Difference between revisions of "Wiki/Testing/protocolsmethods"

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<div data-scroll-id="note-part2220" class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width: 0px;"></div><div contenteditable="false" class="editable selectable is-locked" data-cid="note-part2220" data-index="23" data-allow-multiline="false" data-allow-at-search="true" style="margin-left: 0px;"><span style="color:#5548CE">Participants: Julian, Jorge, Milica, Dawa, Erika (I hope I didnt forget anyone)</span></div></div></div>
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<ul class="myEntry other">
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    <li class="myTitel">
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    <b>RNA Adsorption Test on first 3D print</b>
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    </li>
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    <li class="myProtocol">
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    Protocol:  <a href="#">Total RNA Purification with Norgen Kit</a>
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    </li>
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    <li class="myParticipants">
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    Participants: Jorge
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    </li>
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    <li class="myObservations">
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    Notes:
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    <ul>
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                        <li> Total RNA sample 5 used, prepared on Friday 02/06 by Jorge (Concentration: 126 ng/µl)</li>
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                        <li> Procedure:
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                            <ul>
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<li>Take 1 µl presample from stock ; add with 2 µl nf H2O and 3 µl 2x RNA LD</li>
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<li>Put 5 µl of sample on device</li>
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<li>Take 1 µl of sample immediately, add with 2 µl nf H2O and 3 µl 2x RNA LD</li>
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<li>Wait 15 minutes</li>
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<li>Take 1 µl of sample immediately, add with 2 µl nf H2O and 3 µl 2x RNA LD</li>
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<li>Store at -80 °C</li>
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<li> What still needs to be done:
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<ul>
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<li>
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Cook samples at 95 °C for 10 minutes and put directly on ice afterwards (to prevent refolding)
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</li>
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<li>
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Load on Gel and quantify</li>
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</ul>
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</ul>
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    </ul>
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    </li>
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    </ul>
 
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Latest revision as of 14:37, 15 October 2017

  • RNA Adsorption Test on first 3D print
  • Protocol: Total RNA Purification with Norgen Kit
  • Participants: Jorge
  • Notes:
    • Total RNA sample 5 used, prepared on Friday 02/06 by Jorge (Concentration: 126 ng/µl)
    • Procedure:
      • Take 1 µl presample from stock ; add with 2 µl nf H2O and 3 µl 2x RNA LD
      • Put 5 µl of sample on device
      • Take 1 µl of sample immediately, add with 2 µl nf H2O and 3 µl 2x RNA LD
      • Wait 15 minutes
      • Take 1 µl of sample immediately, add with 2 µl nf H2O and 3 µl 2x RNA LD
      • Store at -80 °C
      • What still needs to be done:
        • Cook samples at 95 °C for 10 minutes and put directly on ice afterwards (to prevent refolding)
        • Load on Gel and quantify