T--MSU-Michigan--current170714.png" width="366.5" height="300">

Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.

Anaerobic Current

<img src="T--MSU-Michigan--currentanaerobic20170911.png" width="366.5" height="300">

Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.