Difference between revisions of "Team:Chalmers-Gothenburg/Notebook/protocols"

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           <h4 class="subtitle">Introduction</h4>
 
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Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with Saccharomyces cerevisiae and Escherichia coli. See the exact procedures in the <a href="https://2017.igem.org/Team:Chalmers-Gothenburg/Notebook">lab journal</a>.  
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         <h4 class="sidetitle">Protocols</h4>
 
         <h4 class="sidetitle">Protocols</h4>
 
          
 
          
 
         <h2>
 
         <h2>
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           <i>E.coli</i> protocols
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           Protocols for PCR
 
         </h2>
 
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
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           <a href="">Protocol 1</a><br>
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           <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf">Thermo Scientific DreamTaq DNA Polymerase</a><br>
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           <a href="">Protocol 2</a>
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          <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/a/a3/T--Chalmers_Gothenburg--Protocols_PrimeStar_PCR.pdf">PrimeSTAR HS DNA Polymerase</a><br>
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           <a href="">Fusion PCR</a>
 
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</div>
  
 
         <h2>
 
         <h2>
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           <i>S.cerevisiae</i> protocols
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           Protocols for purification
 
         </h2>
 
         </h2>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
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           <a href="">Protocol 1</a><br>
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           <a href="">Thermo Scientific GeneJET PCR Purification Kit</a><br>
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           <a href="">Protocol 2</a>
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           <a href="">Thermo Scientific GeneJET Gel Extraction Kit</a>
 
         </p>
 
         </p>
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</div>
 
 
 
 
  
 
         <h2>
 
         <h2>
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           DNA protocols
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           Protocols for digestion and assembly
 
         </h2>
 
         </h2>
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<div style="padding-left: 25px">
 
         <p class="text">
 
         <p class="text">
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           <a href="">Protocol 1</a><br>
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           <a href="">FastDigest Restriction Enzymes</a><br>
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           <a href="">Protocol 2</a>
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           <a href="https://static.igem.org/mediawiki/2016/9/9d/T--Chalmers_Gothenburg--Protocols_gibson_assembly.pdf">Gibson Assembly Master Mix</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/1/1d/T--Chalmers_Gothenburg--Protocols_general_DNA_insert_ligation.pdf">DNA Insert Ligation into Vector DNA</a>
 
         </p>
 
         </p>
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</div>
 
 
 
 
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        <h2>
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      </div>
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          Protocols for <i>Saccharomyces cerevisiae</i>
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        </h2>
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<div style="padding-left: 25px">
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         <p class="text">
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          <a href="">Transformation (Chemical)</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/d/de/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_yeast_electroporation.pdf">Transformation (Electroporation)</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf">Transformation (Quick version)</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/9/98/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_DNA_isolation_yeast.pdf">Isolation of genomic DNA</a>
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        </p>
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</div>
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        <h2>
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          Protocols for <i>Escherichia coli</i>
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        </h2>
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<div style="padding-left: 25px">
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        <p class="text">
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          <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf">Transformation (Heat shock)</a><br>
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          <a href="">Thermo Scientific GeneJET Plasmid Miniprep Kit</a>
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        </p>
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</div>
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        <h2>
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          Protocols for Biobricks
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        </h2>
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<div style="padding-left: 25px">
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        <p class="text">
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          <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf">BioBricks Assembly Protocol</a>
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        </p>
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</div>
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        <h2>
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          Protocols for Interlab study
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        </h2>
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<div style="padding-left: 25px">
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        <p class="text">
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          <a href="">Interlab study</a>
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        </p>
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Revision as of 11:11, 17 October 2017

Chalmers Gothenburg iGEM 2017

Notebook
Protocols

Introduction

Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with Saccharomyces cerevisiae and Escherichia coli. See the exact procedures in the lab journal.

Protocols

Protocols for PCR

Protocols for purification

Protocols for digestion and assembly

Protocols for Saccharomyces cerevisiae

Protocols for Escherichia coli

Protocols for Biobricks

Protocols for Interlab study