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Revision as of 06:02, 15 October 2017
Material&Methods
- Parts
- Primer list
- Materials
- 3-1 Kit
- 3-2 Restricton Enzyme
- 3-3 Polymerase
- 3-4 DNA ligase
- 3-5 Marker
- 3-6 Organism
- 3-7 Antibiotics
- 3-8 Equipment
- 3-9 Backborns
- 3-10 Buffer
- 3-11 SDSPAGE/Western blotting
- Methods
- 4-1 Miniprep
- 4-2 Gel Extraction
- 4-3 Restrict Enzyme Digestion
- 4-4 Ligation
- 4-5 Transformation
- 4-6 PCR
- 4-7 Sequencing
- 4-8 RT-PCR
- 4-10 Western blotting(Membrane fraction)
Parts
Primer list
| Primer name | Sequence | Length | Tm | GC% | Designer | Manufacturer |
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Materials
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