Difference between revisions of "Team:UNC-Asheville/Attributions"

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     <h2>Agar Plate Pouring</h2>
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Autoclave<br>
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Water bath<br>
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Pipetman<br>
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- 37 g pre-mixed powder consisting of:<br>
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5 g peptone<br>
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10.0 g peptone from casein<br>
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10.0 g sodium chloride<br>
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12.0 g agar-agar<br>
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1 L Sterile H2O<br>
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Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies
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*Pro-Tip* If you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of agar.
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Autoclavable flasks
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Sterile pipettes
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Ice bucket to hold antibiotic
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Antibiotic at 1000 x concentration disolved in the appropriate liquid solvent. See table to the right for appropriate antibiotic concentrations.
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Revision as of 01:31, 17 October 2017

About Us

iGEM UNC-Asheville Attributions

Attributions

Let's find out who we need to yell at.



A Disclaimer
In comparison to most schools, this list will look sparse. Our team was composed of four motivated people, and was driven by and for students. While we received advice from many, our triumphs and failures lie squarely on our shoulders.



Autoclave
Water bath
Pipetman
- 37 g pre-mixed powder consisting of:
5 g peptone
10.0 g peptone from casein
10.0 g sodium chloride
12.0 g agar-agar
1 L Sterile H2O
Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies *Pro-Tip* If you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000 x concentration disolved in the appropriate liquid solvent. See table to the right for appropriate antibiotic concentrations.